Recombinant Vaccinia virus Complement control protein C3 (VACWR025)

Code CSB-EP302389VAI
MSDS
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Source E.coli
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Code CSB-EP302389VAI-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP302389VAI
MSDS
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Source Baculovirus
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Code CSB-MP302389VAI
MSDS
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Source Mammalian cell
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
VACWR025
Uniprot No.
Alternative Names
VACWR025; C3L; Complement control protein C3; 28 kDa protein; Secretory protein 35; Protein C3; VCP
Species
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Expression Region
20-263
Target Protein Sequence
C CTIPSRPINM KFKNSVETDA NANYNIGDTI EYLCLPGYRK QKMGPIYAKC TGTGWTLFNQ CIKRRCPSPR DIDNGQLDIG GVDFGSSITY SCNSGYHLIG ESKSYCELGS TGSMVWNPEA PICESVKCQS PPSISNGRHN GYEDFYTDGS VVTYSCNSGY SLIGNSGVLC SGGEWSDPPT CQIVKCPHPT ISNGYLSSGF KRSYSYNDNV DFKCKYGYKL SGSSSSTCSP GNTWKPELPK CVR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Serves to protect the virus against complement attack by inhibiting both classical and alternative pathways of complement activation. Binds C3b and C4b.
Gene References into Functions
  1. These data suggest that glycosylation did not affect the human C3 binding activity of vaccinia virus complement control protein and thus may not have contributed to the attenuation of the mutant LC16m8 virus. PMID: 25030055
  2. These results suggest that complement control protein contributes to virulence by dampening both antibody and T cell responses. PMID: 21191012
Subcellular Location
Virion membrane; Peripheral membrane protein. Host cell membrane; Peripheral membrane protein; Extracellular side. Secreted. Note=Component of extracellular enveloped virus (EEV) but not intracellular mature virus (IMV). Anchored to the surface of the outermost membrane of EEV via its interaction with A56 protein.
Protein Families
Receptors of complement activation (RCA) family
Database Links

KEGG: vg:3707640

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