Product Details

Uniprot No.

Target Names

EWSR1

Alternative Names

bK984G1.4 antibody; bK984G1.4 Ewing sarcoma breakpoint region 1 protein antibody; Ewing sarcoma breakpoint region 1 antibody; Ewing sarcoma breakpoint region 1 protein antibody; Ewings sarcoma EWS Fli1 type 1 oncogene antibody; EWS antibody; EWS oncogene antibody; EWS RNA binding protein 1 antibody; EWS_HUMAN antibody; EWSR 1 antibody; Ewsr1 antibody; EWSR1 protein antibody; RNA binding protein EWS antibody; RNA-binding protein EWS antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human EWSR1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3F1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

EWSR1 (Ewing Sarcoma Breakpoint Region 1) is an RNA-binding protein that plays a central role in transcriptional regulation and RNA processing. This multifunctional protein gained significant research attention due to its involvement in chromosomal translocations that generate oncogenic fusion proteins, most notably the EWS-FLI1 fusion characteristic of Ewing sarcoma. Beyond its role in cancer biology, EWSR1 participates in diverse cellular processes including gene expression regulation, DNA damage response, and cellular stress responses, making it a valuable target for researchers investigating both normal cellular physiology and disease mechanisms.

This recombinant monoclonal antibody, clone 3F1, offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit host and monoclonal nature ensure high specificity for human EWSR1.

Validation across multiple applications demonstrates genuine experimental flexibility. Western blot analysis successfully detects EWSR1 across a diverse panel of human cell lines including MCF7, K562, Jurkat, A431, THP-1, HEK293, and U251MG, with an observed band at approximately 85 kDa. This migration above the predicted 68 kDa molecular weight likely reflects post-translational modifications such as glycosylation, which are well-documented for this protein. Immunohistochemistry validation in human lung cancer tissue confirms utility for tissue-based studies, while immunofluorescence in HeLa cells reveals nuclear localization patterns. Flow cytometry applications have also been validated, expanding options for single-cell analysis.

This antibody serves researchers investigating Ewing sarcoma pathogenesis, RNA biology, and transcriptional regulation mechanisms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Gene References into Functions

