Product Details

Uniprot No.

Target Names

HSD3B1

Alternative Names

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I) (3-beta-HSD I) (Trophoblast antigen FDO161G) [Includes: 3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC 1.1.1.145) (3-beta-hydroxy-5-ene steroid dehydrogenase) (Progesterone reductase), Steroid Delta-isomerase (EC 5.3.3.1) (Delta-5-3-ketosteroid isomerase)], HSD3B1, 3BH HSDB3A

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human HSD3B1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10G2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Through the utilization of in vitro expression systems, the HSD3B1 recombinant monoclonal antibody is synthesized by cloning DNA sequences of HSD3B1 antibodies sourced from immunoreactive rabbits. The immunogen employed in this process is a synthesized peptide derived from the human HSD3B1 protein. Subsequently, the genes encoding the HSD3B1 antibodies are inserted into plasmid vectors, and these recombinant plasmid vectors are then transfected into host cells to enable antibody expression. The HSD3B1 recombinant monoclonal antibody undergoes affinity-chromatography purification and is rigorously tested for functionality in ELISA, IHC, IF, and FC applications, displaying reactivity with the human HSD3B1 protein during these assessments.

HSD3B1 is a key enzyme in steroidogenesis, catalyzing the conversion of pregnenolone to progesterone. Its activity is essential for the production of various steroid hormones, including sex steroids, glucocorticoids, and mineralocorticoids, which have critical roles in reproductive function, stress response, and various physiological processes in both males and females.

Target Background

Function

A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity.

