Product Details

Uniprot No.

Target Names

Alternative Names

Acrogranin antibody; CLN11 antibody; GEP antibody; GP88 antibody; Granulin A antibody; Granulin B antibody; Granulin C antibody; Granulin D antibody; Granulin E antibody; Granulin epithelin antibody; Granulin F antibody; Granulin G antibody; Granulin-7 antibody; Granulins antibody; GRN antibody; GRN_HUMAN antibody; PC cell derived growth factor antibody; PCDGF antibody; PEPI antibody; PGRN antibody; Proepithelin antibody; Progranulin antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human GRN

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4E2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Progranulin, encoded by the GRN gene, serves as a critical regulator of cell growth, wound healing, and neuronal survival. This secreted glycoprotein has garnered significant research attention due to its involvement in frontotemporal dementia, where loss-of-function mutations lead to neurodegeneration, and its emerging roles in cancer biology where it can promote tumor growth and survival. Understanding progranulin expression and localization is essential for researchers investigating neurodegenerative mechanisms, inflammatory responses, and oncogenic pathways.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human progranulin, offers the consistency and reliability that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can compromise longitudinal studies and ensures reproducible results across your research program.

Validated across multiple applications, this antibody provides flexibility for diverse experimental approaches. Western blot analysis has confirmed detection in both human K562 and mouse NIH/3T3 cell lysates, demonstrating cross-species reactivity that expands your experimental options. The observed band at approximately 55 kDa differs from the predicted 64 kDa molecular weight, which likely reflects proteolytic processing of the progranulin precursor or differences in glycosylation state. Immunohistochemistry validation in human pancreatic cancer tissue reveals clear staining patterns, while immunofluorescence studies in HeLa cells and flow cytometry analysis of 786-O renal carcinoma cells further demonstrate the antibody's versatility across detection platforms.

Whether you are investigating progranulin's neuroprotective functions, exploring its role in tumor microenvironments, or characterizing its expression in inflammatory conditions, this antibody delivers the performance and reproducibility your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation. Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes. Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB. In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures. Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases. Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity.; Promotes proliferation of the epithelial cell line A431 in culture.; Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.; Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.

