GABRA5 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA559038A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Ntera-2 whole cell lysate, HEK293 whole cell lysate All lanes: GABA A Receptor alpha 5 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53 kDa Observed band size: 70 kDa IHC image of CSB-RA559038A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Target Background

Product Details

Uniprot No.

P31644

Target Names

GABRA5

Alternative Names

Gamma-aminobutyric acid receptor subunit alpha-5 (GABA(A) receptor subunit alpha-5), GABRA5

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human GABA A Receptor alpha 5

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

GABRA5 encodes the alpha-5 subunit of the GABA-A receptor, a ligand-gated chloride channel that mediates inhibitory neurotransmission throughout the central nervous system. This particular subunit shows enriched expression in the hippocampus, where it contributes to tonic inhibition and has been implicated in cognitive processes including learning and memory. Dysregulation of GABRA5-containing receptors has attracted significant research interest in contexts ranging from anxiety disorders to age-related cognitive decline, making reliable detection of this subunit essential for neuroscience investigations.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human GABA-A receptor alpha-5, offers the consistency and reproducibility that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant expression in rabbit host cells, researchers can expect minimal lot-to-lot variation across extended studies or collaborative projects requiring standardized reagents.

Validation testing demonstrates reliable performance across multiple applications. In western blot analysis, the antibody detects GABRA5 in Ntera-2 and HEK293 whole cell lysates at dilutions ranging from 1:500 to 1:5000. The observed band at approximately 70 kDa runs higher than the predicted 53 kDa molecular weight, a shift consistent with post-translational glycosylation commonly observed in membrane-associated receptor subunits. For tissue-based studies, immunohistochemical staining has been validated in paraffin-embedded human brain sections at dilutions of 1:50 to 1:200, with clear visualization achieved using standard antigen retrieval and polymer detection methods.

This antibody serves researchers investigating GABAergic signaling, synaptic physiology, and neurological disease mechanisms where precise localization and quantification of GABA-A receptor subunits advances experimental understanding.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Ligand-gated chloride channel subunit which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. May be involved in GABA-A receptor assembly, and GABA-A receptor immobilization and accumulation by gephyrin at the synapse.

Gene References into Functions

Low GABRA5 expression typified hyperproliferative tumors, and loss of taurine signaling correlated with reduced patient survival, suggesting this tumor suppressive mechanism operates in vivo. PMID: 29787571
Study found a significant reduction in [(11)C]Ro15 4513 binding in the nucleus accumbens in an opiate-dependent compared with the healthy control group. Study suggests that reduced GABA A receptor alpha5 levels in the nucleus accumbens are associated with addiction. PMID: 26876472
Report benzodiazepine derivative as GABA-site inhibitor of extra-synaptic GABAA alpha5 receptors. PMID: 26169564
Study found that a significant portion of GABAergic postsynaptic compartments contain the alpha5 GABAAR subunit, both in vitro and in vivo PMID: 25663431
This study showed that in male groups, the expression of GABRA5 was generally lower in schizophrenia cases compared to the control. PMID: 25660468
Findings provide genetic evidence for the involvement of the genes GABRB3 and GABRA5 in the susceptibility to panic disorder PMID: 24755890
1,2-dichlorohexafluorocyclobutane enhancement of GABRA5 activity is abolished by GABRB3 mutations. PMID: 25211390
The alpha5 nicotinic receptor GABAergic neurons form a link from the medial habenula to the serotonergic brain centers. PMID: 24227714
These results provide initial evidence of a GABAA alpha5 deficit in autism spectrum disorder and support further investigations of the GABA system in this disorder. PMID: 22546616
In subjects with schizophrenia, mean GABA (A) alpha5 rerceptor subunit mRNA expression is 15% lower in layer 4 of the dorsolateral prefrontal cortex. PMID: 20843900
These results suggest that the GABRA5 gene may confer susceptibility to bipolar I disorder. PMID: 15882799
GABA(A) receptor alpha5 subunit as a candidate gene for autism and bipolar disorder; observations suggest parent-of-origin and gain-of-function effects PMID: 17353214

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Subcellular Location

Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Protein Families

Ligand-gated ion channel (TC 1.A.9) family, Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily, GABRA5 sub-subfamily

Database Links

HGNC: 4079

OMIM: 137142

KEGG: hsa:2558

STRING: 9606.ENSP00000335592

UniGene: Hs.24969

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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