GTPBP4 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA781541A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: MCF7 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), A549 whole cell lysate(30µg), A2780 whole cell lysate(30µg), COLO205 whole cell lysate(30µg) All lanes:GTPBP4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 74 kDa Observed band size: 80 kDa Exposure time：1min IHC image of CSB-RA781541A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing HepG2 cells stained with CSB-RA781541A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9BZE4

Target Names

GTPBP4

Alternative Names

Chronic renal failure gene protein antibody; CRFG antibody; G protein binding protein CRFG antibody; GTP binding protein 4 antibody; GTP-binding protein NGB antibody; Gtpbp4 antibody; NGB antibody; NOG1 antibody; NOG1_HUMAN antibody; Nucleolar GTP binding protein 1 antibody; Nucleolar GTP-binding protein 1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human GTPBP4 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8F2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Involved in the biogenesis of the 60S ribosomal subunit.

Gene References into Functions

GTPBP4 promotes colorectal cancer metastasis by disrupting actin cytoskeleton, which is mediated by the reduced RhoA activity.Up-regulation of GTPBP4 in colorectal carcinoma is responsible for tumor metastasis. PMID: 27720713
NGB is a tumor suppressor that regulates and requires merlin to suppress cell proliferation PMID: 17210637

Subcellular Location

Nucleus, nucleolus.

Protein Families

TRAFAC class OBG-HflX-like GTPase superfamily, OBG GTPase family, NOG subfamily

Database Links

HGNC: 21535

KEGG: hsa:23560

STRING: 9606.ENSP00000354040

UniGene: Hs.215766

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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