Code | CSB-RA547773A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
IF | 1:50-1:200 |
FC | 1:50-1:200 |
The acetyl-HIST1H3A recombinant monoclonal antibody expression typically involves the initial step of inserting the HIST1H3A antibody-encoding gene into expression vectors. These vectors are then delivered into host cells via polyethyleneimine-mediated transfection. Culturing of the host cells leads to the production and secretion of the antibodies. Post-affinity chromatography purification, the antibodies' functionality is assessed through ELISA, IHC, IF, and FC tests, confirming their capacity to recognize the human acetylated HIST1H3A protein.
Acetylated HIST1H3A serves as a key epigenetic mark associated with the activation of gene expression. It modulates chromatin structure, making genes more accessible for transcription. This process plays crucial roles in various cellular processes, including development, differentiation, and the response to environmental cues.
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