HMGB2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA230291A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HL-60 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), THP-1 whole cell lysate(30µg) All lanes: HMGB2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 24 kDa Observed band size: 28 kDa Exposure time：2min IHC image of CSB-RA230291A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA230291A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of SH-SY5Y cell with CSB-RA230291A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA230291A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P26583

Target Names

HMGB2

Alternative Names

High mobility group protein B2 (High mobility group protein 2) (HMG-2), HMGB2, HMG2

Species Reactivity

Human

Immunogen

A synthesized peptide from human HMGB2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11B2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

HMGB2 (High Mobility Group Box 2) is a non-histone chromosomal protein that plays essential roles in chromatin architecture, transcriptional regulation, and DNA repair processes. As a member of the HMG protein family, HMGB2 facilitates nucleosome remodeling and serves as a critical regulator of gene expression during development and cellular differentiation. Its involvement in inflammatory responses and emerging connections to cancer biology make HMGB2 an important target for researchers investigating chromatin dynamics, immune signaling, and oncogenic mechanisms.

This recombinant rabbit monoclonal antibody (clone 11B2) offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human HMGB2 and produced through recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring reliable results across extended studies and collaborative projects.

Validation across multiple platforms demonstrates broad experimental utility. Western blot analysis detects HMGB2 in diverse human cell lines including HL-60, Jurkat, COLO-205, HEK293, and THP-1, with optimal performance at dilutions between 1:500 and 1:2000. The observed band at 28 kDa, slightly higher than the predicted 24 kDa molecular weight, likely reflects post-translational modifications such as acetylation or phosphorylation commonly associated with HMGB proteins. Immunohistochemistry applications have been validated in human lung cancer and testis tissues, while immunofluorescence staining in SH-SY5Y neuroblastoma cells reveals clear nuclear localization patterns. Flow cytometry analysis in Jurkat cells further extends its utility for single-cell studies.

This antibody supports research in chromatin biology, transcriptional regulation, inflammatory signaling, and cancer research where HMGB2 expression patterns provide mechanistic insights.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V(D)J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity. Involved in V(D)J recombination by acting as a cofactor of the RAG complex: acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Proposed to be involved in the innate immune response to nucleic acids by acting as a promiscuous immunogenic DNA/RNA sensor which cooperates with subsequent discriminative sensing by specific pattern recognition receptors. In the extracellular compartment acts as a chemokine. Promotes proliferation and migration of endothelial cells implicating AGER/RAGE. Has antimicrobial activity in gastrointestinal epithelial tissues. Involved in inflammatory response to antigenic stimulus coupled with proinflammatory activity. Involved in modulation of neurogenesis probably by regulation of neural stem proliferation. Involved in articular cartilage surface maintenance implicating LEF1 and the Wnt/beta-catenin pathway.

Gene References into Functions

HMBG2 overexpression promotes ischemia/reperfusion-induced cell apoptosis through activating the JNK1/2-NF-kappaBp65 signaling in AC16 cardiomyocytes. PMID: 30119172
Authors demonstrate that HMGB2 transcription is repressed by p21 during radiation-induced senescence through the ATM-p53-p21 DNA damage signaling cascade. The loss of p21 abolished the downregulation of HMGB2 caused by ionizing radiation, and the conditional induction of p21 was sufficient to repress the transcription of HMGB2. PMID: 29487276
identified the oncogene HMGB2 as a downstream target of miR-130a by using luciferase and western blot assays. Knockdown of HMGB2 mimicked the effect of miR-130a in glioma cells. PMID: 28851665
Serum HMGB2 levels were associated with in-stent restenosis in patients. PMID: 28183701
results establish HMGB2 as a novel master regulator that orchestrates senescence-associated secretory phenotype. PMID: 27799366
Data indicate the function of high mobility group box 2 (HMGB2) in glycolytic control in pancreatic cancer. PMID: 28069585
data demonstrate a reciprocal relationship between Hmgb2 and Ctcf in controlling aspects of chromatin structure and gene expression. PMID: 27226577
Lrp1-antisense directly binds to high-mobility group box 2 (Hmgb2) and inhibits the activity of Hmgb2 to enhance Srebp1a-dependent transcription of Lrp1. PMID: 25937287
Data show that odulation of RNA helicase DDX18 directly affects growth of tamoxifen-resistant cells, suggesting that it may be a critical downstream effector of the estrogen receptors (ERs) and high mobility group box 2 (HMGB2) complex. PMID: 25284587
siRNA-mediated silencing of HMGB2 increased the sensitivity of the head and neck squamous cell carcinoma cell lines to cisplatin and 5-fluorouracil. PMID: 25327479
The present data suggest that HMGB2 expression is a significant prognostic factor for glioblastoma and might play an important role in cell invasion. PMID: 23828241
HMGB2 is necessary to protect colorectal cancer cells from DNA damage and efficient DNA repair and p53-mediated downregulation is a critical mechanism of modulating HMGB2 expression. PMID: 23255232
Transient local HMGB2-DNA contacts dominate the DNA-bending mechanism used by HMGB proteins to increase DNA flexibility. PMID: 23143110
The age-related loss of HMGB2 in articular cartilage may represent a mechanism responsible for the decline in adult cartilage stem cell populations. PMID: 21890638
The results can partly be explained by altered cell proliferations by HMGB2 associated with the antiapoptotic pathway. PMID: 20851854
Our findings collectively suggest that HMGB2 could stabilize p53 by interfering with E6/E6AP-mediated p53 degradation in HPV-positive HeLa cells. PMID: 20036050
HMGB2 potentiates GATA-1-dependent transcription of GFI1B by Oct-1 and thereby controls erythroid differentiation. PMID: 19965638
HMG2 interacts with the nucleosome assembly protein SET and is a target of the cytotoxic T-lymphocyte protease granzyme A. PMID: 11909973
binds specifically to the first AT-rich region flanking the hypersensitive site 2 core sequence of the human beta-globin gene locus control region PMID: 12555809
biophysical analysis of HMG domain from human HMGB2 binding to DNA PMID: 15833996
HMG2 was determined in humans. PMID: 17406091
single and double box proteins increase DNA flexibility and stability, albeit both effects are achieved at much lower protein concentrations for the double box. PMID: 17964600
In human and murine cartilage, there is an aging-related loss of HMGB2 expression, ultimately leading to its complete absence. PMID: 19139395
An isolated HMG box A domain from human HMGB2 does not enhance DNA flexibility. Substitution of a small number of cationic residues from the N-terminal leader of a functional yeast box B protein, Nhp6Ap, confers the ability to enhance DNA flexibility. PMID: 19236006

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Subcellular Location

Nucleus. Chromosome. Cytoplasm. Secreted.

Protein Families

HMGB family

Tissue Specificity

Expressed in gastric and intestinal tissues (at protein level).

Database Links

HGNC: 5000

OMIM: 163906

KEGG: hsa:3148

STRING: 9606.ENSP00000296503

UniGene: Hs.434953

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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