Recombinant Human Glutathione synthetase (GSS)

Code CSB-YP009969HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP009969HU
MSDS
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Source E.coli
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Code CSB-EP009969HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009969HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP009969HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GSS
Uniprot No.
Alternative Names
epididymis secretory sperm binding protein Li 64p; epididymis secretory sperm binding protein Li 88n; Glutathione synthase; Glutathione synthetase; GSH S; GSH synthetase; GSH-S; GSHB_HUMAN; GSHS; GSS; HEL-S-64p; HEL-S-88n; MGC14098; OTTHUMP00000030711
Species
Homo sapiens (Human)
Expression Region
2-474
Target Protein Sequence
ATNWGSLLQ DKQQLEELAR QAVDRALAEG VLLRTSQEPT SSEVVSYAPF TLFPSLVPSA LLEQAYAVQM DFNLLVDAVS QNAAFLEQTL SSTIKQDDFT ARLFDIHKQV LKEGIAQTVF LGLNRSDYMF QRSADGSPAL KQIEINTISA SFGGLASRTP AVHRHVLSVL SKTKEAGKIL SNNPSKGLAL GIAKAWELYG SPNALVLLIA QEKERNIFDQ RAIENELLAR NIHVIRRTFE DISEKGSLDQ DRRLFVDGQE IAVVYFRDGY MPRQYSLQNW EARLLLERSH AAKCPDIATQ LAGTKKVQQE LSRPGMLEML LPGQPEAVAR LRATFAGLYS LDVGEEGDQA IAEALAAPSR FVLKPQREGG GNNLYGEEMV QALKQLKDSE ERASYILMEK IEPEPFENCL LRPGSPARVV QCISELGIFG VYVRQEKTLV MNKHVGHLLR TKAIEHADGG VAAGVAVLDN PYPV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner. Glutathione (gamma-glutamylcysteinylglycine, GSH) is the most abundant intracellular thiol in living aerobic cells and is required for numerous processes including the protection of cells against oxidative damage, amino acid transport, the detoxification of foreign compounds, the maintenance of protein sulfhydryl groups in a reduced state and acts as a cofactor for a number of enzymes.
Gene References into Functions
  1. Data suggest that the potential of clusterin and glutathione synthetase (GSH-S) as platelet biomarkers for early detection of colorectal cancer (CRC) could improve existing screening modalities in clinical application. PMID: 28849249
  2. Mutation in Glutathione Synthase gene is associated with chronic metabolic acidosis in glutathione synthetase deficiency. PMID: 26669244
  3. Four SNPs (rs7265992, rs6060124, rs7260770, and rs4911455) in GSS were significantly associated with bladder cancer recurrence after transurethral resection and BCG treatment. PMID: 25851338
  4. In this study, clinical, biochemical, and genetic aspects of five Chinese 5-oxoprolinuria patients with OPLAH or GSS gene mutations were investigated. PMID: 25851806
  5. Studied the role of protein-protein interactions in the structural stability, activity and allostery of enzymes using the obligate homodimer human glutathione synthetase as an ideal model. PMID: 25070563
  6. The findings indicate that Asp458 is essential for hGS catalysis and that it impacts the allostery of hGS. PMID: 21771585
  7. These results imply that residues V44 and V45 are integral to the stability of human glutathione synthetase. PMID: 21683691
  8. We have shown that susceptibility to health effects of air pollution on lung function growth is associated with genetic variation in the GSS gene PMID: 20802163
  9. Single-nucleotide polymorphism in glutathione synthetase is associated with small-cell lung cancer. PMID: 20439344
  10. This research indicates that Gly369 and Gly370 have essential roles in hGS, while Gly371 has a lesser involvement. PMID: 20800579
  11. the cause of cellular ATP depletion in nephrotic cystinosis may be the futile cycle, formed between two ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine synthetase and 5-oxoprolinase PMID: 20413906
  12. A novel alternative splicing variant (ASV) of the GSS gene was identified in 10 human normal tissues and five human cancer cell lines. PMID: 19672693
  13. Glutathione synthetase deficiency: is gamma-glutamylcysteine accumulation a way to cope with oxidative stress in cells with insufficient levels of glutathione? PMID: 12638941
  14. analysis of conserved residues of human glutathione synthetase PMID: 14990577
  15. The cloning and characterization of a 2.2 kb 5'-flanking region of the human glutathione synthetase gene is reported. PMID: 15890065
  16. glutathione synthetase autosomal mutations result in glutathione synthetase deficiency, which may cause progressive retinal dystrophy with hyperpigmentations and maculopathy [case report] PMID: 17206463
  17. Glutathione synthase expression may indicate better survival in early stage adenocarcinoma of the lung, and manipulation of glutathione synthase may be a potential basis for treatment of some non-small cell lung cancers. PMID: 17234469
  18. Severe glutathione synthetase deficiency is associated with progressive retinal dystrophy of the rod-cone type, affecting the central retina with advanced macular edema in adulthood. PMID: 19111905

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Involvement in disease
Glutathione synthetase deficiency (GSS deficiency); Glutathione synthetase deficiency of erythrocytes (GLUSYNDE)
Protein Families
Eukaryotic GSH synthase family
Database Links

HGNC: 4624

OMIM: 231900

KEGG: hsa:2937

STRING: 9606.ENSP00000216951

UniGene: Hs.82327

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