CLTC/CLTCL1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA172477A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), K562 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), HL-60 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), HEK293 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), Rat brain tissue lysate(30µg) All lanes: Clathrin heavy chain 1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 192, 187 kDa Observed band size: 192 kDa Exposure time：10s IHC image of CSB-RA172477A0HU diluted at 1:100 and staining in paraffin-embedded mouse kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA172477A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing MCF-7 cells stained with CSB-RA172477A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
Customer Reviews and Q&A

Product Details

Uniprot No.

Q00610 P53675

Target Names

CLTC/CLTCL1

Alternative Names

CHC17 antibody; Clathrin heavy chain antibody; Clathrin heavy chain 1 antibody; Clathrin heavy chain 2 antibody; Clathrin heavy chain like 1 antibody; Clathrin heavy chain on chromosome 17 antibody; Clathrin heavy polypeptide antibody; CLH 17 antibody; CLH 22 antibody; CLH-17 antibody; CLH1_HUMAN antibody; CLH17 antibody; CLH22 antibody; CLTC antibody; CLTCL antibody; CLTCL1 antibody; CLTCL2 antibody; CLTD antibody; Hc antibody; KIAA0034 antibody; CLTCL1 antibody; CLH22 antibody; CLTCL antibody; CLTDClathrin heavy chain 2 antibody; Clathrin heavy chain on chromosome 22 antibody; CLH-22 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human CLTC/CLTCL1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15H3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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