Product Details

Uniprot No.

Target Names

CYP1A2

Alternative Names

Cytochrome P450 1A2 (EC 1.14.14.1) (CYPIA2) (Cholesterol 25-hydroxylase) (Cytochrome P(3)450) (Cytochrome P450 4) (Cytochrome P450-P3), CYP1A2

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human Cytochrome P450 1A2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8B2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Cytochrome P450 1A2 plays a central role in hepatic drug metabolism, catalyzing the oxidation of numerous xenobiotics, pharmaceuticals, and endogenous compounds. As a key enzyme in phase I metabolism, CYP1A2 influences the pharmacokinetics of clinically important drugs and participates in the bioactivation of procarcinogens, making it an essential target for researchers investigating drug-drug interactions, toxicology, and cancer susceptibility.

This recombinant monoclonal antibody offers the reproducibility that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, it eliminates the lot-to-lot variability inherent to traditional hybridoma-derived antibodies, ensuring consistent performance across long-term studies and multi-site collaborations. The rabbit IgG format, produced against a synthetic peptide derived from human Cytochrome P450 1A2, provides high specificity for your target.

Validation studies confirm robust performance across multiple applications. In western blot analysis, the antibody detects a band at the predicted molecular weight of 59 kDa in HeLa, HepG2, and L02 whole cell lysates, with effective dilutions ranging from 1:500 to 1:5000 offering flexibility to optimize signal intensity for your specific experimental conditions. For tissue-based studies, immunohistochemistry validation in paraffin-embedded human kidney tissue demonstrates clear staining at 1:50 to 1:200 dilutions using standard citrate buffer antigen retrieval, providing a reliable option for examining CYP1A2 expression patterns in clinical and research specimens.

Whether you are exploring metabolic enzyme regulation, investigating drug metabolism pathways, or examining CYP1A2 expression in cancer and cardiovascular disease contexts, this antibody delivers the consistency and validated performance needed to advance your research with confidence.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation (Probable).

