AP1G1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA065544A0HU
Size	US$210
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Image	Flow cytometric analysis of AP1G1 expression in HepG2 cells using AP1G1 antibody. Green, isotype control; red, AP1G1. Immunocytochemical staining of HepG2 cells with AP1G1 antibody. Nuclei were stained blue with DAPI; AP1G1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. Western blotting analysis using AP1G1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AP1G1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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Product Details
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Target Background

Product Details

Uniprot No.

O43747

Target Names

AP1G1

Alternative Names

AP1G1; Adaptor Related Protein Complex 1 Subunit Gamma 1; Gamma1-Adaptin; CLAPG1; ADTG; Clathrin Assembly Protein Complex 1 Gamma-1 Large Chain; Adaptor Related Protein Complex 1 Gamma 1 Subunit; Golgi Adaptor HA1/AP1 Adaptin Subunit Gamma-1; Adaptor Protein Complex AP-1 Subunit Gamma-1; AP-1 Complex Subunit Gamma-1; Clathrin-Associated/Assembly/Adaptor Protein, Large, Gamma 1; Clathrin Assembly Protein Complex 1 Gamma Large Chain; Adaptor-Related Protein Complex 1, Gamma 1 Subunit; Adapter-Related Protein Complex 1 Subunit Gamma-1; Adaptor-Related Protein Complex 1 Subunit Gamma-1; Golgi Adaptor HA1/AP1 Adaptin Gamma Subunit; Testicular Tissue Protein Li 21; Gamma Adaptin; USRISD

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human AP1G1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14F12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, FC, ICC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. In association with AFTPH/aftiphilin in the aftiphilin/p200/gamma-synergin complex, involved in the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes.

Gene References into Functions

Data indicate a functional link between adaptor protein complex 1 (AP-1) and the WD repeat protein 11 (WDR11) complex. PMID: 29426865
High ASCT2 expression is associated with drug resistance head and neck squamous cell carcinoma. PMID: 28823958
Depletion of the gamma2 or mu1A (AP1M1) subunits of AP-1, but not of gamma1 (AP1G1), precludes Nef-mediated lysosomal degradation of CD4. PMID: 27909244
Interaction between Rab4b and AP1gamma allows the budding of clathrin-coated vesicles for subsequent traffic to recycling endosomes. PMID: 24006255
Gamma-adaptin interacts directly with Rabaptin-5 through its ear domain.The interaction may play a role in membrane trafficking between the trans-Golgi network and endosomes. PMID: 11872161
crystal structure of the human gamma 1-adaptin ear domain consists solely of an immunoglobulin-like fold, unlike the alpha-adaptin ear domain PMID: 12042876
Gamma-adaptin, a novel ubiquitin-interacting adaptor, and Nedd4 ubiquitin ligase control hepatitis B virus maturation PMID: 16867982

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Subcellular Location

Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Cytoplasm, perinuclear region. Cytoplasmic vesicle, clathrin-coated vesicle.

Protein Families

Adaptor complexes large subunit family

Tissue Specificity

Widely expressed.

Database Links

HGNC: 555

OMIM: 603533

KEGG: hsa:164

STRING: 9606.ENSP00000377148

UniGene: Hs.461253

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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