Product Details

Uniprot No.

Target Names

Alternative Names

Peptidyl-prolyl cis-trans isomerase FKBP4 (PPIase FKBP4) (EC 5.2.1.8) (51 kDa FK506-binding protein) (FKBP51) (52 kDa FK506-binding protein) (52 kDa FKBP) (FKBP-52) (59 kDa immunophilin) (p59) (FK506-binding protein 4) (FKBP-4) (FKBP59) (HSP-binding immunophilin) (HBI) (Immunophilin FKBP52) (Rotamase) [Cleaved into: Peptidyl-prolyl cis-trans isomerase FKBP4, N-terminally processed], FKBP4, FKBP52

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human FKBP4 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1G5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

FKBP4, also known as FKBP52, is a 52 kDa immunophilin that functions as a peptidyl-prolyl cis-trans isomerase and plays essential roles in steroid receptor signaling and protein folding pathways. As a key component of mature steroid receptor complexes, FKBP4 influences glucocorticoid, progesterone, and androgen receptor activity, making it a significant target in studies of hormone-dependent processes, stress response mechanisms, and related disease states.

This recombinant monoclonal antibody, generated in rabbit using a synthesized peptide from human FKBP4, offers the consistency and reproducibility that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant expression, researchers can expect reliable performance across experiments and between lots, eliminating a common source of variability in long-term studies.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis detects FKBP4 in both human HEK293T and mouse NIH/3T3 cell lysates, confirming cross-species reactivity and revealing a band at approximately 55 kDa. The slight shift from the predicted 52 kDa molecular weight likely reflects post-translational modifications such as phosphorylation, which is consistent with FKBP4's known regulatory modifications. Immunohistochemistry performed on human thyroid tissue sections shows clear target localization, while immunofluorescence and flow cytometry applications have been validated using MCF-7 cells, providing options for both fixed-cell imaging and quantitative single-cell analysis.

Whether investigating steroid hormone signaling, chaperone biology, or protein folding mechanisms, this antibody provides a dependable tool for detecting FKBP4 across tissue sections, cultured cells, and lysate-based assays.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.

