Product Details

Uniprot No.

Target Names

HPGD

Alternative Names

15-hydroxyprostaglandin dehydrogenase [NAD(+)] (15-PGDH) (EC 1.1.1.141) (Prostaglandin dehydrogenase 1) (Short chain dehydrogenase/reductase family 36C member 1), HPGD, PGDH1 SDR36C1

Species Reactivity

Human

Immunogen

A synthesized peptide from human HPGD protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6H5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

HPGD, also known as 15-hydroxyprostaglandin dehydrogenase, serves as the primary enzyme responsible for the biological inactivation of prostaglandins. By catalyzing the NAD+-dependent oxidation of prostaglandins, HPGD plays a critical role in regulating inflammatory responses, tissue homeostasis, and has emerged as a significant tumor suppressor in multiple cancer types. Decreased HPGD expression has been associated with progression in colorectal, lung, and other carcinomas, making it an important target for researchers investigating prostaglandin signaling pathways and cancer biology.

This recombinant monoclonal antibody, generated from clone 6H5 in rabbit host, offers the reproducibility and consistency that demanding research applications require. Because the antibody sequence is defined and production occurs through recombinant expression, you can expect reliable performance across experiments and between lot numbers, eliminating the variability often encountered with traditional hybridoma-derived antibodies.

Validation studies demonstrate robust performance across multiple platforms. Western blot analysis of A549 whole cell lysate reveals a clean band at the predicted 29 kDa molecular weight, confirming specific detection of HPGD at dilutions between 1:500 and 1:2000. Flow cytometry applications have been validated using PC-3 cells, with clear separation between specific staining and isotype control populations, supporting intracellular detection workflows at dilutions of 1:50 to 1:200. The antibody is also suitable for ELISA-based quantification studies.

Whether you are investigating prostaglandin metabolism in inflammatory disease models, exploring HPGD as a tumor suppressor, or characterizing expression patterns in human cell lines, this antibody provides a dependable tool for advancing your research into this metabolically significant enzyme.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Primary enzyme catalyzing the conversion of hydroxylated arachidonic acid species to their corresponding oxidized metabolites (Probable). Prostaglandin inactivation, catalyzes the first step in the catabolic pathway of the prostaglandins. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Converts 11(R)-HETE to 11-oxo-5,8,12,14-(Z,Z,E,Z)-eicosatetraenoic acid (ETE). Has hydroxylated docosahexaenoic acid metabolites as substrates. Converts resolvins E1, D1 and D2 to their oxo products which represents a mode of resolvins inactivation and stabilizes their anti-inflammatory actions.

