CAV2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA789994A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: A549 whole cell lysate(30µg), HEK293T whole cell lysate(30µg), Hela whole cell lysate(30µg), A431 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), U-251MG whole cell lysate(30µg) All lanes: Caveolin 2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time：40s IHC image of CSB-RA789994A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A431 cell with CSB-RA789994A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing 786-O cells stained with CSB-RA789994A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P51636

Target Names

CAV2

Alternative Names

Caveolin-2, CAV2

Species Reactivity

Human

Immunogen

A synthesized peptide from human CAV2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11D4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Caveolin-2 serves as a critical scaffolding protein within caveolae, the flask-shaped plasma membrane invaginations essential for signal transduction, lipid regulation, and endocytosis. Working alongside caveolin-1, CAV2 participates in organizing signaling complexes and has been implicated in cell cycle regulation, making it a valuable target for researchers investigating membrane dynamics, cancer biology, and cellular signaling pathways.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human CAV2, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can compromise longitudinal studies and ensures reliable performance across your research timeline.

Validation across multiple detection platforms demonstrates this antibody's versatility in your experimental toolkit. Western blot analysis confirms specific detection of CAV2 at the expected 18 kDa molecular weight across diverse human cell lines including A549, HEK293T, HeLa, A431, MCF-7, and U-251MG, providing confidence when working with various cellular models. Immunohistochemistry testing in paraffin-embedded human pancreatic cancer tissue reveals clear staining patterns suitable for tissue-based investigations. Immunofluorescence studies in A431 cells and flow cytometry analysis using 786-O cells further extend its utility for subcellular localization studies and quantitative single-cell analysis.

Whether you are exploring caveolae-mediated endocytosis, investigating CAV2's role in tumor progression, or examining membrane microdomain organization, this antibody provides the specificity and application flexibility needed to advance your research with confidence.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.

Gene References into Functions

Cav-2beta isoform yielded by alternative translation initiation desensitizes insulin receptor (IR) via dephosphorylation by PTP1B, and subsequent endocytosis and lysosomal degradation of IR, causing insulin resistance. PMID: 29604334
Results verified the presence of transcript III of cav-2 for the first time, but no protein associated was found. Also, a decreasing trend of cav-2 (transcripts I and II) were observed in tumoral tissues especially in stages I and II and seem to be associated with the incidence and promotion of breast cancer, especially in the initial stages of breast cancer. PMID: 28857238
High CAV2 expression is associated with lung cancer. PMID: 26930711
A-type lamin-dependent Caveolin-2 homo-oligomerization in the inner nuclear membrane microdomain is a precondition for pY19-Caveolin-2-mediated insulin-response epigenetic activation at the nuclear periphery. PMID: 27552914
Caveolin-2 expression is necessary for the control of E2-dependent cellular proliferation of MCF-7 cells. PMID: 26480297
variant in CAV2 is associated with increased age-of-onset of P. aeruginosa airway infection in cystic fibrosis cohort. PMID: 26047157
Results present initial characterization of key proteins Cav2 and CFL1 as cellular factors that colocalize with M in viral inclusions and filaments and ZNF502 protein which appears to interact with RSV M in the nucleus. PMID: 25556234
Our findings did not correspond with previous positive results, suggesting that CAV1-CAV2 variants studied in the present study are not important risk factors for Normal Tension Glaucoma. PMID: 23743525
This study does not support an association between CAV2 variation and kidney transplant survival. PMID: 23667606
The combination of the expression of the genes for PPARgamma, STMN1 and CAV2 was significantly predictive for early recurrence of non-muscle-invasive bladder cancer PMID: 21489836
In contrast to wild-type-Cav-2, retroviral re-expression of Y19/27F-Cav-2 in Cav-2 knockout endothelial cells did not affect anti-proliferative effect of TGF-beta compared to empty vector. PMID: 22819829
Stromal caveolin-2 expression were more frequent in anaplastic carcinoma and diffuse sclerosing variant of papillary carcinoma compared to conventional papillary thyroid carcinoma. PMID: 22236542
CAV1 mRNA and protein levels are reduced by both NFBD1 knockdown and knockout independently of IR and p53 PMID: 21551225
cav-2 acts as a modulator of cancer progression. PMID: 21373752
We found preliminary evidence that CAV2 rs2270188 interacts with dietary fat to affect risk of type 2 diabetes. PMID: 21178094
Single Nucleotide Polymorphisms in CAV2 is associated with primary open-angle glaucoma. PMID: 20835238
In Caco-2 cells, a polarized cell line derived from human colon cancer that does not express caveolin 1 (Cav-1), there was no detectable expression of caveolin 2 (Cav-2). PMID: 12414992
caveolin-2 may play a role in lamellar granule assembly, trafficking, and/or function. PMID: 12648214
PPARgamma ligands increased the levels of caveolin-2 2-5-fold in a concentration-dependent manner within 24 h. Nonthiazolidinedione PPARgamma ligands elevated caveolin-2 protein 3-4-fold. PMID: 12813462
New evidence demonstrating that Cav-2 undergoes phosphorylation at both tyrosines 19 and 27 also reveals that tyrosine phosphorylation of Cav-2 has no effect on its targeting to lipid rafts, but clearly disrupts hetero-oligomerization of Cav-2 with Cav-1. PMID: 15504032
Heterologously and endogenously expressed D1 dopamine receptors in renal cells are associated with and regulated by caveolin-2. PMID: 15569306
Overexpression of caveolin-2 is associated with inflammatory breast cancer PMID: 16244790
CAV2 identified and immunolocalized in the caveola-vesicle complexes (CVC )present in erythrocytes infected with P. vivax PMID: 16521037
Caveolin-2 was expressed in the sinusoidal endothelial cells and the smooth muscle cells of the unparied arteries of hepatpcellular carcinoma specimens. PMID: 17898556
CAV2 is preferentially expressed in basal-like cancers and is associated with poor prognosis PMID: 17912630
Data suggests a possible involvement of serine 36-phosphorylated caveolin-2 in modulating mitosis. PMID: 18081315

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Subcellular Location

Nucleus. Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein.

Protein Families

Caveolin family

Tissue Specificity

Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.

Database Links

HGNC: 1528

OMIM: 601048

KEGG: hsa:858

STRING: 9606.ENSP00000222693

UniGene: Hs.212332

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Focal adhesion Endocytosis

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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