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| Code | CSB-RA959139A0HU |
| Size | US$210 |
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| Application | Recommended Dilution |
|---|---|
| IHC | 1:50-1:200 |
| FC | 1:50-1:200 |
The production of the CSN2 recombinant monoclonal antibody involves a well-defined and rigorous process to ensure its quality and specificity. Initially, B cells are isolated from an immunized animal using the synthesized peptide derived from human CSN2 as the immunogen. Following that, total RNA is extracted from the isolated B cells and converted into cDNA through reverse transcription. The CSN2 antibody genes are amplified using PCR with specific primers designed for the antibody constant regions and inserted into an expression vector. This expression vector is then introduced into host cells, enabling the production of the CSN2 recombinant monoclonal antibody. The antibody is collected from the cell culture supernatant and undergoes purification using affinity chromatography, resulting in a highly purified form. Stringent characterization assays, including ELISA, IHC, and FC analysis, are performed to validate the antibody's specificity and functionality, ensuring its precise binding to human CSN2 protein.
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