Code | CSB-RA599469A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
F10 antibody-generating hybridomas were created by the fusion of myeloma cells with B cells isolated from the animal that was immunized with a synthetic peptide derived from human factor X. The variable light and variable heavy domains of hybridomas were sequenced to construct a vector for a recombinant generation. Subsequently, the vector containing F10 monoclonal antibody gene was transfected into cells for cultivation, and the recombinant monoclonal antibody was isolated and purified using affinity chromatography from the cell culture supernatant. The purified antibody was tested for specific human F10 protein detection in ELISA and IHC applications.
Coagulation factor X (F10), also known as FX or thrombokinase, is a vitamin K-dependent serine protease that plays a crucial role in the coagulation cascade. Its main function is to activate prothrombin to thrombin, which then converts fibrinogen to fibrin, leading to the formation of a blood clot. Factor X is activated by either the intrinsic pathway or the extrinsic pathway of coagulation, both of which ultimately lead to the activation of factor X by either factor VIIIa and factor IXa or factor VIIa and tissue factor, respectively. Factor X also plays a role in other biological processes, such as inflammation, cell migration, and tissue repair.
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