KHSRP Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA130686A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: THP-1 whole cell lysate(30µg), K562 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), A431 whole cell lysate(30µg), HeLa whole cell lysate(30µg), HepG2 whole cell lysate(30µg), Jurkat whole cell lysate(30µg) All lanes: KHSRP antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 73 kDa Observed band size: 62 kDa Exposure time：1min IHC image of CSB-RA130686A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA130686A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A431 cell with CSB-RA130686A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA130686A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q92945

Target Names

KHSRP

Alternative Names

Far upstream element-binding protein 2 (FUSE-binding protein 2) (KH type-splicing regulatory protein) (KSRP) (p75), KHSRP, FUBP2

Species Reactivity

Human

Immunogen

A synthesized peptide from human KHSRP protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12H11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

KHSRP, also known as KH-type splicing regulatory protein or Far upstream element-binding protein 2, plays a critical role in post-transcriptional gene regulation. This multifunctional RNA-binding protein participates in mRNA decay, microRNA biogenesis, and alternative splicing, making it a key player in cellular processes ranging from immune responses to cancer progression. Researchers investigating RNA metabolism, inflammatory signaling, or tumor biology will find KHSRP an essential target for understanding these interconnected pathways.

This recombinant monoclonal antibody, clone 12H11, offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit host and monoclonal nature ensure high specificity for human KHSRP, providing confidence in your results.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis successfully detects KHSRP across a diverse panel of human cell lines including THP-1, K562, U-251MG, A431, HeLa, HepG2, and Jurkat cells, with the observed band at 62 kDa running slightly below the predicted 73 kDa molecular weight—a difference likely attributable to alternative splicing or protein processing. Immunohistochemistry staining has been validated in human breast cancer and kidney tissue sections, while immunofluorescence confirms nuclear localization in A431 cells. Flow cytometry analysis in Jurkat cells demonstrates clear positive signal separation from isotype controls.

Whether you are exploring KHSRP's role in mRNA stability, investigating its function in cancer biology, or studying immune cell regulation, this antibody provides a reliable foundation for your research across multiple experimental approaches.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Binds to the dendritic targeting element and may play a role in mRNA trafficking. Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

Gene References into Functions

KSRP, miR-129, and RUNX1 participate in a regulatory axis to control the outcome of myeloid differentiation. PMID: 29127290
The authors find that KHSRP associates with the regulatory domain of RIG-I to maintain the receptor in an inactive state and attenuate its sensing of viral RNA (vRNA). PMID: 28248290
we discovered a novel mechanism underlying SUMOylation of KHSRP regulating the production of some special miRNAs. PMID: 29020972
KSRP decreased EGR3 mRNA stability in an ARE-independent manner. PMID: 28847731
TGF-beta-induced KHSRP silencing is central in a pathway leading to gene-expression. PMID: 27396342
mechanistic evidence that KSRP promotes the down-regulation of Spry4 by a previously unidentified mechanism, i.e. post-transcriptional mRNA regulation. PMID: 28275056
The interaction between the KLHL12 and the C-terminal domain of KHSRP contributed to KHSRP ubiquitination, leading to downregulation of enterovirus internal ribosome entry sites-mediated translation in infected cells. PMID: 27899653
The miRNA biogenesis factors, DDX17 and KHSRP, regulate the protein level of Ago2 in human cells. PMID: 27478153
Results show that KHSRP protein is highly expressed in osteosarcoma cells and provide evidence for its important roles in regulatory controls of osteosarcoma pathogenesis. PMID: 27573585
High FBP2 expression is associated with doxorubicin resistance in breast cancer . PMID: 26810065
Further analyses indicated that KHSRP was involved in miR-26a maturation and inhibited the expression of PTEN in small cell lung cancer cells PMID: 27644194
The expression of KSRP in liver epithelial cells controlled by mir-27b. PMID: 26631623
Khsrp as a highly regulated RBP in response to genotoxic stress. PMID: 25993413
The ability of KSRP to integrate different levels of gene expression is required for proper immune response. [Review] PMID: 24845017
subcellular localization of KSRP is regulated by competing interactions with DDX1 or 14-3-3 PMID: 24023901
KSRP induces enhanced processing of pri- and pre-miR-155 in cystic fibrosis lung epithelial cells. PMID: 23524258
Enterovirus 71 infection cleaved FBP2, which altered its function when its carboxyl terminus was cleaved. PMID: 23345520
Binding of the human KSRP protein to let-7 miRNA precursors positively regulates their processing to mature let-7, thereby contributing to control of cell proliferation, apoptosis and differentiation PMID: 23142982
The role of KSRP in mRNA decay and microRNA precursor maturation. PMID: 21935887
RACK1 binds to KH-type splicing regulatory protein (KSRP), a member of the Dicer complex, and is required for the recruitment of mature miRNAs to the RNA-induced silencing complex (RISC). PMID: 21935400
KHSRP was identified as being involved in the regulation of rpL3 gene expression via alternative pre-mRNA splicing. PMID: 21705779
KSRP-dependent silencing was reversed by IL-1, a strong inducer of IL-6 mRNA and protein expression. PMID: 21795706
KSRP binds to the terminal loop sequence of a subset of miRNA precursors promoting their maturation (REVIEW) PMID: 21627028
In this review we will discuss in detail KSRP ability to i) promote decay of labile mRNAs interacting with some components of the mRNA decay machinery and ii) favor the maturation of a select group of microRNA precursors[review] PMID: 21196264
hnRNP A1 and KSRP have antagonistic roles in the post-transcriptional regulation of let-7a expression. PMID: 20639884
Dishevelled-KSRP complex operates in Wnt regulation of beta-catenin, functioning post-transcriptionally upon CTNNB1 mRNA stability. PMID: 20332102
KSRP do indeed interact with the c-src transcript in vivo, and that these associations change with the differentiated state of the cell. PMID: 14657238
KSRP, a KH domain-containing ARE-BP, is an essential factor for ARE-directed mRNA decay. Some KH motifs of KSRP mediate RNA binding, mRNA decay, and interactions with the exosome. PMID: 15175153
IL-8 RNA from IL-1beta-stimulated cytoplasmic extract revealed a 20-fold greater association of transcript with the stabilizing factor HuR vs. the destabilizing factor KSRP in breast cancer cells PMID: 15514971
KHSRP, tethered to mRNAs, elicits mRNA decay. PMID: 16648466
These results indicate that KSRP functions as a limiting factor in inflammatory gene expression. PMID: 17908789
These data indicate a novel role for KSRP in parathyroid hormone gene expression. PMID: 18583400
The over-expression of a novel protein family, far upstream binding proteins (FUBPs) was identified in both stages of hepatocellular carcinoma and confirmed by western blots. PMID: 19003864
KSRP serves as a component of both Drosha and Dicer complexes and regulates the biogenesis of a subset of miRNAs PMID: 19458619
The coordinated activation of FBP-1 and FBP-2 represents a novel and frequent pro-tumorigenic mechanism promoting proliferation (tumor growth) and motility (dissemination) of human liver cancer cells. PMID: 19585652
Knock-down of either exosome components or KSRP by siRNAs prevents PMR1-mediated cleavage of PTH mRNA. PMID: 19775426

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

KHSRP family

Tissue Specificity

Detected in neural and non-neural cell lines.

Database Links

HGNC: 6316

OMIM: 603445

KEGG: hsa:8570

STRING: 9606.ENSP00000381216

UniGene: Hs.727344

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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