CLGN Recombinant Monoclonal Antibody

Calmegin (CLGN) is a testis-specific endoplasmic reticulum chaperone protein that plays an essential role in male fertility and sperm-egg fusion. As a member of the calnexin family, calmegin facilitates proper protein folding during spermatogenesis and is critical for the functional maturation of sperm surface proteins required for fertilization. This makes CLGN an important target for researchers investigating reproductive biology, male infertility mechanisms, and germ cell development.

This recombinant monoclonal antibody, clone 4C6, offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a sequence-defined rabbit IgG framework, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The defined clonal origin ensures that researchers can reproduce their results with confidence across experiments spanning months or years.

Raised against a synthetic peptide derived from human CLGN and purified by affinity chromatography, this antibody has been validated for flow cytometry applications with human samples. Flow cytometric analysis using HepG2 cells demonstrated clear positive signal separation from isotype control, with optimal performance observed at dilutions between 1:50 and 1:200. The validation protocol employed fixed and permeabilized cells, confirming the antibody's suitability for intracellular staining of this ER-resident protein. Additional validation for ELISA applications provides flexibility for researchers requiring quantitative detection methods.

This antibody serves as a valuable tool for investigators studying spermatogenesis, fertilization mechanisms, ER quality control pathways, and the molecular basis of male reproductive disorders.