HSP90AA1/HSP90AB1 Antibody

Datasheet
Code CSB-RA010802A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue
    All lanes: Hsp90 alpha + beta antibody at 1.25μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 90 KDa
    Observed band size: 90 KDa

  • Immunoprecipitating Hsp90 alpha + beta in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-RA010802A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA010802A0HU (3μg) + Hela whole cell lysate (500μg)
    Lane 3: Hela whole cell lysate (20μg)

  • Overlay histogram showing Jurkat cells stained with CSB-RA010802A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

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Product Details

Uniprot No. P07900P08238
Target Names HSP90AA1/HSP90AB1
Species Reactivity Human, Rat
Immunogen A synthesized peptide derived from human HSP90AA1/HSP90AB1
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, FC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IP 1:200-1:1000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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