HNRNPM Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA901541A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: SW620 whole cell lysate(30µg), Hela whole cell lysate(30µg), HEK293T whole cell lysate(30µg), Jurkat whole cell lysate(30µg), NTERA-2 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: hnRNP M antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 78 kDa Observed band size: 78 kDa Exposure time：1min IHC image of CSB-RA901541A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA901541A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-RA901541A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA901541A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P52272

Target Names

HNRNPM

Alternative Names

Heterogeneous nuclear ribonucleoprotein M (hnRNP M), HNRNPM, HNRPM NAGR1

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human HNRNPM protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14D3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Heterogeneous nuclear ribonucleoprotein M (hnRNP M) plays a central role in pre-mRNA processing, alternative splicing regulation, and mRNA metabolism. As a member of the hnRNP family, this RNA-binding protein influences gene expression at the post-transcriptional level and has been implicated in cancer progression, making it a valuable target for researchers investigating splicing mechanisms, RNA biology, and oncogenic pathways.

This recombinant monoclonal antibody against HNRNPM offers the reproducibility and consistency that demanding experimental workflows require. Generated using recombinant technology with a defined sequence, clone 14D3 eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human HNRNPM, provides reliable detection across human and mouse samples.

Validation data demonstrates robust performance across multiple applications. Western blot analysis detects a clean band at the predicted 78 kDa molecular weight across diverse human cell lines including SW620, HeLa, HEK293T, Jurkat, and NTERA-2, as well as mouse NIH/3T3 cells, confirming cross-species reactivity and consistent target recognition. Immunohistochemistry staining has been validated in paraffin-embedded human glioma and liver tissues, supporting studies in both cancer and normal tissue contexts. Immunofluorescence analysis in HepG2 cells reveals the expected nuclear localization pattern characteristic of hnRNP family members, while flow cytometry validation in Jurkat cells demonstrates clear signal separation from isotype controls.

This versatility across Western blot, immunohistochemistry, immunofluorescence, and flow cytometry applications makes this antibody well-suited for researchers studying RNA processing mechanisms, splicing regulation in disease models, or hnRNP M's emerging roles in tumor biology.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.

Gene References into Functions

Overexpression of hnRNPM promotes breast cancer aggressiveness by regulating the level of CD44s and indicates a poor prognosis. PMID: 28393427
Data suggest that both heterogeneous nuclear ribonucleoprotein type M (HNRPM) and solute carrier 1A5 (SLC1A5) have role in the pathogenesis of ovarian cancer. PMID: 28609484
p54nrb and hnRNPM knockdown silences the FGF1 promoter-dependent accumulation of mRNA during myoblast differentiation. PMID: 26332123
Results suggest that glyceraldehyde-modified hnRNPM alters gene expression in nonalcoholic fatty liver disease. PMID: 25684943
The authors propose that cleavage of and subverting the function of hnRNPM by 3C protease is a general strategy utilized by picornaviruses to facilitate viral infection. PMID: 25926642
our data suggest that TAF15 and TLS/FUS operate within similar but not identical hnRNP M-TET protein complexes to influence the transcriptional or post-transcriptional output of a particular cell type. PMID: 24474660
findings demonstrate a novel molecular mechanism through which tumor metastasis is endowed by the hnRNPM-mediated splicing program. PMID: 24840202
Our results provide a clue that hnRNP M is a potential therapeutic target for Spinal muscular atrophy PMID: 24533984
The present data demonstrate that the upregulation of HnRNP M is involved in human colorectal epithelial carcinogenesis and may serve as a carcinoma biomarker for colorectal cancer. PMID: 24381081
Mechanistic control of carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) splice isoforms by the heterogeneous nuclear ribonuclear proteins hnRNP L, hnRNP A1, and hnRNP M. PMID: 21398516
A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. PMID: 20467437
PSF (polypyrimidine tract-binding protein-associated splicing factor) and p54(nrb), two highly related proteins involved in transcription and RNA processing, are identified as new binding partners of hnRNP M. PMID: 19874820
mitochondrial ribosomal protein S12 3'-UTR interacts specifically with TRAP1 (tumor necrosis factor receptor-associated protein1), hnRNPM4 (heterogeneous nuclear ribonucleoprotein M4), Hsp70 and Hsp60 (heat shock proteins 70 and 60), and alpha-tubulin PMID: 18790094

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Subcellular Location

Nucleus, nucleolus.

Database Links

HGNC: 5046

OMIM: 160994

KEGG: hsa:4670

STRING: 9606.ENSP00000325376

UniGene: Hs.465808

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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