GOPC Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA210187A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate(30µg),PC-3 whole cell lysate(30µg), Hela whole cell lysate(30µg),THP-1 whole cell lysate(30µg),K562 whole cell lysate(30µg),Rat brain tissue lysate(20µg) All lanes: GOPC antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 51 kDa Observed band size: 59 kDa Exposure time：3min IHC image of CSB-RA210187A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA210187A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA210187A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9HD26

Target Names

GOPC

Alternative Names

CAL antibody; CFTR associated ligand antibody; CFTR-associated ligand antibody; dJ94G16.2 antibody; dJ94G16.2 PIST antibody; FIG antibody; Fused in glioblastoma antibody; Golgi associated PDZ and coiled coil motif containing antibody; Golgi associated PDZ and coiled coil motif containing protein antibody; Golgi-associated PDZ and coiled-coil motif-containing protein antibody; GOPC 1 antibody; GOPC antibody; GOPC_HUMAN antibody; GOPC1 antibody; OTTHUMP00000040403 antibody; PDZ protein interacting specifically with TC 10 antibody; PDZ protein interacting specifically with TC10 antibody; PDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibody; PDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibody; PIST antibody; Protein interacting specifically with Tc 10 antibody; Protein interacting specifically with Tc10 antibody

Species Reactivity

Human, Rat

Immunogen

A synthesized peptide derived from human GOPC

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4B10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

GOPC, also known as Golgi-associated PDZ and coiled-coil motif-containing protein, serves as a critical scaffolding protein involved in intracellular trafficking and protein sorting at the Golgi apparatus. This protein plays significant roles in regulating CFTR chloride channel trafficking, making it particularly relevant to cystic fibrosis research, while its identification as a fusion partner in certain glioblastomas has drawn attention in cancer biology studies.

This recombinant rabbit monoclonal antibody (clone 4B10) offers the consistency and reproducibility that demanding research protocols require. Generated against a synthetic peptide derived from human GOPC and produced through recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring your experimental results remain comparable across studies.

Validation data demonstrates robust performance across multiple applications. Western blot analysis detects GOPC in diverse human cell lines including HepG2, PC-3, HeLa, THP-1, and K562, as well as rat brain tissue lysate, confirming cross-species reactivity with human and rat samples. The observed band at 59 kDa runs slightly higher than the predicted 51 kDa molecular weight, likely reflecting post-translational modifications such as glycosylation or phosphorylation events common to Golgi-resident proteins. Immunohistochemistry validation in paraffin-embedded human glioma and stomach tissues demonstrates reliable tissue staining with citrate buffer antigen retrieval. Flow cytometry analysis in HeLa cells shows clear positive signal separation from isotype controls, supporting intracellular detection workflows.

Whether investigating Golgi trafficking mechanisms, CFTR-related pathways, or exploring GOPC's involvement in oncogenic fusions, this antibody provides a dependable tool for western blotting, immunohistochemistry, and flow cytometry applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.

Gene References into Functions

PIST, a Golgi-associated PDZ domain-containing protein, interacts with PCDH15 in the plasma membrane. PMID: 27867666
CAL can regulate the anterograde trafficking, the internalization as well as the signaling of CRFR1 via modulating the post-translational modifications that the receptor undergoes at the Golgi apparatus. PMID: 26115868
CAL overexpression retards B2AR expression in Golgi apparatus and reduces the receptor expression in the plasma membrane. PMID: 25876703
Six out of 65 (9%) BTC patients were positive for the FIG-ROS1 fusion, comprising two out of 14 (14%) gallbladder carcinoma (GBC) patients and four out of 25 (16%) extrahepatic cholangiocarcinoma (ECC) patients. PMID: 25231053
Our data suggest that PIST contributes to the fine-tuning of b1AR sorting both during biosynthetic and postendocytic trafficking PMID: 25614626
None of 268 gliomas analysed showed the FIG-ROS1(L) rearrangement. PMID: 24999209
Stereochemical preferences modulate affinity and selectivity among CAL PDZ domain protein variants that bind CFTR. PMID: 24210758
FIG-ROS fusion protein, which is found in a subset of intrahepatic cholangiocarcinoma patients, accelerates cholangiocarcinogenesis. PMID: 24154728
Data indicate that E3 ubiquitin ligase MARCH2 co-immunoprecipitated and co-localized with CAL and syntaxin 6 (STX6), and show the ubiquitination of CFTR by MARCH2. PMID: 23818989
The coiled-coil domain of PIST was then purified to homogeneity and crystallized at 293 K. Finally, X-ray diffraction data were collected to a resolution of 4.0 A from a crystal belonging to the hexagonal space group P6(2)22 or P6(4)22. PMID: 23545662
crystals of CAL PDZ domain diffracted to 1.4 A resolution PMID: 21543871
establish CAL inhibitors as founding members of a class of CFTR "stabilizers" specifically designed to reduce DeltaF508-CFTR post-endocytic breakdown PMID: 21105033
Syntaxin 6 and CAL mediate the degradation of the cystic fibrosis transmembrane conductance regulator PMID: 20130090
The FIG-ROS locus encodes for an in-frame fusion protein with a constitutively active kinase activity, suggesting that FIG-ROS may act as an oncogene. PMID: 12661006
CAL retains CFTR in the cell and targets CFTR for degradation. PMID: 14570915
modulates beta1AR intracellular trafficking PMID: 15358775
golgin-160 assosiates in an isoform-specific manner with the Golgi-associated protein PIST PMID: 15951434
intracellular sorting of the somatostatin receptor subtype 5 is regulated by interactions with PDZ domain proteins PIST/GOPC and PDZK1 PMID: 16012170
Our discoveries provide insight into the specific interaction of the GOPC PDZ domain with the C-terminal peptide of Nlg and also provide a general insight about the possible binding mode of the interaction of Nlg with other PDZ domain-containing proteins. PMID: 16882988
CAL is a negative regulator of DeltaF508-CFTR cell-surface expression PMID: 17158866
The cystic fibrosis-associated ligand (CAL) PDZ domain of the C-terminus of cystic fibrosis-associated conductance regulator (CFTR) is competitively involved with PDZ proteins Na+/H+ exchanger-3 regulatory factors NHERF1 and NHERF2. PMID: 18754678

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Involvement in disease

A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.

Subcellular Location

Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic density. Cell projection, dendrite.

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 17643

OMIM: 606845

KEGG: hsa:57120

STRING: 9606.ENSP00000357484

UniGene: Hs.191539

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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