HSP90AA1/HSP90AB1 Recombinant Monoclonal Antibody

Code CSB-RA010802A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue
    All lanes: Hsp90 alpha + beta antibody at 1.25μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 90 KDa
    Observed band size: 90 KDa
  • Immunoprecipitating Hsp90 alpha + beta in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-RA010802A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA010802A0HU (3μg) + Hela whole cell lysate (500μg)
    Lane 3: Hela whole cell lysate (20μg)
  • Overlay histogram showing Jurkat cells stained with CSB-RA010802A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Target Names
HSP90AA1/HSP90AB1
Species Reactivity
Human, Rat
Immunogen
A synthesized peptide derived from human HSP90AA1/HSP90AB1
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
10D6
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, FC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IP 1:200-1:1000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The HSP90AA1/HSP90AB1 recombinant monoclonal antibody is a highly specific antibody designed to target both HSP90AA1 and HSP90AB1 proteins. This antibody is engineered using advanced biotechnological techniques, including genetic engineering and antibody engineering. The production process involves cloning specific DNA sequences encoding the variable regions of the HSP90AA1/HSP90AB1 antibody heavy and light chains into a plasmid vector, followed by transfection into a host cell for expression. The resulting HSP90AA1/HSP90AB1 recombinant monoclonal antibody is purified through affinity chromatography from the cell culture supernatant. Extensive validation has been performed to demonstrate its efficacy in detecting both human and rat HSP90AA1 and HSP90AB1 proteins in four applications including ELISA, WB, FC, and IP.

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