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The Binding Activity of Human ACVR2A with Anti-ACVR2A&ACVR2B recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ACVR2A（CSB-MP001260HU1）at 2 μg/mL can bind Anti-ACVR2A&ACVR2B recombinant antibody . The EC50 is 3.848-4.375 ng/mL.
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The Binding Activity of Mouse ACVR2A with Anti-ACVR2A&ACVR2B recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse ACVR2A（CSB-MP001260MO1） at 2 μg/mL can bind Anti-ACVR2A&ACVR2B recombinant antibody. The EC50 is 1.562-1.966 ng/mL.
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The Binding Activity of Mouse Acvr2b with Anti-ACVR2A&ACVR2B recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Acvr2b（CSB-MP001261MO1） at 2 μg/mL can bind Anti-ACVR2A&ACVR2B recombinant antibody. The EC50 is 3.560-4.235 ng/mL.
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The Binding Activity of Human ACVR2B with Anti-ACVR2A&ACVR2B recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ACVR2B（CSB-MP623829HU） at 2 μg/mL can bind Anti-ACVR2A&ACVR2B recombinant antibody. The EC50 is 2.983-3.438 ng/mL.
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IHC image of CSB-RA623829MA1HU diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
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IHC image of CSB-RA623829MA1HU diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Immunofluorescence staining of SH-SY5Y cell with CSB-RA623829MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).
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The purity of ACVR2A&ACVR2B was greater than 95% as determined by SEC-HPLC
