Product Details

Uniprot No.

P17844

Target Names

DDX5

Alternative Names

Probable ATP-dependent RNA helicase DDX5 (EC 3.6.4.13) (DEAD box protein 5) (RNA helicase p68), DDX5, G17P1 HELR HLR1

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human DDX5

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2C3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

DDX5, also known as RNA helicase p68, is an ATP-dependent RNA helicase that plays essential roles in transcriptional regulation, RNA processing, and ribosome biogenesis. This DEAD box protein functions as a transcriptional co-activator and participates in alternative splicing, making it a significant target for researchers investigating epigenetics, nuclear signaling, and gene expression mechanisms.

This recombinant monoclonal antibody against DDX5 offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, clone 2C3 delivers lot-to-lot reproducibility that eliminates the variability often encountered with traditional hybridoma-derived antibodies. This sequence-defined approach ensures your results remain comparable across extended studies and between laboratory groups.

Extensive validation demonstrates this antibody's versatility across multiple applications. Western blot analysis confirms specific detection at the expected 70 kDa molecular weight across diverse sample types, including HepG2, HT-29, 293T, and Jurkat cell lysates, as well as mouse brain tissue, providing cross-species reactivity for both human and mouse research models. Immunohistochemistry validation in paraffin-embedded human colon cancer tissue shows clear nuclear staining patterns consistent with DDX5's known localization. Immunofluorescence studies in HepG2 cells further confirm nuclear detection, while flow cytometry analysis in HeLa cells demonstrates suitability for single-cell quantification approaches.

The broad application compatibility spanning Western blot, immunohistochemistry, immunofluorescence, flow cytometry, and ELISA gives researchers flexibility to characterize DDX5 expression and localization across experimental platforms. Whether investigating DDX5's role in transcriptional regulation, RNA metabolism, or its emerging connections to cancer biology, this antibody provides a reliable tool for advancing your research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.

