CAMK2G&CAMK2B Recombinant Monoclonal Antibody

Calcium/calmodulin-dependent protein kinase II (CaMKII) represents a critical hub in cellular signaling, with the beta and gamma subunits playing essential roles in synaptic plasticity, learning, memory formation, and calcium-mediated signal transduction. These kinases are particularly abundant in neural tissue but also contribute to signaling cascades in diverse cell types, making them valuable targets for neuroscience research, cancer biology, and studies of calcium-dependent cellular processes.

This recombinant monoclonal antibody, generated against a synthesized peptide from human CAMK2G and CAMK2B, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations, ensuring your results remain comparable across experiments.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis confirms detection in human cell lines including MCF-7, SH-SY5Y, PC-3, HT-29, and HeLa, as well as mouse and rat brain tissue lysates, providing cross-species reactivity for translational studies. The observed band at approximately 55 kDa, rather than the predicted 73 kDa, likely reflects post-translational processing or the detection of specific isoform variants common to CaMKII subunits. Immunohistochemistry staining has been validated in human colorectal cancer and kidney tissue sections, while immunofluorescence and flow cytometry applications have been confirmed in MCF-7 and PC-3 cells respectively.

Whether investigating neuronal signaling mechanisms, exploring CaMKII dysregulation in cancer, or examining calcium-dependent pathways in various disease models, this antibody provides a reliable foundation for your research.