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AGBL2 Recombinant Monoclonal Antibody

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Datasheet
Code CSB-RA732978MA1HU
Size $49.9
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Image
  • IHC image of CSB-RA732978MA1HU diluted at 1：100 and staining in paraffin-embedded mouse testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA732978MA1HU diluted at 1：100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • Overlay Peak curve showing jurkat cells stained with CSB-RA732978MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Q5U5Z8
Target Names
AGBL2
Alternative Names
AGBL 2 antibody; AGBL2 antibody; ATP/GTP binding protein like 2 antibody; ATP/GTP-binding protein-like 2 antibody; Carboxypeptidase 2 cytosolic antibody; CBPC2_HUMAN antibody; CCP2 antibody; Cytosolic carboxypeptidase 2 antibody; FLJ23598 antibody
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human AGBL2 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
hIgG1
Clone No.
1F3C12
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, FC
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
FC 1:50-1:200
Protocols
ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

AGBL2, also known as cytosolic carboxypeptidase 2 (CCP2), plays a critical role in the post-translational modification of tubulin through its deglutamylation activity. This enzyme removes glutamate residues from polyglutamate side chains on tubulin, directly influencing microtubule dynamics and stability. Understanding AGBL2 function has become increasingly relevant in research exploring neurodegeneration, ciliopathies, and cellular processes dependent on precise tubulin modification patterns.

This recombinant monoclonal antibody against AGBL2 offers the reproducibility that demanding research protocols require. Generated through recombinant technology and derived from a single clone (1F3C12), it provides sequence-defined consistency across lots, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The human IgG1 isotype format and affinity chromatography purification ensure reliable performance across experimental workflows.

Validation studies demonstrate versatility across multiple applications. Immunohistochemistry testing at 1:100 dilution has confirmed specific staining in both mouse testis tissue and human breast cancer sections using citrate buffer antigen retrieval on the Leica Bond system. Flow cytometry analysis of Jurkat cells shows clear positive signal separation from isotype control when used at 1:100 dilution with standard fixation and permeabilization protocols. This cross-species reactivity with human and mouse samples provides flexibility for researchers working across model systems.

Whether investigating tubulin modification in reproductive biology, exploring AGBL2 expression patterns in cancer contexts, or characterizing cytoskeletal regulation in immune cell populations, this antibody delivers the technical consistency needed for meaningful experimental outcomes. The validated performance in tissue sections and suspension cells makes it well-suited for researchers studying AGBL2 across diverse biological questions.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of tubulin protein. Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate. Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of non-tubulin proteins such as MYLK.
Gene References into Functions
  1. Findings suggest that ATP/GTP binding protein like 2 (AGBL2) plays a critical oncogenic role in the pathogenesis of hepatocellular carcinoma (HCC) through modulation on immunity-related GTPase family, M protein (IRGM)-regulated autophagy and aurora kinase A (Aurora A) activity. PMID: 29126912
  2. Suggest role for AGBL2 in cell proliferation and chemotherapy resistance in gastric cancer. PMID: 25916089
  3. High AGBL2 expression is associated with breast cancer. PMID: 24884516
  4. RARRES1, its interacting partners AGBL2, Eg5/KIF11, another EEY-bearing protein (EB1), and the microtubule tyrosination cycle are important in tumorigenesis. PMID: 21303978
Subcellular Location
Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, cilium basal body.
Protein Families
Peptidase M14 family
Database Links

HGNC: 26296

OMIM: 617345

KEGG: hsa:79841

STRING: 9606.ENSP00000298861

UniGene: Hs.147377

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