CSDE1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA960273A0HU
Size	US$210
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Image	Immunofluorescence staining of MCF-7 cell with CSB-RA960273A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing MCF-7 cells stained with CSB-RA960273A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

O75534

Target Names

CSDE1

Alternative Names

Cold shock domain-containing protein E1 (N-ras upstream gene protein) (Protein UNR), CSDE1, D1S155E KIAA0885 NRU UNR

Species Reactivity

Human

Immunogen

A synthesized peptide from human CSDE1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11B11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CSDE1, also known as cold shock domain-containing protein E1 or protein UNR, functions as an RNA-binding protein that plays critical roles in translational regulation, mRNA stability, and cellular stress responses. Originally identified as an N-ras upstream gene product, CSDE1 has emerged as a significant factor in developmental biology, stem cell maintenance, and cancer progression, where its dysregulation can influence cell proliferation and differentiation pathways.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human CSDE1, offers the reproducibility and consistency that demanding research applications require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations. The rabbit IgG format, purified by affinity chromatography, ensures high specificity for your target while minimizing background interference.

Validation studies demonstrate reliable performance across multiple experimental platforms. Immunofluorescence analysis of MCF-7 human breast cancer cells reveals clear cytoplasmic staining patterns consistent with CSDE1's known localization as an RNA-binding protein, with optimal results achieved at dilutions between 1:50 and 1:200. Flow cytometry experiments using the same cell line confirm robust detection with excellent signal separation from isotype controls, supporting quantitative analysis of CSDE1 expression at the single-cell level.

This antibody serves researchers investigating post-transcriptional gene regulation, cellular stress mechanisms, and cancer biology. Its validated performance in both imaging and flow-based applications provides flexibility for studies examining CSDE1 expression patterns, subcellular distribution, or population-level expression heterogeneity in human cell models.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

RNA-binding protein involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Required for efficient formation of stress granules.; (Microbial infection) Required for internal initiation of translation of human rhinovirus RNA.

Gene References into Functions

CSDE1 post-transcriptionally modulates core components of multiple regulatory nodes of human embryonic stem cell identity, neuroectoderm commitment and neurogenesis and acts as a negative regulator of neural differentiation and neurogenesis. PMID: 29129916
UNR/CDSE1 expression is correlated with outcome in patients with resectable pancreatic ductal adenocarcinoma PMID: 28763470
Results identify UNR as an oncogenic modulator of melanoma progression, unraveling the underlying molecular mechanisms. UNR seems to regulate translation elongation of Vimentin and RAC1 mRNAs. PMID: 27908735
Studies indicate that Unr (upstream of N-ras) is post-translationally modified by phosphorylation and lysine acetylation. PMID: 26614667
Hepsin suppressed CDK11p58 internal ribosome entry site activity in prostate cancer cells by modulating UNR expression and eIF-2alpha phosphorylation. PMID: 25576733
Study suggests TRIM33 and NRAS-CSDE1 as candidate genes for autism, and may provide a novel insight into the etiology of autism PMID: 24189344
unr and nPTB act as RNA chaperones by changing the structure of the IRES into one that permits translation initiation PMID: 12667457
UNR translation is regulated by an internal ribosome entry site that is downregulated by polypyrimidine tract binding protein. PMID: 15928332
regulation of IRES-mediated translation by hnRNP C1/C2 and Unr might be important in mitosis PMID: 17159903
The data suggest that Unr acts as an RNA chaperone on rhinovirus RNA 5' UTR to maintain a complex tertiary internal ribosome entry site structure required for translational competency. PMID: 17947529

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Subcellular Location

Cytoplasm. Cytoplasm, Stress granule. Cytoplasm, P-body.

Database Links

HGNC: 29905

OMIM: 191510

KEGG: hsa:7812

STRING: 9606.ENSP00000407724

UniGene: Hs.69855

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Virus Infection Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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