Gene References into Functions

High HSD3B1 Expression is Associated with Cancer Progression in Breast Cancer. PMID: 28744792
Fungicide ziram may disrupt the placental steroid production. In the present study, we investigated the effects of ziram on steroid formation in human placental cell line JEG-3 cells and on HSD3B1 and aromatase in the human placenta. Ziram did not inhibit progesterone production in JEG-3 cells and HSD3B1 activity at 100muM... ziram is a potent inhibitor of human aromatase. PMID: 28951168
This is the first study to indicate that 3beta-HSD type 1 is a potent prognostic factor in hormone-dependent breast cancer. PMID: 27139182
HSD3B1 is involved in aldosterone production, despite its lower levels of expression compared with HSD3B2, and also possibly associated with KCNJ5 mutation in aldosterone-producing adenoma. PMID: 25458695
Expression of the genes HSD3B1, HSD17B3, and SRD5A2 was significantly increased in BPH tissues compared to normal adjacent prostate tissues. PMID: 24810473
prostate cancer patients who harbored the heterozygous variant HSD3B1 (1245C) are more likely to develop to castration-resistant prostate cancer but do not have shorter time to biochemical recurrence, shorter survival time or higher mortality risk PMID: 25731771
No evidence to support that HSD3B1 is involved in aldosterone synthesis in the human adrenal cortex or that genetic variation in HSD3B1 affects blood pressure or hypertension. PMID: 24951726
Results indicate that orexin-A significantly enhanced the expression of 3beta-hydroxysteroid dehydrogenase (3beta-HSD) and the production of cortisol, and increased the phosphorylation of AKT protein in the NCI-H295R adrenocortical cells. PMID: 25319929
The data of this study supports a strongly positive association between mitochondrial complex I gene variations and MS pathogenesis in a Filipino population. PMID: 25172194
The AAT haplotype of the HSD3B1 gene was significantly associated with increased risks of acne vulgaris in Han Chinese from the Southwest China. PMID: 24157973
HSD3B1 T-->C Leu338, HTR2A T102C, GNAS T393C, and RGS2 G638A polymorphisms were not associated with hypertension risk. PMID: 23859711
Risk-conferring genetic variations in the HSD3beta gene influenced susceptibility of primary aldosteronism. Concomitant presence of rs6203 CC and rs12410453 GA genotypes synergistically increased aldosterone-to-renin ratio PMID: 24006038
Study shows that castration-resistant prostate cancer sometimes expresses a gain-of-stability mutation that leads to a gain-of-function in 3betaHSD1, which catalyzes the initial rate-limiting step in conversion of the adrenal-derived steroid dehydroepiandrosterone to dihydrotestosterone. PMID: 23993097
elevated in placental tissue of women with polycystic ovarian syndrome PMID: 23122578
HSD3B1 is a highly specific trophoblast-associated marker that can be used in the distinction of trophoblastic tumorlike lesions and tumors from nontrophoblastic lesions and tumors. PMID: 23318910
The aim of this haplotype-based case-control study was to estimate whether polymorphisms of the maternal estrogen synthesis genes (CYP19A1, HSD3B1 and HSD3B2) are associated with preeclampsia and gestational hypertension PMID: 22638611
Carriers of HSD3B1 GCC haplotype had lower peak early (Ea; P = 0.004) and higher peak late (Aa; P = 0.066) diastolic mitral annular velocities and therefore a lower Ea/Aa ratio (P = 0.041) as compared with noncarriers PMID: 22673022
The HSD3B1 T/C polymorphism cannot be used as genetic marker for the risk for recurrent spontaneous abortions in our Caucasian population. PMID: 21631238
There is expression of 3beta-Hsd1 in XX gonads during gonad differentiation period. PMID: 21932034
rs6203 and rs1047303 in the HSD3B1 gene are useful genetic markers for essential hypertension, while polymorphisms of HSD3B1 are associated with the BP and aldosterone level. PMID: 20660004
The study identifies an amino acid in the steroid binding domain of human 3-beta-HSD I that may be exploited to produce new inhibitors that are much more highly specific for 3-beta-HSD I in breast tumors compared to adrenal 3-beta-HSD II. PMID: 20420909
We investigated associations between single nucleotide polymorphisms in genes HSD3B1, SRD5A1/2, and AKR1C2 and prostate cancer risk PMID: 20056642
Data indicate that enzymes CYP17A1 and HSD3B1 showed low expression, while AKR1C3 and SRD5A1 were abundantly expressed. PMID: 20086173
nonstop mutation in the stop codon and missense mutation in type II 3beta-hydroxysteroid dehydrogenase gene causing 3beta-HSD deficiency congenital adrenal hyperplasia. PMID: 12050213
Polymorphism in exon 4 of the human gene and blood presssure PMID: 12054649
Structure/function relationships responsible for the kinetic differences between isoforms PMID: 12205101
HSD3B1 was identified using a trifunctional phenyl sulfonate probe. PMID: 12438565
Substantially higher affinity of 3beta-HSD1 activity for substrate and inhibitor steroids relative to the 3beta-HSD2 enzyme. PMID: 12530651
In outer region of adrenal cortex immunoreactivity was observed for 3beta-hydroxysteroid dehydrogenase. PMID: 12530676
Structure/function relationships responsible for coenzyme specificity and the isomerase activity of this multienzyme complex. PMID: 12832414
Descending HSD3B phenotype in hyperandrogenic females is associated with a variant of insulin-resistant polycystic ovary syndrome. PMID: 14764797
Neither of the HSD3B1 or PTP1B variants were associated with hypertension PMID: 15097232
results designate YY1 as the factor responsible for the intron 1-mediated boost of the HSD3B2 gene basal promoter activity PMID: 15291746
further characterizes structure/function relationships of human 3beta-HSD and bring us closer to the goal of selectively inhibiting the type 1 enzyme in placenta to control the timing of labor or in hormone-sensitive breast tumors to slow their growth PMID: 15291757
identification of structural reasons for the substantially higher affinities of 3beta-HSD1 for substrates, coenzymes, and inhibitors PMID: 15797861
The Rossmann-fold domain of 3beta-HSD1 contains two Cys residues, Cys72 and Cys111, which are capable of forming an intrasubunit disulfide bond based on their proximity in our structural model. PMID: 17624763
Variant in the HSD3B1 gene is associated with increased mammographic density PMID: 17627014
There is a possible role in human disease of common genetic variation in HSD3B1 and HSD3B2. PMID: 17689071
results suggest that the HSD3B1 N367T and UGT2B17 null polymorphisms may modify the risk of prostate cancer, particularly among men with a family history of the disease PMID: 17826523
HSD3B1 is highly specific and sensitive compared with other trophoblastic markers dor differential diagnosis of trophoblastic tumors and tumorlike lesions.. PMID: 18223326
Structure/function of the inhibition of HSD3B1 by trilostane is reported. PMID: 18524572
The activity levels of 17beta-hydroxysteroid dehydrogenase (17beta-HSD), 3beta-hydroxysteroid dehydrogenase (3beta-HSD), 3alpha-hydroxysteroid dehydrogenase (3alpha-HSD/3-KSR) and estrone sulfatase in ovarian epithelial carcinomas, were assayed. PMID: 18723074
Anti-inflammatory effects of IL-1alpha and IL-4 on 3beta-HSD2 mRNA involve a p38 MAPK signalling pathway, whereas pro-inflammatory response of IL-1alpha to 3beta-HSD1 mRNA involves a NF-kappaB inflammatory pathway. PMID: 18778748
The high affinity, competitive inhibition of 3beta-HSD1 by trilostane and epostane may be related to the presence of Arg195 in 3beta-HSD1 vs. Pro195 in 3beta-HSD2. PMID: 18955108
The genetic polymorphisms of the HSD3B1,genes were found to be significantly different (p<0.05) between the uremic and non-uremic diabetes group PMID: 19148546
Among patients with essential hypertension, cholesterol side-chain cleavage & MDR1 loci are related to circulating endogenous ouabain & DBP. In contrast, variants in HSD3B1 are related with SBP probably via aldosterone PMID: 19197249
3beta-HSD protein was immunodetectable in primary ascites of women who were diagnosed with epithelial ovarian cancer but mRNA transcripts of both 3beta-hydroxysteroid dehydrogenase type 1 and type 2 were diminished relative to normal cells. PMID: 19414525
compared (+)- and (-)-gossypols in the inhibition of 3beta-HSD and 17beta-HSD3 in human and rat testes PMID: 19429456

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Subcellular Location

Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.

Protein Families

3-beta-HSD family

Tissue Specificity

Placenta and skin. Predominantly expressed in mammary gland tissue.

Database Links

HGNC: 5217

OMIM: 109715

KEGG: hsa:3283

STRING: 9606.ENSP00000358421

UniGene: Hs.364941

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Code	CSB-RA071064A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA071064A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.50% DAB. IHC image of CSB-RA071064A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.50% DAB. Immunofluorescence staining of Hela with CSB-RA071064A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 514-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing 293 cells stained with CSB-RA071064A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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HSD3B1 Recombinant Monoclonal Antibody

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