ATP6V1A Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA193957A0HU
Size	US$210
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Image	Flow cytometric analysis of ATP6V1A expression in HepG2 cells using ATP6V1A antibody. Green, isotype control; red, ATP6V1A. Immunocytochemical staining of HepG2 cells with ATP6V1A antibody. Nuclei were stained blue with DAPI; ATP6V1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using ATP6V1A antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm. Western blotting analysis using ATP6V1A antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP6V1A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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Target Background

Product Details

Uniprot No.

P38606

Target Names

ATP6V1A

Alternative Names

ATP6V1A; ATPase H+ Transporting V1 Subunit A; V-ATPase Subunit A; ATP6V1A1; ATP6A1; Vma1; VA68; VPP2; ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A; V-Type Proton ATPase (V-ATPase) Catalytic Subunit A; V-Type Proton ATPase Catalytic Subunit A; Vacuolar Proton Pump Subunit Alpha; ATPase, H+ Transporting, Lysosomal (Vacuolar Proton Pump), Alpha Polypeptide, 70kD, Isoform 1; H+-Transporting ATPase Chain A, Vacuolar (VA68 Type); ATPase, H+ Transporting, Lysosomal, Subunit A1; H(+)-Transporting Two-Sector ATPase, Subunit A; Vacuolar Proton Pump Alpha Subunit 1; Vacuolar ATPase Isoform VA68; V-ATPase 69 KDa Subunit 1; Vacuolar-Type H(+)-ATPase; V-ATPase 69 KDa Subunit; V-ATPase A Subunit 1; EC 3.6.3.14; EC 7.1.2.2; EC 3.6.3; ARCL2D; IECEE3; DEE93; HO68

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human ATP6V1A protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2D3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, FC, ICC, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000
IHC	1:100-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.; (Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction.

Gene References into Functions

We report biallelic mutations in ATP6V1E1 and ATP6V1A, respectively encoding the E1 and A subunits of the V1 domain of V-ATPase, as a cause of distinct metabolic and multisystemic cutis laxa entities. PMID: 28065471
V1A subunit of V-ATPase has a role in the progression and prognosis of gastric cancer. PMID: 25652905
Data show that the cAMP/PKA/CREB signaling pathway initiates acidosis-induced V-ATPase trafficking in salivary ducts via regulation of Rab11b expression. PMID: 22561749
These data introduce Rab11b as a crucial regulator and Rip11 as mediator of acidosis-induced V-ATPase traffic in duct cells of submandibular gland. PMID: 20717956
there is an important role for physical association between aldolase and the A, B and E subunits of V-ATPase in the regulation of the proton pump PMID: 17576770

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Involvement in disease

Cutis laxa, autosomal recessive, 2D (ARCL2D)

Subcellular Location

Cytoplasm. Cytoplasmic vesicle, secretory vesicle.

Protein Families

ATPase alpha/beta chains family

Tissue Specificity

High expression in the skin.

Database Links

HGNC: 851

OMIM: 607027

KEGG: hsa:523

STRING: 9606.ENSP00000273398

UniGene: Hs.477155

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

mTOR signaling pathway Oxidative Phosphorylation Synaptic vesicle cycle

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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