Gene References into Functions

Higher enzyme levels of 15-PGDH exist in the lumen of intracranial aneurysms of men compared with women. This observation could explain why aspirin confers better protection against IA rupture in men than in women. PMID: 29042428
HPGD expression in colorectal cancer and its role in cetuximab resistance PMID: 28320945
Results found that low 15-PGDH expression was significantly associated with advanced tumors, presence of lymph node metastasis and invasion, and poor prognosis in pancreatic ductal adenocarcinoma patients. PMID: 29224225
The reduced 15-PGDH may result in PGE2 accumulation which sustains carcinogenesis and tumor progression. We further found that miR-21 exert its oncogenic role through PGE2/PI3K/Akt/Wnt/beta-catenin axis in gastric cell proliferation. In conclusion, our findings enlarged our knowledge in the roles of miR-21 in the progression of gastric cancer. PMID: 29101039
WNT5A signaling regulates 15-PGDH expression. PMID: 27522468
miR-21-HPGD regulatory module may play an important role as part of a feed-forward loop that regulates the PGE2 signaling. Such a feed-forward regulatory mechanism likely plays a critical role in OTSCC initiation and progression. PMID: 27561985
inhibitory effects of 17-AAG on PGE2 levels in HT-29 colorectal cancer cells were mediated through modulating COX-2 and 15-PGDH expression. PMID: 27075590
A common mutation and a novel mutation in HPGD gene were identified to be responsible for primary hypertrophic osteoarthropathy PMID: 26135126
Peroxisome proliferator-activated receptors (PPARs) play pivotal roles in maintenance of Chorionic NAD-dependent 15-hydroxy prostaglandin dehydrogenase (PGDH) expression in chorion during human pregnancy. PMID: 26093984
Neoadjuvant chemotherapy could increase 15-PGDH expression in advanced gastric cancer patients, and 15-PGDH may serve as a candidate prognostic biomarker of advanced GC response to therapy. PMID: 26261578
15-PGDH mRNA levels were significantly higher in aorta samples from patients undergoing abdominal aortic aneurysm repair than in those from healthy multiorgan donors. PMID: 26287481
a homozygous 2-bp deletion (c.310_311delCT or p.L104AfsX3) was identified two primary hypertrophic osteoarthropathy siblings PMID: 24533558
Omega-3 polyunsaturated fatty acids upregulate the expression of 15-PGDH by inhibiting miR-26a and miR-26b. PMID: 25691459
Multiple drug resistance-associated protein 4 (MRP4), prostaglandin transporter (PGT), and 15-hydroxyprostaglandin dehydrogenase (15-PGDH) as determinants of PGE2 levels in cancer. PMID: 25433169
Loss of PGDH expression is associated with esophageal squamous cell carcinoma and adenocarcinoma. PMID: 25735395
A single nucleotide polymorphism in the 3' untranslated region (UTR) of the 15-hydroxyprostaglandin dehydrogenase (HPGD) gene modifies mir-485-5p binding in breast cancer. PMID: 25003827
Case Reports: novel 2-bp homozygous deletion was found in exon 3 (c.310-311delCT) of HPGD gene in the patients with Primary hypertrophic osteoarthropathy. PMID: 24816859
Tissues from 15-PGDH knockout mice demonstrate similar increased regenerative capacity. PMID: 26068857
Hypertrophic osteoarthropathy in the Chinese family is caused by a homozygous mutation (c.310_311delCT) in the HPGD gene. PMID: 25863089
Data show that hydroxylated Omega-3 fatty acid species are conferred with cell signaling capabilities after oxidation by 15-Hydroxyprostaglandin dehydrogenase (15PGDH). PMID: 25586183
these findings suggest that 15 d-PGJ2 induces the expression of 15-PGDH through ROS-mediated activation of ERK1/2 and subsequently Elk-1 in the MDA-MB-231 cells PMID: 25773924
Cholangiocarcinogenesis and tumor progression are regulated by a novel interplay between COX-2/PGE2 and miR-21 signaling, which converges at 15-PGDH. PMID: 24699315
Genetic variability in key genes in prostaglandin E2 pathway (COX-2, HPGD, ABCC4 and SLCO2A1) and their involvement in colorectal cancer development. PMID: 24694755
15-PGDH expression level in normal colon mucosa may serve as a biomarker that may predict stronger benefit from aspirin chemoprevention. PMID: 24760190
15-PGDH/15-KETE stimulates the cell cycle progression and proliferation of pulmonary arterial smooth muscle cells involving ERK1/2-mediated PAR-2 expression, and contributes to hypoxia-induced pulmonary vascular remodeling. PMID: 24657469
15-PGDH is downregulated in human hepatoma cells with a high COX-2 expression, in chemical and genetic murine models of hepatocellular carcinoma (HCC) and in human HCC biopsies. PMID: 23954207
The 15-PGDH gene is a MiTF-CX target gene in cervical stromal cells and is down-regulated by PGE2 through EP2 receptors. PMID: 24471568
