IGKC Recombinant Monoclonal Antibody

The immunoglobulin kappa constant region (IGKC) represents a fundamental component of antibody structure, serving as the constant domain of kappa light chains that pair with heavy chains to form functional immunoglobulins. As kappa light chains constitute approximately 60% of human antibody light chains, IGKC serves as a valuable marker for studying B cell biology, plasma cell populations, and immunoglobulin-producing malignancies. Its consistent expression across diverse antibody-secreting cells makes it particularly useful for identifying and characterizing immune cell infiltrates in tissue samples.

This recombinant monoclonal antibody, clone 7E6, offers the reproducibility advantages that come with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that each lot delivers identical binding characteristics, eliminating the variability that can compromise longitudinal studies or multi-site research collaborations. For investigators building datasets over extended timeframes, this consistency translates directly into more reliable, comparable results.

Validation studies demonstrate robust performance in immunohistochemistry applications using formalin-fixed, paraffin-embedded human tissues. Testing in human tonsil tissue confirms reliable detection in lymphoid tissue rich in antibody-producing cells, while validation in human breast cancer tissue illustrates the antibody's utility for characterizing tumor-infiltrating immune populations. Both applications employed citrate buffer antigen retrieval at pH 6.0 with overnight primary antibody incubation, with recommended working dilutions ranging from 1:50 to 1:200 allowing optimization flexibility across different tissue types and detection systems.

This antibody supports immunology research programs investigating B cell differentiation, plasma cell disorders, and the tumor microenvironment, providing a dependable tool for researchers examining humoral immune responses and immunoglobulin expression patterns in health and disease.