Gene References into Functions

progranulin reduction might be the cause of multiple proteinopathies due to the accelerating accumulation of abnormal proteins including TDP-43 proteinopathy, tauopathy and alpha-synucleinopathy. PMID: 28473694
Results suggest that serum PGRN levels are significantly higher in obese children in China and correlate significantly with obesity-related markers. Increased PGRN levels may be involved in the pathological mechanism of childhood obesity. PMID: 30151059
Progranulin has a direct effect on tumor growth, migration, and invasion of human colon cancer cells. It may serve as a potential therapeutic target for human colon cancer. PMID: 30060789
Accumulating evidence suggests that PGRN is essential for proper lysosomal function, but the precise mechanisms involved are not known. Here, we show that PGRN facilitates neuronal uptake and lysosomal delivery of prosaposin (PSAP), the precursor of saposin peptides that are essential for lysosomal glycosphingolipid degradation. PMID: 28541286
miR-29b-3p bound to PGRN in a direct manner. Transfection with si-PGRN correlated with elevated E-cadherin expression, decreased snail and vimentin expressions, as well as less-motivated cell proliferation and cell metastasis. The H19/miR-29b-3p/PGRN axis modulated epithelial-mesenchymal transition of NSCLC cells via Wnt/beta-catenin signaling. PMID: 29754471
SNPs in GRN are not related to ankylosing spondylitis susceptibility in a Chinese Han population. PMID: 29230494
Plasma progranulin levels predict the presence of GRN null mutations independent of proximity to symptoms and brain atrophy. PMID: 29146050
Serum progranulin may have potential to be used as a biomarker for the differentiation between early-onset pre-eclampsia and late-onset pre-eclampsia. PMID: 28574293
CHI3L1 may be a previously unrecognized biomarker for diagnosing Gaucher Diseases (GD) and for evaluating the therapeutic effects of new GD drug. PMID: 29396296
The increased serum progranulin concentrations were closely related to measures of adiposity, metabolic parameters, inflammatory marker and insulin resistance indices, suggesting that progranulin may be an excellent biomarker for obesity in childhood. PMID: 29176029
This work identifies PGRN as an activator of lysosomal cathepsin D activity, and suggests that decreased cathepsin D activity due to loss of PGRN contributes to both FTD and NCL pathology in a dose-dependent manner. PMID: 29036611
MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN. PMID: 28609022
Results provide evidence that progranulin destabilizes sortilin via ubiquitination and lysosomal degradation to override the negative modulatory functions of sortilin thereby assuring the sustainable pro-tumorigenic actions of progranulin in castration-resistant prostate cancer. PMID: 28433812
PGRN is a regulator of tumorigenesis because it stimulates cell proliferation, migration, invasion, angiogenesis, malignant transformation, resistance to anticancer drugs, and immune evasion. [review] PMID: 29116422
PGRN regulated its own expression in a positive feedback loop via the AKT and ERK signaling pathways. PMID: 29039535
PGRN is involved in lysosomal homeostasis and lipid metabolism. PMID: 28903038
High serum GP88 concentrations are associated with poor prognosis in patients with DLBCL PMID: 28823651
This study showed that endogenous levels of multiple GRNs are haploinsufficient in FTD-GRN patient fibroblasts and frontal cortex, mirroring full-length PGRN. PMID: 28828399
HSPA5 (GRP78) and GEP were identified to interact. Clinical analysis showed that expression of GRP78 was up-regulated in hepatocellular carcinoma tumor and correlated with GEP expression. PMID: 28601093
the expression of PGRN was significantly upregulated in Osteonecrosis of the Femoral Head patients' articular cartilage, and recombinant PGRN could promote expression of aggrecan and collagen II and the activation of ERK1/2. PMID: 28247166
White matter hyperintensities are seen only in GRN mutation carriers in fronto-temporal dementia. PMID: 28529873
The study provides solid evidence that there is the interaction between GRN A and ENO1 and the interaction is responsible for the effects of GRN A on glucose uptake as well as cancer cell migration and invasion. PMID: 28415822
This study ascertained that PGRN expression in airway epithelial cells inhibits CS-induced apoptosis, presumably through regulation of the ER stress response and MAPK activation. PMID: 28273689
This study describe a novel GRN, p.(Tyr229*) mutation, resulting in haploinsufficiency of GRN and a severe neuropathologic FTLD phenotype. PMID: 27767988
This meta-analysis supported the T allele of rs5848 within progranulin, as a risk factor for Alzheimer's Disease. PMID: 26820675
Our case report further corroborates the notion that, in addition to FTD, progranulin may be involved in the neurobiology of bipolar disorder type 1, and suggests to screen patients with late-onset bipolar disorder for GRN mutations. PMID: 28664756
GRN SNPs likely influence disease course and relapse recovery in multiple sclerosis. PMID: 26447062
This study revealed no patients presenting with typical clinical PSPS to have evidence of GRN mutations producing TDP-43 pathology. PMID: 27163816
This study reported a novel pathogenic splice site mutation in GRN (c.709-2 A>T) causative for FTD and we show that 1) GRN LoF mutations are a rare cause of FTD in this Italian cohort. PMID: 27258413