Gene References into Functions

ID4 selectively regulates AR activity through direct interaction with FKBP52. PMID: 28252832
FKBP52 could be abnormally released from NFTs negative neurons in AD brains in correlation with the early pathologic Tau-D(421) neuronal accumulation. PMID: 27479154
Mechanistically, USP49 deubiquitinates and stabilizes FKBP51, which in turn enhances PHLPP's capability to dephosphorylate AKT. PMID: 28363942
Low expression of FKBP4 is associated with Progesterone Resistance in Endometriosis. PMID: 27778641
Results provide a molecular mechanism by which FKBP52 modulates telomerase activity by promoting dynein-dynactin-dependent nuclear import of hTERT. PMID: 27503910
The Hsp90-associated FKBP52 cochaperone has become increasingly associated with aberrant steroid hormone receptor signaling in disease. [review] PMID: 25986565
The capacity FKBP52 to oligomerize Tau is not linked to its peptidyl-prolyl isomerase activity. PMID: 26903089
FKBP52 and beta-catenin interact directly in vitro. FKBP52 promotes beta-catenin interaction with androgen receptor signaling. PMID: 26207810
FKBP4 was not differentially expressed in PTSD patients with low HPA axis reactivity compared to PTSD patients with high HPA axis reactivity. PMID: 25745955
FKBP51 is the major target accounting for the neuritotrophic effect of neuroimmunophilin ligands. Selectivity against the homolog FKBP52 is essential for optimal neuritotrophic efficacy. PMID: 25615537
identify a novel steroid-responsive FKBP52-dependent pathway suppressing the expression and activity of tryptophan-2,3-dioxygenase PMID: 25132599
FKBP52 seems to be disrupted in preeclampsia and intrauterine growth restriction pregnancies PMID: 26065228
The biological action of NF-kappaB in different cell types could be positively regulated by a high FKBP52/FKBP51 expression ratio. PMID: 25104352
Molecular chaperone activity and biological regulatory actions of the TPR-domain immunophilins FKBP51 and FKBP52 PMID: 24694367
Despite their substantial structural similarity, in both the beta3 bulge and the beta4-beta5 loop, the FK1 domain of FKBP51 undergoes significantly populated conformational transitions that appear to be suppressed in FKBP52. PMID: 24749623
FKBP52 appears to be an endogenous candidate that directly interacts with the pathogenic Tau-P301L and modulates its function in vitro and in vivo PMID: 24623856
The guinea pig GR-specific mutations within the H1-H3 loop confer global changes within the GR-Hsp90 complex that favor FKBP51 repression over FKBP52 potentiation. PMID: 23686112
FKBP4, p23, and Aha1 cooperatively regulate the progression of hAgo2 through the chaperone cycle. PMID: 23741051
This study does not confirm a role for genetic variants in the SFRS3 and FKBP4 genes in the pathogenesis of corticosteroid-induced ocular hypertension. PMID: 22921020
involved in the induction of decidualization PMID: 22279148
FKBP52 expression level is abnormally low in frontal cortex of Alzheimer's disease compared to controls. PMID: 22233767
Aimed to discover markers of drug resistance in breast cancer before neoadjuvant chemotherapy. Found FKBP4 and S100A9 might be putative prediction markers in discriminating the drug resistant group from the drug sensitive group of breast cancer patients. PMID: 22074005
Transgenic overexpression of HSP56 does not result in cardiac hypertrophy nor protect from ischaemia/reperfusion injury. PMID: 20932935
these results provide evidence that FKBP5 transcriptional dysregulation together with FKBP4 as its functional antagonist are implicated in biological features of major depressive disorder symptoms in human immunodeficiency virus-infected individuals. PMID: 20726698
RET51/FKBP52 complex is involved in Parkinson disease. PMID: 20442138
Data show that FKBP52, which is abundant in brain, binds directly and specifically to Tau, especially in its hyperphosphorylated form. PMID: 20133804
activation of the Wnt pathway and mutation of the tcf-4 gene in hepatocellular carcinoma (HCC) PMID: 12603528
Data show that FK506-binding protein 52(FKBP52) selectively potentiates hormone-dependent reporter gene activation, and this potentiation is readily blocked by co-expression of the closely related FKBP51. PMID: 12606580
Promoter constructs with only 143 bp of upstream sequence contain a CAAT motif sequence and consensus binding sites for Sp1, heat-shock factor, and MYC-MAX. The sequence maintained high activity when transfected. PMID: 12782134
FKBP52 is a component of the copper efflux machinery, and in so, may also promote neuroprotection from copper toxicity PMID: 15133031
Data report the crystal structures of two overlapped fragments of FK506-binding protein 52 and the heterocomplex of glucocorticoid receptors with heat-shock proteins 90. PMID: 15159550
FK506-binding proteins 51 and 52 differentially regulate dynein interaction and nuclear translocation of the glucocorticoid receptor PMID: 15591061
FKBP52 is an AR folding factor that has critically important physiological roles in some male reproductive tissues PMID: 15831525
results suggest that FKBP52 plays an important role in the regulation of TRPV5 and thus in the process of Ca(2+) reabsorption PMID: 16352746
FKBP52 is an essential regulator of PR-A action in the uterus. PMID: 16873445
FKBP52 may play a role in growth and development of male genitalia, since it is expressed in genital skin of prepubertal boys; however, alterations in the sequence and in expression of the FKBP4 gene are not a common cause of non-syndromic hypospadias. PMID: 17343741
phosphorylation of the FK linker appears to be an important regulatory determinant of FKBP52-mediated potentiation of steroid receptor activity PMID: 17717070
immunophilin ligands can protect neurons from Ca(2+)-induced cell death by modulating Ca(2+) channels and promote neurite outgrowth via FKBP52 binding PMID: 18162540
Data show that the loss of FKBP52 encourages the growth of endometriotic lesions with increased inflammation, cell proliferation, and angiogenesis. PMID: 18988805
Increased FKBP4 expression of correlated to HIV(+)major depressive disorder(MDD) but not to HIV without MDD PMID: 19199039
knockdown of FKBP4 gene, coding for the immunophilin FKBP52, inhibited cortisol-activated glucocorticoid receptor nuclear translocation PMID: 19545546
Resutls show that three of five autoantibodies, FKBP52, PPIA, and PRDX2, showed significantly increased reactivity in primary breast cancer and CIS compared with healthy controls. PMID: 19584157
FKBP52 mediates stimulus-dependent TRPC1 gating through isomerization, which is required for chemotropic turning of neuronal growth cones to midline axon guidance of commissural interneurons in the developing spinal cord. PMID: 19945390

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Subcellular Location

Cytoplasm, cytosol. Mitochondrion. Nucleus. Cytoplasm, cytoskeleton. Cell projection, axon.

Tissue Specificity

Widely expressed.

Database Links

HGNC: 3720

OMIM: 600611

KEGG: hsa:2288

STRING: 9606.ENSP00000001008

UniGene: Hs.524183

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Code	CSB-RA121108A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HEK293T whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: FKBP4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time：1min IHC image of CSB-RA121108A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of MCF-7 cell with CSB-RA121108A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing MCF-7 cells stained with CSB-RA121108A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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FKBP4 Recombinant Monoclonal Antibody

Product Details

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Target Background