ATG4B expression was observed markedly upregulated by EWS-FLI1 overexpression, and silencing of ATG4B dramatically inhibits autophagy in Ewing sarcoma cells. PMID: 28902354
Studies demonstrate that the translocation-derived fusion protein EF (EWS-FLI1) misregulates numerous genes involved in metabolism suggesting that EF is a master regulator of metabolic reprogramming in Ewing sarcoma, diverting metabolites toward biosynthesis. PMID: 28720588
Together, the data demonstrate the critical requirement of GGAA-microsatellites as EWS/FLI activating response elements in vivo and reveal an unexpected role for the EWS portion of the EWS/FLI fusion in binding to sweet-spot GGAA-microsatellites. PMID: 28847958
EWSR1 fusion protein is required for PAX7 expression in Ewing sarcoma and identify a candidate EWSR1-FLI1-bound PAX7 enhancer that coincides with both a consensus GGAA repeat-containing binding site and a peak of regulatory H3K27 acetylation. PMID: 28643791
In the 3 cases of the primary extraskeletal myxoid chondrosarcoma (EMC) of bone we found the most frequent and specific chromosomal translocation t(9:22) EWSR1-NR4A3 of the extraskeletal counterpart. PMID: 28249774
a breakpoint in the EWSR1-ATF1 fusion to be the same as that reported in HCCC. Established CCOC-T cells grew extremely slowly, but the cells showed highly invasive activity PMID: 28559020
study proposed that EWSR1 rearrangement was present in a subset of myoepithelial tumors of salivary glands, with variable morphological features PMID: 28648935
EWSR1 Gene Rearrangement is associated with Endometrial Primitive Neuroectodermal Tumor. PMID: 28801375
-cell lymphoblastic leukemia with t(11;22)(q24;q12) and EWSR1 rearrangement. PMID: 28104675
Kinetics of EWSR1 fusion sequence copy numbers in the plasma are correlated with changes of the tumor volume in patients with localized and metastatic disease. PMID: 27283964
Authors found that FUS, EWS and TAF15 expression is differentially regulated during brain development, both in time and in space. In particular, this study identifies a fine-tuned regulation of FUS and EWS during neuronal differentiation. PMID: 28453628
Recent advances in biologic and genomic understanding of these two cancers has expanded the potential for therapeutic advancement and prevention. In Ewing sarcoma, directed focus on inhibition of EWSR1-FLI1 and its effectors has produced promising results. PMID: 28561686
data suggest EWS/FLI binds to "promoter-like" and "enhancer-like" microsatellites to mediate activation and repression of target genes through different regulatory mechanisms PMID: 29091716
As spontaneous fluctuations in EWS-FLI1 levels of Ewing sarcoma cells in vitro and in vivo, associated with a switch between a proliferative, non-migratory EWS-FLI1-high and a non-proliferative highly migratory EWS-FLI1-low state, were recently described, our data provide a mechanistic basis for the underlying EWS-FLI1-dependent reversible cytoskeletal reprogramming of Ewing sarcoma cells. PMID: 28671673
Fusion of short fragments of EWSR1 to FLI1 is sufficient to recapitulate BAF complex retargeting and EWS-FLI1 activities; studies thus demonstrate that the physical properties of prion-like domains can retarget critical chromatin regulatory complexes to establish and maintain oncogenic gene expression programs. PMID: 28844694
Case Reports: maxillary sinus clear cell carcinomas with EWSR1-ATF1 gene fusion. PMID: 27916624
Results expand the spectrum of tumor types harboring EWSR1/FUS-ATF1 gene fusions to include a subgroup of conventional epithelioid malignant mesothelioma. PMID: 28505004
papillary thyroid carcinomas with EWSR1 rearrangement disclosed a lower percentage of nuclei with EWSR1 polysomy than those without EWSR1 rearrangement PMID: 28236059
The net result of combined lurbinectedin and irinotecan treatment was a complete reversal of EWS-FLI1 activity and elimination of established tumors in 30% to 70% of mice after only 11 days of therapy. Our results illustrate the preclinical safety and efficacy of a disease-specific therapy targeting the central oncogenic driver in Ewing sarcoma. PMID: 27697767
Aggregation of FET proteins FUS, EWSR1, and TAF15 mediate a pathological change in amyotrophic lateral sclerosis. (Review) PMID: 27311318
A subgroup of MAML2 fusion-negative mucoepidermoid carcinomas are actually clear cell carcinoma of the salivary gland with EWSR1 translocation. PMID: 27769871
Study found consistent DNA hypomethylation at enhancers regulated by the disease-defining EWS-FLI1 fusion protein, thus establishing epigenomic enhancer reprogramming as a ubiquitous and characteristic feature of Ewing sarcoma. PMID: 28134926
Report a distinct group of myxoid mesenchymal neoplasms occurring in children or young adults with a predilection for intracranial locations with EWSR1-AFT1/CREB1/CREM fusions. PMID: 28009602
We conclude that in the context of 22q11-12 regional alterations present in SMARCB1-deleted tumors, simultaneous EWSR1 involvement may be misinterpreted as equivalent to EWSR1 rearrangement. A detailed clinicopathologic correlation and supplementing the EWSR1 FISH assay with complementary methodology is mandatory for correct diagnosis PMID: 27218413