progranulin expression is critical for tumor growth in vivo, in both xenograft and orthotopic tumor models PMID: 27220888
results revealed an important role of NF-kappaB signalling in PGRN-associated frontotemporal lobar degeneration-DNA-binding protein 43 and confirm that PGRN can bind to TNF-alpha receptors regulating the expression of WNT5A, suggesting novel targets for treatment of frontotemporal lobar degeneration-DNA-binding protein linked to GRN mutations. PMID: 26624524
These results suggest an important role of Wnt activation inducing cell cycle disturbance-mediated neuronal loss in the pathogenesis of PGRN deficiency-linked frontotemporal lobar degeneration with TDP-43 protein inclusions. PMID: 26676574
Study demonstrate that PGRN interacts with the lysosomal protease CTSD and maintains its proper activity in vivo. Therefore, by regulating CTSD activity, PGRN may modulate protein homeostasis. This could potentially explain the TDP-43 aggregation observed in frontotemporal lobar degeneration with GRN mutations. PMID: 28493053
Data indicate significant correlation of granulin-epithelin precursor (GEP) with beta-catenin in hepatocellular carcinoma (HCC) cohort. PMID: 26942873
We identified 3 novel GRN mutations (p.Q130X, p.317Afs*12, and p.K259Afs*23) in patients diagnosed with nonfluent-variant PPA or behavioral-variant FTD. The frequency of GRN mutations was 9.6% and that of MAPT mutations was 7.1%. Among familial cases of FTD, the frequency of GRN mutations was 31.5% and that of MAPT mutations was 10.5% in Brazil. PMID: 27082848
Missense mutation in GRN gene affecting RNA splicing and plasma progranulin level in a family affected by frontotemporal lobar degeneration. PMID: 28285794
existence of a founder effect for C157KfsX97 mutation in patients with frontotemporal lobar degeneration inSouthern Italy. PMID: 27814992
GRN gene deletion is genetic etiology of familial frontotemporal dementia. PMID: 28153380
Thisstudy has demonstrated the effect of inflammatory cytokine on cartilage graft as well as the protective role of PGRN on this graft. Without the anti-inflammatory effect from PGRN, hyaline cartilaginous extracellular matrices in living hyaline cartilage graft constructs could be easily remodeled into fibrotic or mineralized tissue and would no longer be able to fulfil the role of a cartilage graft. PMID: 27465877
This study demonstrated that the GRN mutation carriers showed significant frontoparietal hypoperfusion compared with controls at follow-up. PMID: 27625986
The GRN risk SNP (rs5848) was associated with variation in detected levels of CSF proteins previously implicated in CNS inflammation. The same GRN risk allele was also associated with increased CSF tau which may indicate directly related neurodegenerative changes. PMID: 28189700
Granulin is deposited in specific brain regions that differ between Alzheimer's disease patients and Frontotemporal lobar degeneration patients. PMID: 29044416
Mutation of GRN is a common cause of FTD in mainland China. PMID: 27311648
the influence of the p.A152T MAPT variant on the clinical and neuropathological features of these Basque GRN families, is reported. PMID: 28594853
Results indicate that central nervous progranulin reduction is not restricted to the relatively rare cases of frontotemporal dementia caused by progranulin gene (GRN) loss of function mutations, but also contributes to the more common GRN-negative forms of frontotemporal dementia. PMID: 27760429
a complete screening for mutations in MAPT, GRN and C9ORF72 genes revealed a frequency of 5.4% of pathogenic mutations in a random cohort of 93 Turkish index patients with dementia PMID: 27632209
This Progress article reviews recent advances in progranulin biology emphasizing its roles in lysosomal function and brain innate immunity, and outlines future avenues of investigation that may lead to new therapeutic approaches for neurodegeneration--{REVIEW} PMID: 28435163
serum progranulin levels had a positive correlation with pulmonary function in patients with asthma and a negative correlation with blood neutrophil counts. PMID: 28073702
Progranulin (PGRN), a 593-amino-acid autocrine growth factor has multiple functions involved in cell proliferation, wound healing, neurodegeneration, insulin resistance, and tumorigenesis. PMID: 27149013
progranulin is affected by a decrease in eGFR, being at a higher concentration in serum and lower in urine of DKD patients with T2DM and eGFR <60 mL/min/1.73m2. It is also associated with markers of obesity and inflammation PMID: 27776152

Hide All

Involvement in disease

Ubiquitin-positive frontotemporal dementia (UP-FTD); Ceroid lipofuscinosis, neuronal, 11 (CLN11)

Subcellular Location

Secreted. Lysosome.

Protein Families

Granulin family

Tissue Specificity

In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.

Database Links

HGNC: 4601

OMIM: 138945

KEGG: hsa:2896

STRING: 9606.ENSP00000053867

UniGene: Hs.514220

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA079244A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: K562 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: Progranulin antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 64 kDa Observed band size: 55 kDa Exposure time：3min IHC image of CSB-RA079244A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA079244A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing 786-O cells stained with CSB-RA079244A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

GRN Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background