ABI2 Recombinant Monoclonal Antibody

ABI2, or Abelson interactor 2, serves as a critical adaptor protein that bridges Abl family kinases to actin cytoskeleton remodeling machinery. This protein plays essential roles in regulating cell migration, membrane protrusion, and receptor endocytosis, making it a significant target in cancer research where aberrant cell motility contributes to metastatic progression. Understanding ABI2 function and expression patterns provides valuable insights into the molecular mechanisms underlying tumor cell invasion and spread.

This recombinant monoclonal antibody, clone 12F4, offers researchers the reproducibility and consistency that recombinant technology provides. Because the antibody sequence is defined and produced from a stable expression system, you can expect reliable performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations. The rabbit IgG format, purified by affinity chromatography, ensures high specificity for your target.

Validation studies demonstrate this antibody's utility in cellular localization and expression analysis workflows. Immunofluorescence staining of MCF-7 breast cancer cells reveals clear cytoplasmic signal when used at dilutions between 1:50 and 1:200, with successful visualization using standard fixation and permeabilization protocols. Flow cytometry analysis in SH-SY5Y neuroblastoma cells shows distinct positive population shifts compared to isotype controls, confirming the antibody's suitability for quantitative single-cell expression studies. These validated cell lines represent important models in cancer biology research.

For investigators studying actin dynamics, cell migration, or Abl kinase signaling networks in cancer contexts, this antibody provides a dependable tool for characterizing ABI2 expression and localization in human samples across immunofluorescence and flow cytometry applications.