Recombinant Xenopus laevis Forkhead box protein D3-A (foxd3-a)

Code CSB-YP866767XBE
MSDS
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Source Yeast
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Code CSB-EP866767XBE
MSDS
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Source E.coli
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Code CSB-EP866767XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866767XBE
MSDS
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Source Baculovirus
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Code CSB-MP866767XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
foxd3-a
Uniprot No.
Alternative Names
foxd3-a; fkh6; foxd3aForkhead box protein D3-A; FoxD3-A; FoxD3a; Fork head domain-related protein 6; FKH-6; Forkhead protein 6; xFD-6; xFKH6
Species
Xenopus laevis (African clawed frog)
Expression Region
1-371
Target Protein Sequence
MTLSGSGSAS DMSGQTVLSA DDADIDVVGE GDEALDKDSE CESTAGHTDE VGELGGKEIP RSPSGSGTEA EGKGESQQQQ QEGIQNKPKN SLVKPPYSYI ALITMSILQS PQKKLTLSGI CEFISNRFPY YREKFPAWQN SIRHNLSLND CFVKIPREPG NPGKGNYWTL DPQSEDMFDN GSFLRRRKRF KRQQQDSLRE QTALMMQSFG AYSLASPYGR HYGLHPAAYT HPAALQYPYI PPVGHMLPPA VPLLPSSELT RKAFSSQLSP SLQLQLSSLS STAASIIKSE PSSRPSFSIE NIIGVSAASS AAPHTFLRPP VTVQSALMSH QPLALSRSTA AIGPILSVPT NLISGQFLPT AAAAVAKWPA Q
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional repressor that is an essential upstream regulator of neural crest determination and mesoderm induction. Functions by recruiting the transcriptional corepressor tle4. Also acts in a negative auto-regulator loop by inhibiting the transcription of its own gene.
Gene References into Functions
  1. results indicate that tcf1 acts as an essential activator of foxd3, which is critical for dorsal mesoderm formation in early embryos PMID: 23585352
  2. findings show that FoxD3 is required for Nodal expression in the Spemann organizer and this function is essential for dorsal mesoderm formation PMID: 17092955
  3. FoxD3 recruitment of Groucho corepressors is essential for the transcriptional repression of target genes and induction of mesoderm in Xenopus PMID: 17138566
Subcellular Location
Nucleus.
Tissue Specificity
Initially located within the dorsal blastopore lip (Spemann organizer) at the mid-gastrula stage. As expression fades in the neuroectoderm, expression begins in the head mesoderm in two regions at the lateral borders of the presumptive neural plate. These
Database Links

KEGG: xla:373548

UniGene: Xl.525

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