Code | CSB-RA554990A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
The production process for the CYP17A1 recombinant antibody consists of four main steps. First, the CYP17A1 monoclonal antibody gene is sequenced and cloned into a plasmid vector. The recombinant vector is then introduced into a host cell line, followed by the purification of the CYP17A1 recombinant monoclonal antibody from the cell culture supernatant using affinity chromatography. During the production of the CYP17A1 monoclonal antibody, a synthesized peptide derived from human CYP17A1 is used as the immunogen. This CYP17A1 recombinant monoclonal antibody is recommended for use in ELISA, WB, IHC, and IF applications to detect human and rat CYP17A1 proteins.
The CYP17A1 protein is an enzyme involved in steroid hormone biosynthesis. It plays a crucial role in the production of androgens (such as testosterone) and estrogens in the gonads and adrenal glands. Specifically, CYP17A1 catalyzes two sequential reactions in the steroidogenic pathway. The first reaction is the conversion of pregnenolone or progesterone to 17α-hydroxypregnenolone or 17α-hydroxyprogesterone, respectively, by its 17α-hydroxylase activity. The second reaction is the conversion of 17α-hydroxypregnenolone or 17α-hydroxyprogesterone to dehydroepiandrosterone (DHEA) or androstenedione, respectively, by its 17,20-lyase activity. These two reactions are essential for the synthesis of androgens, which are important for the development of male sex organs and secondary sexual characteristics. In addition, CYP17A1 also plays a role in the biosynthesis of estrogens, which are important for the development of female reproductive organs and secondary sexual characteristics.
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