GSS Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA188887A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg), Hela whole cell lysate(30µg), PC-3 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), A431 whole cell lysate(30µg), Mouse liver tissue lysate(30µg), Rat liver tissue lysate(30µg), Mouse lung tissue lysate(30µg) All lanes: GSS antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time：2min Immunofluorescence staining of A549 cell with CSB-RA188887A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing A431 cells stained with CSB-RA188887A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P48637

Target Names

GSS

Alternative Names

Glutathione synthetase (GSH synthetase) (GSH-S) (EC 6.3.2.3) (Glutathione synthase), GSS

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human GSS protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2H9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Glutathione synthetase catalyzes the final step in glutathione biosynthesis, converting gamma-glutamylcysteine and glycine into the essential antioxidant glutathione. This enzyme plays a central role in cellular defense against oxidative stress, xenobiotic detoxification, and maintenance of redox homeostasis. Researchers investigating metabolic disorders, oxidative damage pathways, and cancer metabolism frequently require reliable tools to monitor GSS expression across experimental models.

This recombinant monoclonal antibody, generated from clone 2H9 against a synthetic peptide derived from human GSS, offers the consistency and reproducibility that demanding research protocols require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, you can expect lot-to-lot uniformity that supports longitudinal studies and reproducible data across your research program.

Validation testing demonstrates robust performance across multiple experimental platforms. Western blot analysis detects a clean band at the predicted 52 kDa molecular weight across diverse human cell lines including HEK293, HeLa, PC-3, HepG2, SH-SY5Y, and A431 cells at dilutions ranging from 1:500 to 1:2000. Cross-species reactivity extends to mouse and rat samples, with confirmed detection in mouse liver and lung tissue as well as rat liver tissue, providing flexibility for translational studies using rodent models. Immunofluorescence staining in A549 cells reveals cytoplasmic localization patterns consistent with the enzyme's known subcellular distribution, while flow cytometry analysis in A431 cells demonstrates clear separation from isotype controls.

Whether you are characterizing glutathione metabolism in cancer cell lines, investigating oxidative stress responses in tissue samples, or examining GSS expression across species, this antibody provides the validated performance and application versatility to support your experimental objectives.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner. Glutathione (gamma-glutamylcysteinylglycine, GSH) is the most abundant intracellular thiol in living aerobic cells and is required for numerous processes including the protection of cells against oxidative damage, amino acid transport, the detoxification of foreign compounds, the maintenance of protein sulfhydryl groups in a reduced state and acts as a cofactor for a number of enzymes.

Gene References into Functions

Data suggest that the potential of clusterin and glutathione synthetase (GSH-S) as platelet biomarkers for early detection of colorectal cancer (CRC) could improve existing screening modalities in clinical application. PMID: 28849249
Mutation in Glutathione Synthase gene is associated with chronic metabolic acidosis in glutathione synthetase deficiency. PMID: 26669244
Four SNPs (rs7265992, rs6060124, rs7260770, and rs4911455) in GSS were significantly associated with bladder cancer recurrence after transurethral resection and BCG treatment. PMID: 25851338
In this study, clinical, biochemical, and genetic aspects of five Chinese 5-oxoprolinuria patients with OPLAH or GSS gene mutations were investigated. PMID: 25851806
Studied the role of protein-protein interactions in the structural stability, activity and allostery of enzymes using the obligate homodimer human glutathione synthetase as an ideal model. PMID: 25070563
The findings indicate that Asp458 is essential for hGS catalysis and that it impacts the allostery of hGS. PMID: 21771585
These results imply that residues V44 and V45 are integral to the stability of human glutathione synthetase. PMID: 21683691
We have shown that susceptibility to health effects of air pollution on lung function growth is associated with genetic variation in the GSS gene PMID: 20802163
Single-nucleotide polymorphism in glutathione synthetase is associated with small-cell lung cancer. PMID: 20439344
This research indicates that Gly369 and Gly370 have essential roles in hGS, while Gly371 has a lesser involvement. PMID: 20800579
the cause of cellular ATP depletion in nephrotic cystinosis may be the futile cycle, formed between two ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine synthetase and 5-oxoprolinase PMID: 20413906
A novel alternative splicing variant (ASV) of the GSS gene was identified in 10 human normal tissues and five human cancer cell lines. PMID: 19672693
Glutathione synthetase deficiency: is gamma-glutamylcysteine accumulation a way to cope with oxidative stress in cells with insufficient levels of glutathione? PMID: 12638941
analysis of conserved residues of human glutathione synthetase PMID: 14990577
The cloning and characterization of a 2.2 kb 5'-flanking region of the human glutathione synthetase gene is reported. PMID: 15890065
glutathione synthetase autosomal mutations result in glutathione synthetase deficiency, which may cause progressive retinal dystrophy with hyperpigmentations and maculopathy [case report] PMID: 17206463
Glutathione synthase expression may indicate better survival in early stage adenocarcinoma of the lung, and manipulation of glutathione synthase may be a potential basis for treatment of some non-small cell lung cancers. PMID: 17234469
Severe glutathione synthetase deficiency is associated with progressive retinal dystrophy of the rod-cone type, affecting the central retina with advanced macular edema in adulthood. PMID: 19111905

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Involvement in disease

Glutathione synthetase deficiency (GSS deficiency); Glutathione synthetase deficiency of erythrocytes (GLUSYNDE)

Protein Families

Eukaryotic GSH synthase family

Database Links

HGNC: 4624

OMIM: 231900

KEGG: hsa:2937

STRING: 9606.ENSP00000216951

UniGene: Hs.82327

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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