CLPTM1 Recombinant Monoclonal Antibody

CLPTM1, or cleft lip and palate transmembrane protein 1, is a transmembrane protein that has garnered research interest for its potential roles in cellular processes and its association with craniofacial development. Understanding CLPTM1 expression patterns and localization provides valuable insights for researchers investigating developmental biology, membrane protein function, and related disease mechanisms.

This recombinant rabbit monoclonal antibody, clone 9E5, offers the consistency and reliability that demanding research workflows require. Because it is produced from a defined sequence rather than traditional hybridoma methods, you can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations. The antibody is raised against a synthesized peptide derived from human CLPTM1 and purified by affinity chromatography to ensure high specificity.

Validation testing demonstrates this antibody's versatility across multiple experimental platforms. Western blot analysis successfully detects CLPTM1 in MCF-7 and HeLa whole cell lysates at dilutions ranging from 1:500 to 1:2000, confirming reliable performance in standard protein expression studies. For tissue-based investigations, immunohistochemistry validation in paraffin-embedded human lung cancer tissue shows clear staining at 1:100 dilution using citrate buffer antigen retrieval. Immunofluorescence studies in A549 cells further demonstrate the antibody's utility for subcellular localization experiments, with effective staining observed at 1:50 dilution.

This antibody is well-suited for researchers exploring CLPTM1 biology in cancer cell models, developmental studies, or membrane protein characterization. Its compatibility with ELISA, western blot, immunohistochemistry, and immunofluorescence provides flexibility to address diverse experimental questions within a single research program.