Recombinant Target of rapamycin homolog (let-363), partial

Code CSB-YP836117CXY
MSDS
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Source Yeast
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Code CSB-EP836117CXY
MSDS
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Source E.coli
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Code CSB-EP836117CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP836117CXY
MSDS
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Source Baculovirus
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Code CSB-MP836117CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
let-363
Uniprot No.
Alternative Names
let-363; CeTor; B0261.2; Target of rapamycin homolog; EC 2.7.11.1; CeTOR; Lethal protein 363
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Serine/threonine-protein kinase that regulates the mRNA translation machinery, probably by modulating the activity of translation factors such as eIF-4G and eIF-2. It may have some protein kinase activity instead of lipid kinase activity. May play a role in P-granule degradation by autophagy in somatic cells during embryogenesis. Required, during larval development, for the establishment of the proper number of germline progenitors, probably upstream of rsks-1 and ife-1. Required for larval development. May act as a mediator of lifespan regulation by insulin signaling and nutrient sensing.
Gene References into Functions
  1. Dafachronic acid signaling through let-363 is essential for diet restriction-mediated longevity. PMID: 25209682
  2. influence on lifespan PMID: 14668850
  3. C. elegans target of rapamycin (TOR) and the DAF-2/insulin-signaling pathways cross-talk with pep-2 PMID: 15155758
  4. Mutations result in dauer-like larval arrest, implying that CeTOR (let-363)regulates dauer diapause. PMID: 15253933
Subcellular Location
Nucleus.
Protein Families
PI3/PI4-kinase family
Tissue Specificity
Ubiquitous. Expressed in all major tissues and organs, including the intestine, gonads and hypodermal cells. Expressed in neurons.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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