The T allele showed increased cancer risk and decreased 15-PGDH expression. PMID: 23717544
Decreased PGDH expression is associated with increased GR and PRA, although decreased PRB, in chorion during labor. PMID: 23506845
Colonic 15-PGDH levels are highly reproducible within individuals and stable along the length of the colon PMID: 23625286
H. pylori appeared to promote gastric carcinogenesis by suppressing 15-PGDH. PMID: 23430757
beta-catenin has a novel role in promoting colorectal tumorigenesis through very early 15-PGDH suppression leading to increased PGE(2) levels, possibly even before COX-2 upregulation. PMID: 22082586
protein tyrosine nitration of 15-hydroxy prostaglandin dehydrogenase in the human mast cells PMID: 22197745
Data suggest that reduction of 15-PGDH is associated with carcinogenesis and development of gastric carcinoma. PMID: 22416177
Our results indicate that HPGD is highly expressed in metastatic and aggressive breast cancer and promotes epithelial-mesenchymal transition and migration in breast cancer cells. PMID: 22072156
clinical and biochemical data of three unrelated primary hypertrophic osteoarthropathy families with HPGD mutations; evidence that c.175_176del is a recurrent mutation rather than an ancient founder allele PMID: 21426412
Report homozygous mutations in the 15-hydroxyprostaglandin dehydrogenase gene in patients with primary hypertrophic osteoarthropathy. PMID: 19306095
Results demonstrate that 15-PGDH acts as a tumor suppressor in gastric cancer and provide further validation for 15-PGDH as a potential therapeutic target for gastric cancer. PMID: 20699658
Demonstrate both downregulation and a tumor suppressor activity of 15-PGDH in gastric cancer. PMID: 21469975
The PLA2G7, HPGD, EPHX2, and CYP4F8 genes are highly expressed in prostate cancer. PMID: 21281786
The c.175_176delCT frameshift mutation appears to be recurrent and to be the commonest HPGD mutation in Caucasian families with primary hypertrophic osteoarthropathy. PMID: 20299379
Data show that the Vmax for the oxidation of PGE2 was 28.1 micromol/(min.mg), and the catalytic constant, kcat, was about 14 per second; the catalytic efficiency, kcat/Km, was 2.5.106. PMID: 21072165
our data do not support the previously reported associations of HPGD tagSNPs and risk of colorectal cancer. PMID: 21047993
Reduced expression of 15-PGDH contributes to the elevated levels of PGs found in the skin following UVR exposure. PMID: 20643784
The study shows that cPLA2 and mPGES-1, in addition to COX-2, are constitutively overexpressed, and that 15-PGDH might be attenuated in colorectal cancer. Furthermore, cPLA2 and 15-PGDH as well as COX-2 could have an important role in tumor progression. PMID: 20635443
analysis of expression of serum vitamin D receptor, cyclooxygenase-2, and 15-hydroxyprostaglandin dehydrogenase in benign and malignant ovarian tissue and 25-hydroxycholecalciferol and prostaglandin E2 in ovarian cancer patients PMID: 20304053
These results suggest that enhanced PGE2 production proceeds through the expressions of COX-2 and microsomal PGES-1 and down-regulation of PGDH by SNAI2 in pancreatic tumors. PMID: 19820419
Loss of PGDH expression contributes to a more malignant bladder cancer phenotype and may be necessary for bladder cancer development and/or progression. PMID: 20093479
Genetic variants in HPGD encoding 15-PGDH, appear to modulate colorectal risk. The present study appears to be the first to evaluate possible associations between genetic heterogeneity in HPGD and CRC risk. PMID: 20042636
reduction of 15-PGDH is an independent predictor of poor survival associated with enhancement of cell proliferation in gastric adenocarcinoma. PMID: 19917058

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Involvement in disease

Hypertrophic osteoarthropathy, primary, autosomal recessive, 1 (PHOAR1); Cranioosteoarthropathy (COA); Isolated congenital nail clubbing (ICNC)

Subcellular Location

Cytoplasm.

Protein Families

Short-chain dehydrogenases/reductases (SDR) family

Tissue Specificity

Detected in colon epithelium (at protein level).

Database Links

HGNC: 5154

OMIM: 119900

KEGG: hsa:3248

STRING: 9606.ENSP00000296522

UniGene: Hs.596913

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Code	CSB-RA914804A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: A549 whole cell lysate(30µg) All lanes: HPGD antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time：1min Overlay Peak curve showing PC-3 cells stained with CSB-RA914804A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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HPGD Recombinant Monoclonal Antibody

Product Details

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Target Background