Product Details

Uniprot No.

Target Names

HLTF

Alternative Names

DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody; Helicase like transcription factor antibody; Helicase-like transcription factor antibody; HIP116 antibody; HIP116A antibody; HLTF 1 antibody; Hltf antibody; HLTF_HUMAN antibody; HLTF1 antibody; p113 antibody; RING finger protein 80 antibody; RNF80 antibody; SMARC A3 antibody; SMARCA 3 antibody; SMARCA3 antibody; SNF2-like 3 antibody; SNF2L3 antibody; Sucrose nonfermenting protein 2 like 3 antibody; Sucrose nonfermenting protein 2-like 3 antibody; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 3 antibody; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a3 antibody; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3 antibody; ZBU 1 antibody; ZBU1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human HLTF protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

21D10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

HLTF (Helicase-like transcription factor) serves as a critical chromatin remodeling enzyme belonging to the SWI/SNF family, playing essential roles in DNA damage response, replication fork stability, and transcriptional regulation. This multifunctional protein, also known as SMARCA3, possesses both helicase and E3 ubiquitin ligase activities, making it a compelling target for researchers investigating genome maintenance mechanisms, cancer biology, and epigenetic regulation.

This recombinant rabbit monoclonal antibody (clone 21D10) offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human HLTF, the antibody undergoes affinity chromatography purification to ensure high specificity. Because recombinant production relies on defined genetic sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot consistency that supports longitudinal studies and reproducible results across experiments.

Validation studies demonstrate reliable performance across multiple detection platforms. Western blot analysis successfully detects HLTF in MCF-7 breast cancer, A549 lung adenocarcinoma, and A431 epidermoid carcinoma cell lysates, with observed bands at approximately 120 kDa—slightly higher than the predicted 114 kDa molecular weight, likely reflecting post-translational modifications such as glycosylation or phosphorylation common to nuclear proteins. Immunohistochemistry staining in paraffin-embedded human lung cancer tissue confirms utility for tissue-based studies, while flow cytometry analysis in MCF-7 cells demonstrates clear positive signal separation from isotype controls, enabling intracellular protein quantification.

With validated applications spanning ELISA, western blotting, immunohistochemistry, and flow cytometry, this antibody provides flexibility for researchers exploring HLTF's contributions to chromatin dynamics, DNA repair pathways, and tumor suppression mechanisms across diverse experimental contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters. These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.

