CDC40 Recombinant Monoclonal Antibody

Code CSB-RA910400A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate(30µg), HL-60 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), Hela whole cell lysate(30µg), HEK293T whole cell lysate(30µg), THP-1 whole cell lysate(30µg), SP2/0 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg)
    All lanes: CDC40 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 66 kDa
    Observed band size: 74 kDa
    Exposure time:40s
  • IHC image of CSB-RA910400A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details

Uniprot No.
Target Names
CDC40
Alternative Names
Pre-mRNA-processing factor 17 (Cell division cycle 40 homolog) (EH-binding protein 3) (Ehb3) (PRP17 homolog) (hPRP17), CDC40, EHB3 PRP17 PRPF17
Species Reactivity
Human, Mouse
Immunogen
A synthesized peptide from human CDC40 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
10C12
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Required for pre-mRNA splicing as component of the activated spliceosome.
Gene References into Functions
  1. HBx activates NF-kappaB to promote the expression of miR1269b, which augments CDC40 expression, contributing to malignancy in hepatocellular cancer. PMID: 27349221
Subcellular Location
Nucleus. Nucleus speckle.
Database Links

HGNC: 17350

OMIM: 605585

KEGG: hsa:51362

STRING: 9606.ENSP00000304370

UniGene: Hs.428147

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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