Gene References into Functions

RIP-seq analysis in HEK293T cells identifies a complete repertoire of DDX5/p68 interacting transcripts including LOC284454 lncRNA. PMID: 29227193
Results showed that DDX5 was significantly up-regulated in gastric cancer tissues and revealed a novel role of DDX5 in gastric cancer cell proliferation via the mTOR pathway. PMID: 28216662
DDX5 is shown to be involved in RNA metabolism and viral infection, especially for RNA virus which seems to hijack host DDX5 to facilitate its own replication. However, DDX5 exhibits a role in antiviral responses during HBV and MYXV infection. Its opposite roles between DNA and RNA infection likely reflect the different modes of the biosynthesis of RNA and DNA viruses. [review] PMID: 29642538
A significant overlap between hnRNPA1 and DDX5 splicing targets and they share many closely linked binding sites. PMID: 30042133
The role of DDX5 in regulating esophageal cancer cell proliferation and tumorigenesis.DDX5 is highly expressed in esophageal cancer. PMID: 28244855
Downregulation of p68 RNA Helicase (DDX5) Activates a Survival Pathway Involving mTOR and MDM2 Signals.( PMID: 28557706
Results show refined biochemical and biological comparison of yeast Dbp2 and human DDX5 enzymes. Human DDX5 possesses a 10-fold higher unwinding activity than Dbp2, partially due to the presence of a mammalian/avian specific C-terminal extension. Also, ectopic expression of DDX5 rescues the cold sensitivity, cryptic initiation defects, and impaired glucose import in dbp2Delta cells, suggesting functional conservation. PMID: 28411202
p53 gain-of-function mutations accelerate endometrial carcinoma progression and metastasis by interfering with Drosha and p68 binding and pri-miR-26a-1 processing, resulting in reduced miR-26a expression and EZH2 overexpression. PMID: 26587974
Cervical cancer cell DDX5 gene is transcriptionally upregulated by calcitriol through a VDRE located in its proximal promoter. PMID: 26314252
Systematic Determination of Human Cyclin Dependent Kinase (CDK)-9 Interactome Identifies Novel Functions in RNA Splicing Mediated by the DEAD Box DDX5 and DDX17 RNA Helicases PMID: 26209609
LMTK3 escapes tumour suppressor miRNAs via sequestration of DDX5. PMID: 26739063
The data provide a model in which p68 and p53 interplay regulates PLK1 expression, and which describes the behavior of these molecules, and the outcome of their interaction, in human breast cancer. PMID: 24626184
Results show that a new mechanism of oncogenesis is attributed to p68 by upregulation of AKT and consequent nuclear exclusion and degradation of tumor suppressor FOXO3a. PMID: 25745998
DDX5 played an important role in the proliferation and tumorigenesis of non-small-cell lung cancer cells by activating the beta-catenin signaling pathway. PMID: 26212035
study shows that correction of p68 may reduce toxicity of the mutant RNAs in DM1 and in DM2 PMID: 26080402
Data indicate that cyclooxygenase 2 (COX-2) correlates inversely to microRNA 183 (miR-183) and directly to DEAD-box helicase p68 (DDX5). PMID: 25963660
DDX5 protein is essential for normal cell proliferation; (2) the transition from G1 to S/G2 phase is accompanied by an increase of DDX5 protein concentration in the cells. PMID: 26035968
AML is dependent on DDX5 and that inhibiting DDX5 expression slows AML cell proliferation. PMID: 24910429
Downregulation of DDX5 and DDX17 protein expression during myogenesis and epithelial-to-mesenchymal transdifferentiation contributes to the switching of splicing programs during these processes. PMID: 24910439
Data indicate that armadillo repeat protein ARVCF interacts with the splicing factors the splicing factor SRSF1 (SF2/ASF), the RNA helicase p68 (DDX5), and the heterogeneous nuclear ribonucleoprotein hnRNP H2. PMID: 24644279
In conclusion, authors identified DDX5 as a positive regulator for Japanese encephalitis virus replication via its binding to viral 3' UTR. PMID: 24035833
DDX5 facilitates HIV-1 replication as a cellular co-factor of Rev. PMID: 23741449
DDX5 might be critical for NOTCH1-mediated T-ALL pathogenesis and thus is a potential new target for modulating the Notch signaling in leukemia PMID: 23108395
p68, in the presence of Ca-calmodulin, can function as a microtubule motor. PMID: 23322042
Results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment. PMID: 22750847
High p68 RNA helicase expression is associated with glioma. PMID: 22810421
Data indicate that transcriptional coregulator ddx5/ddx17 RNA helicases can simultaneously regulate the transcriptional activity and alternative splicing of NFAT5 transcription factor. PMID: 22266867
RNA helicases Ddx17 and Ddx5 contribute to tumor-cell invasiveness by regulating alternative splicing of several DNA- and chromatin-binding factors, including the macroH2A1 histone. PMID: 23022728
The DEAD box RNA helicase p68, also referred to as DDX5, directly interacts with VDR. PMID: 22476084
there is a direct interaction between DDX5 and NS5B and DDX5 has two independent NS5B-binding sites PMID: 22640416
High DDX5 is associated with basal breast cancer cells. PMID: 22086602
Using an RNA affinity pulldown-coupled mass spectrometry approach the study identified DDX5/RNA helicase p68 as an activator of TAU exon 10 splicing. PMID: 21343338
a striking inverse association of p68 and delta133p53 expression in primary breast cancers was identified. PMID: 20818423
DEAD-box RNA helicase p68 (DDX5) and its associated noncoding RNA, steroid receptor RNA activator (SRA), form a complex with CTCF that is essential for insulator function PMID: 20966046
Pleiotropic effects of p300-mediated acetylation on p68 and p72 RNA helicase. PMID: 20663877
crystallization and preliminary diffraction analysis of N-terminal region of DDX5 is reported.X-ray diffraction data were processed to a resolution of 2.7 A. PMID: 20124720
DDX5 is a repressor of fibrogenic genes in HSCs through interaction with transcriptional complexes. PMID: 20022962
Essential for pre-mrna splicing in vitro; may function in destabilizing the U1-5'ss interaction. Depletion of p68 RNA helicase arrested spliceosome assembly at the prespliceosome stage PMID: 12101238
synergism with transcriptional coactivators CBP and p300 PMID: 12527917
role in c-H-ras alternative splicing regulation PMID: 12665590
p68 is an important transcriptional regulator, functioning as a co-activator and/or co-repressor depending on the context of the promoter & the transcriptional complex. AA 1-478 of p68 can repress transcription as well as the full-length protein. PMID: 15298701
there is a tightly controlled expression and nucleolar localization of p68 in keratinocytes in vitro and during skin repair in vivo that functionally contributes to keratinocyte proliferation and gene expression PMID: 15304501
mechanism by which p68 may act as a tumour cosuppressor in governing p53 transcriptional activity PMID: 15660129
data suggest that function(s) of p68 RNA helicase may be subjected to the regulation of multiple cell signal pathways PMID: 15927448
In addition, it could be demonstrated that increasing the Tlk1 activity in HT1080 cells by forced Tlk1 overexpression leads to an increased phosphorylation of endogenous p68. PMID: 15950181
Patient with chronic hepatitis C carrying DDX5 haplotypes are at an increased risk of developing advanced liver fibrosis. PMID: 16697732
SUMO modification of the DEAD box protein p68 modulates its transcriptional activity and promotes its interaction with HDAC1 PMID: 17369852
A mutant that carries mutations at the phosphorylation sites (Y593/595F) dramatically sensitizes TRAIL-resistant cells to TRAIL-induced apoptosis, suggesting a potential therapeutic strategy to overcome TRAIL resistance. PMID: 17384675
The percentage correlation between Q-RT-PCR and microarray were 70% and 48% by using DDX5 and GAPDH as internal controls, respectively. PMID: 17540040
p68/p72 may contribute to colon cancer formation by directly up-regulating proto-oncogenes and indirectly by down-regulating the growth suppressor p21(WAF1/CIP1). PMID: 17699760

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Subcellular Location

Nucleus. Nucleus, nucleolus. Cytoplasm.

Protein Families

DEAD box helicase family, DDX5/DBP2 subfamily

Database Links

HGNC: 2746

OMIM: 180630

KEGG: hsa:1655

STRING: 9606.ENSP00000225792

UniGene: Hs.279806

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Code	CSB-RA231179A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HepG2 whole cell lysate, HT-29 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue All lanes: DDX5 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 70, 61 kDa Observed band size: 70 kDa IHC image of CSB-RA231179A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 Cells with CSB-RA231179A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Overlay histogram showing Hela cells stained with CSB-RA231179A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/110⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/110⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.
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DDX5 Recombinant Monoclonal Antibody

Product Details

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Target Background