Gene References into Functions

Data suggest that HIV-1 vpr mediates polyubiquitination of HLTF by directly loading it onto C-terminal WD40 domain of DCAF1 in complex the CRL4, an E3 ubiquitin ligase. (vpr = vpr gene product of Human immunodeficiency virus 1; HLTF = human helicase like transcription factor; DCAF1 = human Vpr (HIV-1) binding protein; CRL4 = human E3 ubiquitin ligase CRL4) PMID: 29079575
A low WT HLTF expression with a high I21R HLTF expression is associated with a poor disease-free survival PMID: 29661164
the present study does not provide any strong evidence that PAI-1 gene variants are implicated in the risk of DR or the development of DR during T2DM course. PMID: 28632032
A large number of SNF2 family, DNA and ATP-dependent motor proteins are needed during transcription, DNA replication, and DNA repair to manipulate protein-DNA interactions and change DNA structure. SMARCAL1, ZRANB3, and HLTF are three related members of this family with specialized functions that maintain genome stability during DNA replication. [review] PMID: 28954549
depletion of SMARCAL1, a SNF2-family DNA translocase that remodels stalled forks, restores replication fork stability and reduces the formation of replication stress-induced DNA breaks and chromosomal aberrations in BRCA1/2-deficient cells. In addition to SMARCAL1, other SNF2-family fork remodelers, including ZRANB3 and HLTF, cause nascent DNA degradation and genomic instability PMID: 29053959
HIV-1 vpr reprograms CRL4(DCAF1) E3 to direct HLTF for proteasome-dependent degradation independent from previously reported Vpr interactions with base excision repair enzyme uracil DNA glycosylase (UNG2) and crossover junction endonuclease MUS81, which Vpr also directs for degradation via CRL4(DCAF1) E3. PMID: 27335459
Fasudil reduced LPS-mediated TF and PAI-1 expression and activity in PBMCs. These effects may partially be relevant to the clinical benefits of fasudil in the treatment of CAPD patients. PMID: 27756191
HLTF is degraded in lymphocytic cells and macrophages infected with Vpr-expressing HIV-1. Our results reveal a previously unidentified strategy for HIV-1 to antagonize DNA repair in host cells. PMID: 27114546
HLTF expression is altered in various cancers via two mechanisms: gene silencing through promoter hypermethylation or alternative mRNA splicing, which leads to the expression of truncated proteins that lack DNA repair domains. [review] PMID: 26472339
HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. PMID: 26350214
Findings indicate a mechanism of helicase-like transcription factor HLTF-mediated fork reversal and suggest the requirement for distinct fork remodeling activities in the cell. PMID: 26051180
These results suggest that the HIRAN domain functions as a sensor to the 3'-end of the primer strand at the stalled replication fork and that the domain facilitates fork regression. PMID: 25858588
Study demonstrates a correlation between HLTF expression level and thyroid neoplastic progression where three truncated forms are detected in thyroid carcinoma. PMID: 25005870
We were able to provide evidence that methylation of HLTF and especially HPP1 detected in serum is strongly correlated with cell death in CRC using LDH as surrogate marker PMID: 24708595
Results demonstrate that loss of HLTF function promotes the malignant transformation of intestinal or colonic adenomas to carcinomas by inducing genomic instability. PMID: 22452792
results delineate a previously unknown USP7-HLTF-PCNA molecular network controlling DNA damage response PMID: 21845734
HLTF can displace a broad spectrum of proteins such as replication protein A (RPA), PCNA, and replication factor C (RFC), thereby providing the first example for a protein clearing activity at the stalled replication fork. PMID: 21795603
HLTF and SHPRH suppress mutagenesis in a damage-specific manner, preventing mutations induced by UV rays and methyl methanesulfonate. PMID: 21396873
results suggest that HLTF is involved in DNA repair and apoptosis in cancer cells, which might represent a target for gene therapies of human cancer PMID: 20535496
this is the first report identifying the regulatory mechanism of HLTF by CHFR, suggesting that CHFR-mediated downregulation of HLTF may help protect against cancer. PMID: 20388495
HLTF can promote error-free replication of damaged DNA and support a role for HLTF in preventing mutagenesis and carcinogenesis, providing thereby for its potential tumor suppressor role. PMID: 19948885
These results suggest that HLTF promoter hypermethylation is frequently demonstrated in human gastric cancer, and inactivation of HLTF or the chromatin-remodeling complex may play a crucial role in gastric carcinogenesis. PMID: 12766908
Acetylation of histones H3 and H4 in the 5' CpG island of the HLTF gene were inversely associated with DNA methylation status. PMID: 12901794
Methylation helicase-like transcription factor is associated with advanced esophageal squamous cell carcinoma PMID: 17094449
HLTF activation was recently linked to the initial steps of carcinogenesis in an experimental model of estrogen-induced kidney tumor. [REVIEW] PMID: 18034322
HLTF physically interacts with the Rad6-Rad18 and Mms2-Ubc13 ubiquitin-conjugating enzyme complexes and promotes the Lys-63-linked polyubiquitination of proliferating cell nuclear antigen at its Lys-164 residue. PMID: 18316726
Splice arrays and RT-PCR showed that although most splice variants in RUSH and ATP11B are conserved in human and rabbit, the RFBP isoform is specific to rabbit. PMID: 18584949
HLTF and SHPRH are functional homologues of yeast Rad5 that cooperatively mediate PCNA polyubiquitination and maintain genomic stability. PMID: 18719106
There is an association between the presence of helicase-like transcription factor and neoplastic progression of hypopharyngeal and laryngeal squamous cell carcinomas. PMID: 18825407
Hypopharyngeal squamous cell carcinomas presenting high levels of HLTF have a worse prognosis. PMID: 19614770
biochemical characterisation of cellular HLTF is described. PMID: 19723507
HLTF/SMARCA3, a member of SWI/SNF family, is a common target for methylation and epigenetic gene silencing in colon cancer and a candidate colon cancer suppressor gene. PMID: 11904375
SMARCA3 is the human gene for RUSH transcription factors (U66564, U66565; Mol Endocrinol, 1996, 10:1335-1349). A binding partner has been cloned and characterized (AF236061; JBC, 2001, 276:3641-3649). PMID: 11058586

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Subcellular Location

Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm.

Protein Families

SNF2/RAD54 helicase family, RAD16 subfamily

Tissue Specificity

Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.

Database Links

HGNC: 11099

OMIM: 603257

KEGG: hsa:6596

STRING: 9606.ENSP00000308944

UniGene: Hs.3068

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Code	CSB-RA045000A0HU
Size	US$210
	Order now
Image	Western Blot , Positive WB detected in:MCF7 whole cell lysate(30µg), A549 whole cell lysate(30µg), A431 whole cell lysate(30µg) All lanes: HLTF antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 114 kDa Observed band size: 120 kDa Exposure time：10s IHC image of CSB-RA045000A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing MCF-7 cells stained with CSB-RA045000A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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HLTF Recombinant Monoclonal Antibody

Product Details

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Target Background