CTSS Recombinant Monoclonal Antibody

Code CSB-RA788455A0HU
Size US$210
Order now
Image
  • IHC image of CSB-RA788455A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA788455A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Uniprot No.
Target Names
CTSS
Alternative Names
Cathepsin S (EC 3.4.22.27), CTSS
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human Cathepsin S
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
9B10
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

CTSS is an important lysosomal cysteine protease. Antigen presentation, cytokine production, and angiogenesis are all important functions of this protein. CTSS is an essential cysteine protease that plays a role in the pathogenesis of autoimmune illnesses, allergic inflammation, asthma, diabetes, obesity, cardiovascular and pulmonary diseases, and cancer by enhancing tumor invasiveness or acting as a biomarker for cardiovascular disease evaluation. CTSS activates the PI3K/Akt and MAPK pathways, which enhance tumor cell growth. CTSS can also stimulate the proliferation of human periodontal ligament cells.

The first step in the preparation of recombinant CTSS antibody is to obtain the CTSS antibody gene. The heavy and light chain genes of the antibody were constructed into a plasma vector and then transfected into suspended mammalian cells transiently. After expression verification, cell supernatant was collected in expanded culture and purified recombinant CTSS antibody was obtained using Affinity-chromatography. This recombinant CTSS antibody has been validated for the detection of CTSS protein from Human in the ELISA, IHC.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.
Gene References into Functions
  1. Plasma apoA-I proteolysis is augmented in aortic valve stenosis and cathepsin S exerts the most deleterious effects on apoA-I integrity in humans. PMID: 29915952
  2. The present data indicating that Cat-S activity increases with CKD progression suggest that Cat-S might be a therapeutic target to prevent cardiovascular complications in CKD. PMID: 28240259
  3. High expression of CTSS was independently associated with lymph node metastasis (OR, 2.015; 95% CI, 1.225-3.315; P=0.006). Therefore, CTSS may serve as a predictive risk marker for the progression and prognosis of papillary thyroid cancer . PMID: 29749483
  4. Authors show a significant increase of circulating CS levels in healthy female subjects induced by long-term physical activity. PMID: 29181829
  5. This study therefore considerably improves our understanding of the molecular mechanism responsible for cathepsin S inhibition and facilitates the identification of potential novel selective inhibitors of cathepsin S. PMID: 28236030
  6. cathepsin S is increased in periodontal cells and tissues under inflammatory and infectious conditions, suggesting a critical role of this autophagy-associated molecule in the pathogenesis of periodontitis. PMID: 29362520
  7. Elevated cathepsin S activity was associated with collagen I degradation and thus might be involved in the progression of abdominal aortic aneurysms. PMID: 29624112
  8. Cathepsin S was identified as the major IL-36gamma-activating protease expressed in epithelial cells. PMID: 28289191
  9. Our results indicate that autophagy is essential for decreasing CTSS activity to inhibit tumor metastasis by hispolon treatment in cervical cancer; this finding provides a new perspective on molecular regulation. PMID: 28981104
  10. These results revealed that CTSS can regulate EGFR signalling by facilitating EGF-mediated EGFR degradation. PMID: 27387133
  11. This study suggested serum Cat S may be a potential biomarker for the diagnosis and prognosis of gastric cancer PMID: 27058412
  12. Overall, these results indicate that exploitation of the cathepsin S activity in MPS tissues can be utilized to substantially lower non-target accumulation, suggesting this is a promising approach for the development of diagnostic and radiotherapeutic nanomedicine platforms. PMID: 27372424
  13. polymorphism of rs7534124 and rs1136774 in cathepsin S promoter may decrease the susceptibility of asthma in a Chinese Han population PMID: 27249607
  14. cathepsin S mRNA (CTSS), which encodes a cysteine protease associated with angiogenesis and atherosclerosis, is highly edited in human endothelial cells. RNA editing enables the recruitment of the stabilizing RNA-binding protein human antigen R (HuR; encoded by ELAVL1) to the 3' UTR of the CTSS transcript, thereby controlling CTSS mRNA stability and expression. PMID: 27595325
  15. the essential role of autophagy-regulated early ROS in triggering late apoptotic signaling PMID: 26029922
  16. Cathepsin S cleaves near the N-terminus of PAR2 to expose a novel tethered ligand, KVDGTS. PMID: 24964046
  17. Increased plasma CTSS concentration is associated with atherogenesis. PMID: 24727728
  18. potential marker of pruritus in dandruff/seborrhoeic dermatitis PMID: 24690038
  19. results identify Cat-S as a biased agonist of PAR2 that causes PAR2- and TRPV4-dependent inflammation and pain. PMID: 25118282
