Recombinant Xenopus tropicalis Protein Wnt-11(wnt11)

Code CSB-YP026131XBF
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Source Yeast
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Code CSB-EP026131XBF
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Source E.coli
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Code CSB-EP026131XBF-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026131XBF
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Source Baculovirus
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Code CSB-MP026131XBF
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names wnt11
Uniprot No. B2GUT4
Alternative Names wnt11; wnt11r; Protein Wnt-11; Protein Wnt-11-related
Species Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Expression Region 23-352
Target Protein Sequence IKWLALAK TPLSLALNQS QHCKQLEGLV SSQMQLCRSN LELMQTIIHA AKEVKKTCIK AFTDMRWNCS SIELAPTFHQ DLERGTRESA FVYALSAAAI SHTIARACTT GDIPGCSCAP IPGESPGPGY RWGGCADNLN YGILMGSKFS DAPMKMKKSG SQANKLMHLH NSEVGRQVLK ASLEMKCKCH GVSGSCSIKT CWRGLQELRE IALDLKTKYL SATKVVHRPM GTRKQLVPKD IDIRPVQETE MIYLQSSPDY CLKNEKMGSH GTHERQCNKT SNGSDSCDLM CCGRGYNPYM DKVVERCHCK YHWCCYVTCK KCERTVERYV CK
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Shares much functionality with wnt11b. Signals through a non-canonical Wnt pathway to activate Jun-N-terminal kinase (JNK) to regulate gastrulation movements. Acts in a non-cell-autonomous manner to control neural crest migration, probably acting as an extracellular signal from surrounding tissue, but is not required for neural crest induction. Acts redundantly with wnt11b during pronephros induction. Regulates cardiac morphogenesis through the activation of JNK, but is not required for cardiac differentiation. Essential for dorsal fin development; required for an epithelial to mesenchymal transformation event prior to migration of cells into the fin, and ultimately for maintenance of fin structure. Mediates dorsal fin development through a non-canonical pathway mediated by Ca(2+).
Subcellular Location Secreted, extracellular space, extracellular matrix.
Protein Families Wnt family
Database Links

KEGG: xtr:100158655

STRING: 8364.ENSXETP00000042046

UniGene: Str.57693

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