Code | CSB-RA223479A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:20-1:200 |
The AURKA recombinant monoclonal antibody is created using protein and DNA recombinant technologies. The first step involves immunizing mice with a synthesized peptide derived from human AURKA. Next, spleen cells are removed under sterile conditions, and total RNA is extracted from the spleen cells. The cDNA obtained from RNA reverse transcription is then used as a template for PCR amplification of the AURKA antibody gene. The obtained AURKA antibody gene is then introduced into a vector and transfected into host cells for cultivation. Finally, the AURKA recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. It undergoes rigorous verification and can be used for human AURKA protein detection in ELISA and IF experiments.
The AURKA protein is a serine/threonine kinase that plays an important role in cell division, specifically in the formation and regulation of the mitotic spindle. It is involved in centrosome maturation, separation of duplicated centrosomes, spindle assembly, chromosome alignment, and cytokinesis. AURKA activity is tightly regulated throughout the cell cycle by multiple mechanisms, including interactions with other proteins, phosphorylation, and localization to different cellular compartments. Dysregulation of AURKA activity has been implicated in various diseases, including cancer.
Applications : Western blot
Sample type: Human Cells
Review: Western blot assays showed that the tBHP and SAHA-induced suppression of phospho-FOXM1, AURKA and PLK1, as well as phospho-CCNB1.
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