Gene References into Functions

The in vitro studies of Pristimerin (PTM) with CYP isoforms indicate that PTM has the potential to cause pharmacokinetic drug interactions with other co-administered drugs metabolized by CYP1A2, 3A4 and 2C9. PMID: 28385095
Phenotyping of CYP 4501A2 Activity by Total Overnight Salivary Caffeine Assessment (TOSCA) in Patients on Warfarin Treatment PMID: 28992765
Both CYP1A2 and CYP2B4 activities were affected by their coexpression in a manner consistent with formation of the high-affinity POR-CYP1A2-CYP2B4 complex. PMID: 29233819
The influence of CYP1A2 genotype in the basal and post-caffeine blood pressure response to caffeine ingestion is modified by physical activity status and caffeine consumption level. PMID: 29522901
The metabolites are predominantly catalyzed by CYP3A4 (77% approximately 87%), with a moderate contribution from CYP3A5 (5% approximately 15%) and CYP1A2 (3.7% approximately 7.5%). PMID: 29247736
the genetic variations of the CYP1A2 gene did not affect the ergogenicity or side effects derived from the consumption of a moderate dose of caffeine. PMID: 29668752
The findings of this study indicate that CYP2C19 contribute to the metabolism of MDMA to MDA in humans PMID: 28117133
a positive association between caffeine intake and abstract reasoning exists in the CC homozygous of the -163C>A polymorphism of CYP1A2 gene. PMID: 29072170
CYP1A2 C164A polymorphism has an interaction with maternal caffeine intake that affects infant birth size in a Japanese cohort study PMID: 28355205
findings provide evidences that miR-320 might inhibit the development of Steroid-induced avascular necrosis of femoral head by targeting CYP1A2. PMID: 29551500
our findings suggest that CYP1A2, CYP3A4 and CYP3A5 play an important role in osteosarcoma tumorigenesis, at primary and metastatic sites, as well in treatment response PMID: 28404946
CYP1A2 rs2470890 was significantly associated with the prognosis of patients with breast cancer and could serve as an independent impact factor of prognosis of breast carcinoma PMID: 28418906
CYP1A2 activity can be monitored using noninvasive random urine samples, and it can be used to assess developmental changes in theophylline clearance by preterm PMID: 27995649
The increased risk of prostate cancer was observed among individuals with the CYP1A2 CA + AA genotype (OR, 1.43; 95% CI, 1.03-2.00). PMID: 29165164
Nerolidol induces endoplasmic reticulum stress and apoptosis in Human HepG2 Cells through extensive CYP2C19 and CYP1A2 mediated drug oxidation. PMID: 28256105
Findings show that at allelic and genotypic level polymorphisms in CYP3A4 and CYP1A2 are significantly associated with a reduced risk of drug addiction in Chinese individuals. PMID: 27257124
Results suggest that CYP4503A4/1A2 phenotyping is unlikely to substitute therapeutic drug monitoring of erlotinib in patients with advanced non-small cell lung cancer. PMID: 28444958
In Silico Predictions of Drug - Drug Interactions Caused by CYP1A2, 2C9 and 3A4 Inhibition - a Comparative Study of Virtual Screening Performance PMID: 27490388
Study corroborated results from a previous report that described interactions between ADORA2A and CYP1A2 polymorphisms and coffee consumption in Parkinson's disease. PMID: 28135712
CYP1A2 Polymorphisms are associated with lung cancer. PMID: 26987327
The study showed that the CYP1A2 (-163C>A) Rs762551 C/C genotype was associated with an increased risk of age-related macular degeneration. PMID: 28095090
findings suggest that CYP1A2 alleles or haplotypes were not predictive of metabolic phenotypes in the Nigerian population; carriers of CYP2A6*9/*17 genotype are likely to be poor metabolisers of CYP2A6 substrates and may experience adverse reactions or poor efficacy while using drugs metabolised mainly by CYP2A6 PMID: 28231062
frequency of slow activity CYP1A2 enzyme alleles is very low among Emiratis which correlates with the presence of low frequencies of derived alleles in CYP1A2 gene PMID: 28934216
Study provided additional evidence that CYP1A2 is an important susceptibility gene for essential hypertension. PMID: 27977510
the difference in clozapine levels between smokers and nonsmokers appeared to be significant in the individuals carrying the homozygous -163A allele (P=0.02). It was also found that nonsmokers carrying the -163A allele tended to have higher plasma levels of clozapine. Cigarette smoking has a significant impact on the plasma level of clozapine in schizophrenic patients carrying the homozygous -163A allele in the CYP1A2 gen PMID: 27203225
genetic association studies in population in Spain: Data suggest that an SNP in CYP1A2 (-163C>A; rs762551) is not associated with performance enhancement/visual attention effects in response to moderate caffeine consumption as dietary supplement (capsules) during exercise; C-allele carriers reported increased nervousness after caffeine ingestion. PMID: 28287486
We conducted a genome-wide association study (GWAS) of plasma caffeine, paraxanthine, theophylline, theobromine and paraxanthine/caffeine ratio. A single SNP at 6p23 (near CD83) and several SNPs at 7p21 (near AHR), 15q24 (near CYP1A2) and 19q13.2 (near CYP2A6) met GW-significance (P < 5 x 10-8) and were associated with one or more metabolites PMID: 27702941
We observed a strong dose-dependent increase in hair PhIP levels. This increase was highly correlated with dose received (rho = 0.68, P < 0.0001).CYP1A2 activity and normalizing for hair melanin did not modify the response to the intervention. PMID: 27207666
findings indicated that the -3860 G > A polymorphism in CYP1A2 might not be a risk factor for lung cancer [meta-analysis] PMID: 27323197
CYP1A2-163C/A polymorphism is associated with increased susceptibility to bladder cancer in the Chinese population. PMID: 27173252
rs762551 AA genotype may lead to higher coffee intake, especially in males, younger age groups, and individuals of Caucasian ethnicity. PMID: 27173183
Combined 3D-QSAR and docking procedures yielded precise information about the common and distinct interactions of inhibitors and the enzyme active sites of CYP1A2. PMID: 26648056
YP1A2 rs762551 was identified as a new potential predictive marker for early breast cancer events in AI-treated breast cancer patients. Moreover, combined genotypes of CYP1A2 rs762551 and CYP19A1 rs4646 or AhR Arg554Lys could further improve prediction of early AI-treatment response PMID: 27029552
The aim of the present study was to screen Uyghur volunteers for CYP1A2 genetic polymorphisms. PMID: 26383175
CYP1A2 -163A/A genotype influence carbamazepine pharmacokinetics. PMID: 26762380
niclosamide was subjected to efficient metabolic reactions hydroxylation and glucuronidation, wherein CYP1A2 and UGT1A1 were the main contributing enzymes, respectively. PMID: 26068521
Pregnane X receptor likely regulates CYP1A2 expression in 3D spheroids of liver cancer cells. PMID: 26643992
The results of this study suggest that the CYP1A2*1F polymorphism is associated with super-refractory schizophrenia. PMID: 26530626
This large meta-analysis suggests no significant effect of the investigated CYP1A2 SNPs on cancer overall risk under various genetic models PMID: 26865042
CYP1A2 rs762551 polymorphism is a protective factor for bladder cancer. PMID: 26514519
CYP rs2470890 allele 'C' showed an association with T2DM only when the interaction with coffee was considered, thereby setting an example of genetic activation by dietary changes associating with metabolic syndrome. PMID: 26588584
CYP1A2 metabolizes E2 to generate the potent anti-tumor agent 2-ME in HCC. PMID: 27093553
For the computed CYP 1A2 binding affinities, the model showed a root mean square error of 4.1 kJ mol-1 and a standard error in prediction in cross-validation of 4.3 kJ mol-1. PMID: 26551865
phenotypic association studies in population in Nigeria: Data suggest polymorphisms in CYP1A2 exist in the population studied; distribution of CYP1A2 activity is bimodal; influence of age/sex on activity are not significant in this biomarker study. PMID: 26098700
Dietary tocopherols inhibit PhIP-induced prostate carcinogenesis in CYP1A2-humanized transgenic mice. PMID: 26582657
The CYP1A2 -3860A genotype was associated with a decreased risk of breast cancer. In contrast, distributions of the -2467T/delT or -2467delT/delT and -163A/C or A/A genotypes among breast cancer patients and controls were similar. PMID: 25921178
Data suggest that short-term fasting up-regulates hepatic expression of CYP1A2 but down-regulates hepatic expression of CYP2C9 as compared with the post-absorptive state; only male subjects were studied. PMID: 25795462
Single-nucleotide polymorphisms alleles near BDNF that were associated with higher coffee consumption. PMID: 25288136
limb-truncal, but not orofacial tardive dyskinesia, is associated with CYP1A2 (-163C>, rs762551) polymorphism PMID: 25602162
Allele frequency of CYP1A2 polymorphisms in the Japanese population PMID: 25552922

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Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Protein Families

Cytochrome P450 family

Tissue Specificity

Liver.

Database Links

HGNC: 2596

OMIM: 108330

KEGG: hsa:1544

STRING: 9606.ENSP00000342007

UniGene: Hs.1361

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Code	CSB-RA178519A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, L02 whole cell lysate All lanes: CYP1A2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa IHC image of CSB-RA178519A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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CYP1A2 Recombinant Monoclonal Antibody

Product Details

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Target Background