Product Details

Uniprot No.

Q7Z3C6

Target Names

ATG9A

Alternative Names

Autophagy-related protein 9A (APG9-like 1) (mATG9), ATG9A, APG9L1

Species Reactivity

Human

Immunogen

A synthesized peptide from human ATG9A protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7G12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ATG9A serves as the only multi-spanning transmembrane protein in the core autophagy machinery, playing an essential role in autophagosome formation by delivering membrane to expanding phagophores. This unique trafficking protein cycles between the trans-Golgi network and endosomes, making it a critical marker for researchers investigating autophagy initiation, membrane dynamics, and cellular stress responses.

This recombinant monoclonal antibody, clone 7G12, offers the reproducibility that autophagy research demands. Generated through recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human ATG9A, provides consistent performance across your experimental timeline.

Validation in flow cytometry demonstrates clear detection of ATG9A in HepG2 human hepatocellular carcinoma cells. Using fixed and permeabilized cells with a 1:100 dilution, the antibody produces a distinct positive shift compared to isotype control, confirming reliable intracellular staining. This hepatocyte-derived cell line represents a particularly relevant model given the liver's high autophagic activity and the importance of autophagy in hepatic metabolism and disease. The validated protocol, employing 4% formaldehyde fixation and 0.2% Triton X-100 permeabilization, provides a solid starting point for your own flow cytometry workflows, with recommended dilutions ranging from 1:50 to 1:200 allowing optimization flexibility.

Whether you are characterizing autophagy flux, investigating ATG9A trafficking patterns, or exploring connections between autophagy and metabolic disease, this antibody supports quantitative single-cell analysis through flow cytometry and detection via ELISA, enabling diverse experimental approaches to study this fundamental cellular pathway.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. Also required to supply phosphatidylinositol 4-phosphate to the autophagosome initiation site by recruiting the phosphatidylinositol 4-kinase beta (PI4KB) in a process dependent on ARFIP2, but not ARFIP1. In addition to autophagy, also plays a role in necrotic cell death.

Gene References into Functions

the nucleation of autophagosomes occurs in endoplasmic reticulum ubulovesicular regions, where the ULK1 complex coalesces with ER and the ATG9 compartment PMID: 27510922
the identification of ATG9A as a specific AP-4 cargo PMID: 29180427
In the inner ear HEI-OC1 cell line, miR-34a overexpression resulted in an accumulation of phagophores and impaired autophagosome-lysosome fusion, and led to cell death subsequently. Notably, autophagy-related protein 9A (ATG9A), an autophagy protein, was significantly decreased after miR-34a overexpression. Knockdown of ATG9A inhibited autophagy flux, which is similar to the effects of miR-34a overexpression PMID: 28981097
Contrary to its normal trafficking between plasma membrane, intracellular organelles and autophagic membranes, ATG9A concentrates in transferrin receptor-positive juxtanuclear recycling endosomes in SMPD1-deficient cells. PMID: 27070082
Findings show for this first time that ATG9A loss in trastuzumab resistant cells allowed Her2 to escape from lysosomal targeted degradation through K63 poly-ubiquitination via c-Cbl. PMID: 27050377
phosphorylation of mATG9 at Tyr8 by Src and at Ser14 by ULK1 functionally cooperate to promote interactions between mATG9 and the AP1/2 complex. PMID: 27934868
In these Rab1B-depleted cells, ATG9A accumulated in intermediate membrane structures at autophagosome formation sites. These results indicate that Rab1B is involved in regulating the proper development of autophagosomes. PMID: 28522593
the C-terminal glycine of human ATG9A is required for its transport from the endoplasmic reticulum to the Golgi apparatus PMID: 27663665
these findings provide new insights into the intracellular pathways followed by ATG9A to reach different subcellular compartments, and into the intramolecular determinants that drive the sorting of this protein. PMID: 27316455
Results suggest that quinocetone stimulates the MRLC-mediated mAtg9 trafficking, which is critical for autophagosome formation, via the ATF6 upregulated expression of DAPK1. PMID: 27085326
Importantly, TBC1D14 and TRAPPIII regulate ATG9 trafficking independently of ULK1. PMID: 26711178
Data found that the interaction between 14-3-3 zeta and Atg9A is mediated by phosphorylation at Ser761. PMID: 25266655
TBC1D5 and the AP2 complex are important novel regulators of the rerouting of ATG9-containing vesicular carriers toward sites of autophagosome formation. PMID: 24603492
miR-29b mRNA, MAPK10 protein expression, and ATG9A protein expression are closely related to chemosensitivity of ovarian carcinoma. PMID: 24767251
the presence of ATG9A in the cytoplasm of tumor cells may be an independent biomarker for disease recurrence and survival in patients with oral squamous cell carcinoma PMID: 24085552
These findings suggest that Bif-1 acts as a critical regulator of Atg9 puncta formation presumably by mediating Golgi fission for autophagosome biogenesis during starvation. PMID: 21068542
mAtg9 trafficking through multiple organelles, including recycling endosomes, is essential for the initiation and progression of autophagy PMID: 22456507
ATG9A was found by co-immunoprecipitation to interact with FAM48A/p38IP/q8NEM7.2. PMID: 19893488
First demonstration that mammalian Atg9A, originally called mAtg9, is required for starvation-induced autophagy, and localizes to Golgi and endosomes. PMID: 16940348
In human adult tissues APG9L1 is ubiquitously expressed. PMID: 15755735

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Subcellular Location

Preautophagosomal structure membrane; Multi-pass membrane protein. Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

ATG9 family

Database Links

HGNC: 22408

OMIM: 612204

KEGG: hsa:79065

STRING: 9606.ENSP00000355173

UniGene: Hs.323363

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Code	CSB-RA441570A0HU
Size	US$210
	Order now
Image	Overlay Peak curve showing HepG2 cells stained with CSB-RA441570A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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ATG9A Recombinant Monoclonal Antibody

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