Recombinant Tubulin gamma chain (tbg-1)

Code CSB-YP333900CXY
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP333900CXY
MSDS
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Source E.coli
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Code CSB-EP333900CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333900CXY
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP333900CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tbg-1
Uniprot No.
Alternative Names
tbg-1; F58A4.8; Tubulin gamma chain; Gamma-tubulin
Species
Caenorhabditis elegans
Expression Region
1-444
Target Protein Sequence
MSGTGALMTV HVGQCGNQLA QAFWKSMVDE HGINERGQTT HEDDMNDKKD LLFYQADDDH YVPRAVLVDL EPRVINGMMQ SPNFSNLFNT DNIFMSDHGG GAGNNWASGY CQGQEVQEKI MDIIIREAEN TNNLDGILFT HSVSGGTGSG TGSLLLERLR EAFPKKVIQT YSVFANSDTS TDVVVHPYNW VLSMQRLIEN PDHVVVLDNA ALHRLAAGKF KTDTPTFDHI NSLVARIMST STAPYRFNSA MCPSIRYLDL APFPPMHFIQ SAISPVVDPN ENFTRKTSVA DVTRFLLKPT SMMVSTASRV RPNDCMLSAY MFLQGQIEAH TIMTAEQNVD FAIRRPPFYM LKPLRMMHAP LSPYVRPQYK VSGLLLNNST SVAPLFESLL SKYDKLRSKR AFIDKFEKID NFSLDMMDDA MHIVQDLLDE YKAVVQKDYL TRGL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly.
Gene References into Functions
  1. These results show that NOCA-1 acts with gamma-tubulin to assemble non-centrosomal arrays in multiple tissues and highlight functional overlap between the ninein and Patronin protein families. PMID: 26371552
  2. Data show expression of tbg-1 during the development revealed differential expression in different developmental stages and that gamma-tubulin expression was observed through out the development from embryonic and post-embryonic stages. PMID: 17160625
  3. Gamma-tubulin in the PCM stabilizes the nascent daughter centriole by promoting microtubule addition to its outer wall. Such a mechanism may help restrict new centriole assembly to the vicinity of preexisting parent centrioles that recruit PCM. PMID: 18299348
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell junction, hemidesmosome. Cell junction, adherens junction.
Protein Families
Tubulin family
Database Links

KEGG: cel:CELE_F58A4.8

STRING: 6239.F58A4.8.2

UniGene: Cel.19457

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