Recombinant Sulfolobus solfataricus DNA-binding protein 7d (sso7d)

The region for expressing recombinant Sulfolobus solfataricus sso7d contains amino acids 2-64. The theoretical molecular weight of the sso7d protein is 23.1 kDa. This sso7d protein is produced using e.coli expression system. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of sso7d, which enables a simple process of detecting and purifying the sso7d recombinant protein in the following steps.

Sulfolobus solfataricus DNA-binding protein 7d (Sso7d) is a small, highly basic protein that plays a role in DNA compaction and organization within the archaeal cell. Sso7d has a unique structural feature known as an oligo-arginine "arm" that extends from its core structure. This arm is responsible for the strong electrostatic interactions with the negatively charged phosphate backbone of DNA, facilitating the binding of Sso7d to DNA. Research on Sso7d has contributed to the understanding of archaeal chromatin structure and the mechanisms by which Sulfolobus solfataricus adapts to its harsh environments. Additionally, the DNA-binding properties of Sso7d have made it a valuable tool in biotechnological applications, such as a DNA-stabilizing agent in polymerase chain reaction (PCR) and DNA storage.