FDX1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA577140A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg), A549 whole cell lysate(30µg), U-87MG whole cell lysate(30µg), Mouse kidney tissue lysate(30µg) All lanes: Ferredoxin 1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 19 kDa Observed band size: 13 kDa Exposure time：2min IHC image of CSB-RA577140A0HU diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA577140A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-RA577140A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA577140A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

P10109

Target Names

FDX1

Alternative Names

Adrenal ferredoxin; Adrenodoxin; Adrenodoxin; mitochondrial; ADX; ADX_HUMAN; FDX; fdx1; Ferredoxin 1; Ferredoxin-1; Hepatoredoxin; LOH11CR1D; Mitochondrial adrenodoxin; mitochondrial

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human FDX1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8D3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Ferredoxin-1 (FDX1) is a mitochondrial iron-sulfur protein essential for electron transfer in steroid hormone biosynthesis and vitamin D metabolism. As a critical component of the cytochrome P450 system in steroidogenic tissues, FDX1 facilitates the conversion of cholesterol to pregnenolone and participates in cortisol synthesis. Recent research has also identified FDX1 as a key mediator of cuproptosis, a copper-dependent form of regulated cell death, positioning this protein at the intersection of metabolic regulation and emerging cell death pathways.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human FDX1, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced from a single rabbit clone (8D3), it eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations.

Validation across multiple platforms demonstrates versatile performance for diverse experimental approaches. Western blot analysis detects FDX1 in human cell lines including HEK293, A549, and U-87MG, as well as mouse kidney tissue, confirming cross-species reactivity. The observed band at 13 kDa differs from the predicted 19 kDa molecular weight, likely reflecting post-translational processing of the mitochondrial targeting sequence upon import. Immunohistochemistry staining in human adrenal gland tissue confirms detection in a physiologically relevant steroidogenic context, while colorectal cancer tissue sections extend utility to oncology research. Immunofluorescence in HepG2 cells and flow cytometry analysis in HeLa cells further demonstrate compatibility with single-cell applications.

This antibody serves researchers investigating steroidogenesis, mitochondrial metabolism, copper-induced cell death mechanisms, and the emerging role of FDX1 in cancer biology.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Essential for the synthesis of various steroid hormones. Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage. Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons.

Gene References into Functions

Active Site Structures of CYP11A1 in the Presence of Its Physiological Substrates and Alterations upon Binding of Adrenodoxin PMID: 28991453
Data suggest that binding sites between CYP11B1/CYP11B2 and adrenodoxin/ferredoxin-1 exhibit electrostatic interactions at K370 in CYP11B1 and at K366 in CYP11B2 mutant R366K with D79 in adrenodoxin/ferredoxin-1. (CYP11B1 = cytochrome P450 family 11 subfamily B member 1; CYP11B2 = cytochrome P450 family 11 subfamily B member 2) PMID: 28355486
FDX1 and FDX2 both bind NFS1 and donate electrons for iron-sulfur cluster biosynthesis. PMID: 28001042
TNFSF13 and FDX1 have potential roles in IgAN in the Han Chinese population. This information may be useful in the development of early prognostics for IgAN. PMID: 26431901
Did not find any positive association between FDX1 SNPs and the risk of IgA nephropathy after adjustment for age and sex, but did find a significant and strong correlation with relevant clinical pathological parameters. PMID: 26370181
results indicate transcription of FDX1 is regulated by the NR5A family and cAMP signaling, and participates in steroid hormone production in ovarian granulosa ce PMID: 23435367
Data suggest that interference with any of the three related genes, ferredoxin (FDX)1, FDX2 or FDXR, disrupts iron-sulfur cluster assembly and maintenance of normal cytosolic and mitochondrial iron homeostasis. PMID: 22101253
Results present the crystal structure of the complex of human adrenodoxin and CYP11A1--the first of a complex between a eukaryotic CYP and its redox partner. PMID: 21636783
Unlike Fdx1, Fdx2 was unable to efficiently reduce mitochondrial cytochromes P450 and convert steroids, indicating that the two ferredoxin isoforms are highly specific for their substrates in distinct biochemical pathways. PMID: 20547883
ADX rate of hydroxylation was linear with incubation time. PMID: 12782149
Adrenodoxin induces apoptosis by the generation of reactive oxygen species in mitochondria PMID: 15927889
Adx is able to support reactions catalyzed by human microsomal P450s: full length CYP17, truncated CYP17, and truncated CYP21 PMID: 17188650

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Subcellular Location

Mitochondrion matrix.

Protein Families

Adrenodoxin/putidaredoxin family

Tissue Specificity

Highest levels in the adrenal gland (at protein level). Also detected in kidney and testis (at protein level).

Database Links

HGNC: 3638

OMIM: 103260

KEGG: hsa:2230

STRING: 9606.ENSP00000260270

UniGene: Hs.744

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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