FAM50A Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA095663A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: A549 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), Jurkat whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: FAM50A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time：1min IHC image of CSB-RA095663A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HeLa cell with CSB-RA095663A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q14320

Target Names

FAM50A

Alternative Names

9F antibody; DXS9928E antibody; FA50A_HUMAN antibody; FAM 50A antibody; fam50a antibody; Family with sequence similarity 50 member A antibody; HXC 26 antibody; HXC26 antibody; OTTHUMP00000061460 antibody; Protein FAM50A antibody; Protein HXC 26 antibody; Protein HXC-26 antibody; Protein HXC26 antibody; Protein XAP 5 antibody; Protein XAP-5 antibody; Protein XAP5 antibody; XAP 5 antibody; XAP 5 protein antibody; XAP5 antibody; XAP5 gene antibody; XAP5 protein antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human FAM50A protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Probably involved in the regulation of pre-mRNA splicing.

Subcellular Location

Nucleus.

Protein Families

FAM50 family

Tissue Specificity

Widely expressed in fetal and adult tissues. Mostly abundant in fetal brain, liver and kidney; in the adult, high levels were also observed in heart, skeletal muscle, spleen, thymus, prostate and small intestine. Expressed in fetal cerebellum and hypothal

Database Links

HGNC: 18786

OMIM: 300453

KEGG: hsa:9130

STRING: 9606.ENSP00000377225

UniGene: Hs.54277

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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