Recombinant Zinc finger protein mex-5 (mex-5)

Code CSB-YP895023CXY
MSDS
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Source Yeast
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Code CSB-EP895023CXY
MSDS
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Source E.coli
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Code CSB-EP895023CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895023CXY
MSDS
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Source Baculovirus
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Code CSB-MP895023CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mex-5
Uniprot No.
Alternative Names
mex-5; W02A2.7Zinc finger protein mex-5
Species
Caenorhabditis elegans
Expression Region
1-468
Target Protein Sequence
MKAASNSVSS AGGSVSPTTT QPPLPPGQSS HPQIYDQQMQ YYFAAAMPNQ PMATYAAQNG SSQQYAPAAP YYQDANGQYV QVPANGSMAP QQHMMVSGQP YLYMAQPQQG AQQVMQSGQP QLIYYQQSMA PQAAPMYFHP MQAAPMLPEQ MGVMPHTQPA IPPQQQPRQV GVEISSTRTA PLTSSTPLPT SLEYETVQRD NRNRNIQFRY HRVMEHDELP IDEISKITLD NHNDDTMSAE KENHFHEHRG EKFGRRGFPI PETDSQQPPN YKTRLCMMHA SGIKPCDMGA RCKFAHGLKE LRATDAPARY PNNKYKTKLC KNFARGGTGF CPYGLRCEFV HPTDKEFQNI PPYQRMSHDD QDYDQDVIPE DYVVARHQPR FMRTGGRATT PTKVMLKHRN VAGSMMCLSN AGRDLQAGGD YNQPESNEDD LPPHLRRNRR ENPPMNKRRT SLSTKWTSEE NLGLRGHY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Functions with mex-6 to affect embryonic viability, establish soma germline asymmetry in embryos and establish plk-1, pie-1, mex-1, and pos-1 asymmetry in embryos. Also affects formation of intestinal cells. Binds to mRNA in vitro, and inhibits pgl-3-mediated P-granule formation, probably by competing with pgl-3 for binding to mRNA.
Gene References into Functions
  1. Antagonistic activities of PAR-1 and PP2A regulate MEX-5 diffusion to establish the MEX-5 protein gradient along the anterior-to-posterior axis of the one-cell C. elegans embryo. PMID: 21925318
  2. asymmetric cortically localized PAR proteins mediate the anterior enrichment of MEX-5 by reversibly changing its diffusion rate at spatially distinct points in the embryo PMID: 20627961
  3. analysis of RNA recognition by the embryonic polarity determinant MEX-5 PMID: 17264081
  4. vivo. PAR-1 and PAR-4 kinase activities are required for the phosphorylation of S458, providing a link between PAR polarity proteins and the cytoplasmic asymmetry of MEX-5 PMID: 18842813
Subcellular Location
Cytoplasm.
Tissue Specificity
Asymmetrically localized to the anterior of the zygote before mitotic division, then differentially distributed to the somatic blastomere precursor cells.
Database Links

KEGG: cel:CELE_W02A2.7

STRING: 6239.W02A2.7.2

UniGene: Cel.23008

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