While our study confirmed that fluorescence in-situ hybridization is a sensitive and specific tool in the diagnosis of EWSR1-associated tumours, atypical fluorescence in-situ hybridization signals and classical rearrangement in entities other than EWSR1-associated tumours can occur PMID: 27385661
EWSR1 regulates the acetylation of microtubules in a cell cycle-dependent manner through its dynamic location on spindle MTs, and may be a novel regulator for mitosis progress independent of its translocation. PMID: 27341063
Studies provide evidence that FUS/TLS, EWS and TAF15 proteins play a major role in neurodegenerative disorders. (review). PMID: 27415968
We identified EWSR1 rearrangement in 25% of Ectomesenchymal chondromyxoid tumour PMID: 27010880
EWSR1-related rearrangement was detected extraskeletal myxoid chondrosarcoma PMID: 27402218
a novel EWSR1/ETS chimeric gene, was identified in a patient diagnosed with refractory AML, suggesting a potential role of leukemogenesis in rare cases of AML. This fusion gene is very likely to exhibit oncogenic potential by interfering with the p53/p21-dependent pathway. PMID: 27627705
LRWD1 is a novel regulator of EWS-FLI1 driven cell proliferation in Ewing sarcoma cells. EWS-FLI1 regulates LRWD1 expression and LRWD1 may contribute to EWS-FLI1 mediated transcriptional repression. PMID: 27760381
Ewing sarcoma may be susceptible to treatment with epigenetic inhibitors blocking BRD3/4 activity and the associated pathognomonic EWS-FLT1 transcriptional program. PMID: 26623725
FUS and EWS target genes involved in pathways at the RNA regulatory level PMID: 26573619
Identify SP1 and PI3K/AKT signaling as modulators of EWS/FLI1 gene expression in tumor cell lines. PMID: 26336820
Case Reports: 2 girls with primary renal myoepithelial carcinomas with a novel EWSR1-KLF15 fusion. PMID: 26523541
3'-UTR poly(T/U) repeat of EWSR1 is altered in microsatellite unstable colorectal cancer. PMID: 25930744
study investigated EWSR1 status in ovarian hemangiomas; all cases were negative for EWSR1 rearrangement; 2 cases demonstrated additional intact copies of EWSR1 indicating aneusomy 22 or structural abnormality of chromosome 22 resulting in apparent duplication of the EWSR1 gene region PMID: 25851709
The paper investigates the presence of EWS/FLI-1 fusion in clinically diagnosed sarcoma belonging to different non-Ewing connective tissue tumors. PMID: 25914746
Results show that RNA-binding protein EWS binds and regulates CCDC6 expression at RNA and protein levels. PMID: 25751255
KLF17 is also a rare gene fusion partner to EWSR1-rearranged myoepithelial tumors. PMID: 25706482
The impairment of EWS-dependent midzone formation via the recruitment of Aurora B is a potential mechanism of Ewing sarcoma development. PMID: 25483190
Results show that although the cosegregation of the EWSR1 p.R471C substitution in the index family could not be established in people suffering from essential tremor, the EWSR1 p.R471C substitution is a candidate variant that needs to be further screened. PMID: 25375143
A key function of EWS-FLI1 in tumorigenesis is to maintain the epigenetic memory of activated homeobox genes programs. PMID: 25625846
Data suggest that aberrant cell cycle activation in Ewing sarcoma is due to the de-repression of transcription factor E2F targets of transcriptional induction and physical recruitment of E2F3 by fusion protein EWS-FLI1 replacing E2F4 on their promoters. PMID: 25712098
EWS/FLI-induced repression of alpha5 integrin and zyxin expression promotes tumor progression by supporting anchorage-independent cell growth. PMID: 25057021
These experiments establish systemic alternative splicing as an oncogenic process modulated by EWS-FLI1. PMID: 25737553
Case Reports: genetically confirmed primary renal sclerosing epithelioid fibrosarcoma with EWSR1-CREB3L1 gene fusion. PMID: 25353281
Describe EWSR1 gene rearrangement in a subset of myoepithelial carcinomas arising in minor and major salivary glands. PMID: 25581728
EWSR1-ATF1 fusion gene was found in hyalinizing clear cell carcinoma PMID: 25359601
EWSR1 rearrangement is associated with ewing sarcomas. PMID: 24293381

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Involvement in disease

Ewing sarcoma (ES); Angiomatoid fibrous histiocytoma (AFH)

Subcellular Location

Nucleus. Cytoplasm. Cell membrane. Note=Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.

Protein Families

RRM TET family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 3508

OMIM: 133450

KEGG: hsa:2130

STRING: 9606.ENSP00000400142

UniGene: Hs.374477

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Code	CSB-RA567639A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: MCF7 whole cell lysate(30µg), K562 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), A431 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), U251MG whole cell lysate(30µg) All lanes:EWSR1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 68 kDa Observed band size: 85 kDa Exposure time：2min IHC image of CSB-RA567639A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HeLa cell with CSB-RA567639A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA567639A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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EWSR1 Recombinant Monoclonal Antibody

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