BRAT1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA207827A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), HEK293 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), THP-1 whole cell lysate(30µg) All lanes: BRAT1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time：1min IHC image of CSB-RA207827A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA207827A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of PC-3 cell with CSB-RA207827A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA207827A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q6PJG6

Target Names

BRAT1

Alternative Names

BAAT 1 antibody; BAAT1 antibody; BRAT 1 antibody; brat1 antibody; BRAT1_HUMAN antibody; BRCA1-associated ATM activator 1 antibody; BRCA1-associated protein required for ATM activation protein 1 antibody; BRCA1-associated protein required for ATM activation-1 antibody; C7orf27 antibody; chromosome 7 open reading frame 27 antibody; HEAT repeat containing protein C7orf27 antibody; MGC22916 antibody; RMFSL antibody

Species Reactivity

Human, Rat

Immunogen

A synthesized peptide from human BRAT1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6H5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

BRAT1, or BRCA1-associated ATM activator 1, plays a critical role in the DNA damage response pathway by facilitating ATM kinase activation following genotoxic stress. This protein serves as an essential link between BRCA1 tumor suppressor function and the broader DNA repair machinery, making it a valuable target for researchers investigating genomic stability, cancer biology, and cellular stress responses.

This recombinant monoclonal antibody, clone 6H5, offers the reproducibility and consistency that demanding experimental workflows require. Because it is generated from a defined sequence rather than traditional hybridoma methods, researchers can expect uniform performance across different lots, eliminating a common source of experimental variability that can complicate long-term studies or multi-site collaborations.

Extensive validation demonstrates this antibody's versatility across multiple detection platforms. Western blot analysis confirms specific detection of BRAT1 at the expected 88 kDa molecular weight across a diverse panel of human cell lines, including HeLa, HEK293, MCF-7, SH-SY5Y, COLO-205, and THP-1 cells, providing confidence when working with various cellular models. Immunohistochemistry staining has been successfully performed on paraffin-embedded human kidney tissue and colorectal cancer specimens, supporting tissue-based investigations. The antibody also performs reliably in immunofluorescence applications, as demonstrated in PC-3 prostate cancer cells, and flow cytometry analysis validated in HeLa cells enables quantitative single-cell studies.

With confirmed reactivity in human and rat samples, this antibody supports comparative studies across species. Whether investigating epigenetic regulation, nuclear signaling cascades, or DNA damage response mechanisms, this BRAT1 antibody provides researchers with a dependable tool for advancing their understanding of this important pathway component.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress. Plays a role in regulating mitochondrial function and cell proliferation. Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors.

Gene References into Functions

Biallelic sequence variants in BRAT1 have been reported to cause a variety of ocular and systemic manifestations, but to our knowledge, this is the first report of inner retinal dysfunction manifest as selective loss of full-field ERG scotopic and photopic b-wave amplitudes. PMID: 28635423
compared with RMFSL, BRAT1 mutations can result in both moderately severe presentations evident by later-onset epilepsy and survival past infancy, as well as milder presentations that include intellectual disability, ataxia/dyspraxia, and cerebellar atrophy. PMID: 27282546
our data expand the clinical symptoms and demonstrate variability in the natural clinical course of BRAT1-associated phenotypes. Patients with early onset seizures, postnatal microcephaly, feeding problems, and muscular hypertonia or contractures should hence be screened for BRAT1 mutations. PMID: 27282648
we report two affected siblings with compound heterozygous truncating mutations in BRAT1 and intra-familial phenotypic heterogeneity, with a less severe disease course in the female sibling. This phenotypic variability should be taken into account when treating patients with BRAT1-associated neurodegenerative disease. PMID: 27480663
We conclude that BRAT1 should be added to the growing list of genes related to early-onset severe encephalopathy with epilepsy. PMID: 26535877
Our results further support that mutations of BRAT1 could lead to epileptic encephalopathy. PMID: 25319849
Ndfip1 is required during stress for ubiquitinating and trafficking BRAT1 into the nucleus. PMID: 25631046
Findings suggest novel roles of BRAT1 in cell proliferation and mitochondrial functions. PMID: 25070371
Data on crystal structure of BRCA1 binding with phosphopeptides suggest that C-terminal domain of BRCA1 interacts with BAAT1 and ATRIP with preferences for specific side chains; in BAAT1, phospho-Ser269 and Phe272 are the main interacting residues. PMID: 24073851
Study identified sequence variants in the known disease-causing genes SLC6A3 and FLVCR1, and present evidence to strongly support the pathogenicity of variants identified in TUBGCP6, BRAT1, SNIP1, CRADD, and HARS. PMID: 22279524

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Involvement in disease

Rigidity and multifocal seizure syndrome, lethal neonatal (RMFSL)

Subcellular Location

Nucleus. Cytoplasm.

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 21701

OMIM: 614498

KEGG: hsa:221927

STRING: 9606.ENSP00000339637

UniGene: Hs.520623

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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