ACTA2&ACTC1&ACTG2 Recombinant Monoclonal Antibody

Smooth muscle and cardiac actins represent essential cytoskeletal proteins that serve as fundamental markers for studying muscle differentiation, cardiovascular development, and smooth muscle biology. ACTA2 (alpha-smooth muscle actin) is widely recognized as a definitive marker for myofibroblasts and vascular smooth muscle cells, while ACTC1 (alpha-cardiac actin) plays a critical role in cardiac muscle contraction. ACTG2 (gamma-enteric smooth muscle actin) is predominantly expressed in visceral smooth muscle tissues. Together, these actin isoforms are indispensable tools for researchers investigating tissue remodeling, fibrosis, cardiac function, and smooth muscle pathologies.

This recombinant rabbit monoclonal antibody, clone 12C4, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations, ensuring your results remain comparable across experiments.

Validation data demonstrates robust performance across multiple platforms. Western blot analysis reveals clean detection at the expected 42 kDa molecular weight across diverse human cell lines including HeLa, HepG2, MCF-7, A431, COLO-205, Jurkat, and HEK293, with cross-species reactivity confirmed in mouse and rat heart tissue lysates. Immunohistochemistry staining in human skeletal muscle tissue shows specific localization, while immunofluorescence in HeLa cells and flow cytometry analysis further confirm reliable detection in cell-based assays.

This antibody serves researchers studying cardiovascular disease, fibrotic conditions, cancer-associated myofibroblasts, and smooth muscle disorders, providing a versatile detection tool validated across tissue sections, cultured cells, and flow cytometry applications.