  20. Its stability at neutral pH and potent proteolytic activity on extracellular matrix components mean that cathepsin S may contribute significantly to cartilage degradation and may thus be considered a potential drug target in joint diseases. PMID: 23152404
  21. High cathepsin S expression at the primary site correlates with decreased brain metastasis-free survival in breast cancer patients. PMID: 25086747
  22. cathepsin S and chemerin only correlated positively with insulin resistance and inflammation PMID: 24390241
  23. Cat S-mediated autophagic flux is an important mechanism for inducing M2-type polarization of tumor-associated macrophages, which leads to tumor development PMID: 24580730
  24. Cathepsin S plays an important role in the regulation of autophagy and apoptosis in human glioblastoma cells. PMID: 24875536
  25. Suppression of CD47 by morpholino approach suppressed growth of hepatocellular carcinoma in vivo and exerted a chemosensitization effect through blockade of CTSS/PAR2 signaling. PMID: 24523067
  26. The miR-31/IRF-1/CTSS pathway may play a functional role in the pathogenesis of cystic fibrosis lung disease. PMID: 24940638
  27. Markedly high levels of tear CTSS activity are suggestive of Sjogren syndrome. PMID: 24644101
  28. This study reveals a noncanonical molecular pathway in which, after the inhibition of cathepsin S, autophagy induces early reactive oxygen species production for oxidative DNA damage and cell death through xanthine oxidase. PMID: 23892358
  29. Amyloid-beta can regulate the proinflammatory state of human macrophages by inducing MMP-2/MMP-9/cathepsin S levels and by reducing TGF-beta1 secretion. PMID: 24335416
  30. Cat S may participate in the pathophysiology of cystic fibrosis by weakening the antibacterial activity of SP-A. PMID: 23707200
  31. the genetic polymorphisms of CTSS were associated with metabolic disorders in a Chinese Han population PMID: 23747398
  32. Cathepsin S activity appears to be involved in the early dysregulation of glucose and insulin metabolism. PMID: 22923671
  33. Cleavage of nidogen-1 by cathepsin S impairs its binding to basement membrane partners PMID: 22952693
  34. The present study was designed to determine the role of Cat S in human hepatocellular carcinoma cell growth, invasion and angiogenesis, using RNA interference technology. PMID: 22796222
  35. CTSS variants seem to be nominally associated to obesity related traits and this association may be modified by dietary protein intake PMID: 22844403
  36. HCV has an inhibitory role on cathepsin S-mediated major histocompatibility complex (MHC) class II maturation, which may contribute to weak immunogenicity of viral antigens in chronic infections. PMID: 22761382
  37. Cathepsin S therefore appears to be an important factor that influences selection of autoreactive T cells. PMID: 22424724
  38. Cathepsin S specificity profile appears to be pH-independent. PMID: 21967108
  39. Cathepsin S plays an important role in the regulation of cell autophagy through interference with the EGFR-ERK/MAPK-signaling pathway. PMID: 22101325
  40. results signify that CatS may be an important prognostic biomarker and predictive of response to adjuvant fluoruracil/folinic acid in colorectal carcinoma PMID: 21989182
  41. The cathepsin S deserves further evaluation as therapeutic targets to develop disease modifying drugs to treat Alzheimer's disease. PMID: 21585286
  42. Serum cathepsin S showed significantly lower values 4 days after surgery PMID: 21250859
  43. Among elderly individuals in 2 independent cohorts, higher serum cathepsin S levels were associated with increased mortality risk PMID: 21878432
  44. results suggest that CTSS mediated atherogenesis may be associated with a CD40 mediated inflammatory and immune response. Further invasive atheroma analysis is reasonable PMID: 21223957
  45. Cathepsin S activity in human whole blood was dependent on the time of blood collection, suggesting that cathepsin S activity is subject to circadian variations. PMID: 21094118
  46. Our data provide first evidence that Cathepsin S expression is selectively induced in psoriatic keratinocytes. PMID: 19849712
  47. Down-regulation of cathepsin S was found in inverted papilloma tissues as compared with its expression level in normal sinus mucosa tissues. PMID: 21038029
  48. the immunosuppressive effects of IL-10-STAT3 on MHC-II antigen presentation may occur via the inhibition of cathepsin S expres PMID: 20356901
  49. The researchers found no evidence of an association between the CTSS -25G/A polymorphism and the presence of coronary artery disease in a Chinese population. PMID: 19593952
  50. cathepsin S was found to be significantly elevated in rheumatoid arthritis and osteoarthritis synovial fluid. PMID: 20180636

Show More

Hide All

Subcellular Location
Lysosome. Secreted. Cytoplasmic vesicle, phagosome.
Protein Families
Peptidase C1 family
Database Links

HGNC: 2545

OMIM: 116845

KEGG: hsa:1520

STRING: 9606.ENSP00000357981

UniGene: Hs.181301

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*