Product Details

Uniprot No.

Target Names

EIF4A3

Alternative Names

ATP-dependent RNA helicase DDX48 antibody; ATP-dependent RNA helicase eIF4A-3 antibody; DDX48 antibody; DEAD box protein 48 antibody; eIF-4A-III antibody; eIF4A-III antibody; EIF4A3 antibody; eIF4AIII antibody; Eukaryotic initiation factor 4A-III antibody; Eukaryotic initiation factor 4A-like NUK-34 antibody; Eukaryotic translation initiation factor 4A isoform 3 antibody; hNMP 265 antibody; IF4A3_HUMAN antibody; NMP 265 antibody; NMP265 antibody; Nuclear matrix protein 265 antibody; NUK34 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human EIF4A3

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6E9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

EIF4A3 serves as a critical ATP-dependent RNA helicase and core component of the exon junction complex, playing essential roles in nonsense-mediated mRNA decay, mRNA splicing, and translation regulation. This DEAD-box protein has emerged as a significant focus in cancer research, where its dysregulation has been linked to tumor progression and therapeutic resistance, making it a valuable target for investigators studying RNA metabolism and disease mechanisms.

This recombinant monoclonal antibody, clone 6E9, offers researchers the reproducibility advantages inherent to recombinant technology. Generated against a synthetic peptide derived from human EIF4A3 and produced in rabbit, the antibody provides sequence-defined consistency between lots, eliminating the variability concerns that can complicate long-term studies or multi-site collaborations. Affinity chromatography purification ensures high specificity for your target.

Validation studies demonstrate reliable performance across multiple experimental platforms. Immunofluorescence analysis in HeLa cells reveals clear nuclear localization patterns consistent with EIF4A3's known role in mRNA processing, with recommended dilutions of 1:50-1:200 providing flexibility to optimize signal intensity for your specific imaging setup. Flow cytometry validation using Jurkat cells confirms the antibody's utility for quantitative single-cell analysis, showing distinct positive population shifts compared to isotype controls at 1:50-1:200 dilutions. The antibody is also validated for ELISA applications.

For researchers investigating RNA surveillance pathways, splicing regulation, or the emerging connections between EIF4A3 and oncogenic processes, this antibody provides a dependable tool for characterizing protein expression and localization in human cell models. Its compatibility with both imaging and flow-based approaches supports diverse experimental strategies within a single research program.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. Involved in craniofacial development.

Gene References into Functions

FAL1 may work as a ceRNA to modulate AKT1 expression via competitively binding to miR-637 in HSCR. PMID: 30062828
we firstly report that the lncRNA H19 recruits eIF4A3 and promotes the colorectal cancer cell proliferation PMID: 26989025
High EIF4A expression is associated with malignant peripheral nerve sheath tumors and vestibular schwannomas. PMID: 26951381
MYC, DUX4, and EIF4A3 might contribute to facioscapulohumeral dystrophy pathophysiology PMID: 28273136
Host eIF4AIII RNA helicase is required for efficient human cytomegalovirus replication. PMID: 26773380
EIF4A3 is a gene involved in RNA metabolism, plays a role in mandible, laryngeal, and limb morphogenesis. Richieri-Costa-Pereira syndrome is caused by mutations in EIF4A3. PMID: 24360810
The binding mode of CWC22 to eIF4AIII reveals a facet of how MIF4G domains use their versatile structural frameworks to activate or inhibit DEAD-box proteins. PMID: 24218557
CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly PMID: 22961380
demonstrate direct physical interactions between yeast Sgd1p and Fal1p, and between their human orthologs (NOM1 and eIF4AIII) in vitro and in vivo, identifying human NOM1 as a missing eIF4G-like interacting partner of eIF4AIII PMID: 21576267
identification of eIF4AIII and Barentsz as components of a conserved protein complex that is essential for mRNA localization in flies and nonsense-mediated mRNA decay in mammals PMID: 14973490
eIF4AIII represents a new functional class of DExH/D box proteins that act as RNA clamps or 'place holders' for the sequence-independent attachment of additional factors to RNAs. PMID: 15034551
detection of autoantibodies to DDX48 may have clinical utility for the improved diagnosis of pancreatic cancer PMID: 15796914
The stable association of multiprotein exon junction complex core with RNA is maintained by inhibition of eIF4AIII ATPase activity by MAGOH-Y14. PMID: 16170325
crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase eukaryotic initiation factor 4AIII bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic PMID: 16931718
Although it is dispensable for pre-mRNA splicing in vitro, eIF4A3 is required for splicing-dependent loading of the Y14-Magoh core heterodimer onto mRNA. PMID: 17606899
Data show that eIF4AIII, a core exon junction complex (EJC) component loaded onto mRNAs by pre-mRNA splicing, is associated with neuronal mRNA granules and dendritic mRNAs. PMID: 17632064
The nonsense-mediated-decay mRNA surveillance pathway downregulates aberrant E-cadherin transcripts in gastric cancer cells and in CDH1 mutation carriers. PMID: 18427545
NMP 265, a common part of the nuclear matrix, is located in the nucleus via an N-terminal amino acid sequence. PMID: 10623621

Hide All

Involvement in disease

Richieri-Costa-Pereira syndrome (RCPS)

Subcellular Location

Nucleus. Nucleus speckle. Cytoplasm.

Protein Families

DEAD box helicase family, eIF4A subfamily

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 18683

OMIM: 268305

KEGG: hsa:9775

STRING: 9606.ENSP00000269349

UniGene: Hs.389649

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Code	CSB-RA965999A0HU
Size	US$210
	Order now
Image	Immunofluorescence staining of Hela cell with CSB-RA965999A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA965999A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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EIF4A3 Recombinant Monoclonal Antibody

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Target Background