Code | CSB-RA956956A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
After immunizing animals with a synthesized peptide derived from human KIF11, B cells were isolated and fused with myeloma cells to generate hybridomas for KIF11 antibody production. The KIF11 antibody gene was then sequenced and cloned into a vector. The KIF11 monoclonal antibody gene-containing vector was transfected into cells for cultivation, and the resulting KIF11 recombinant monoclonal antibody was isolated and purified using affinity chromatography from the cell culture supernatant. The purified antibody has been specifically tested and shown to react with only human KIF11 samples in both ELISA and WB applications.
The KIF11 protein, also known as Eg5, is a motor protein involved in the organization of the mitotic spindle and cell division. Specifically, KIF11 plays a critical role in the formation and function of the spindle apparatus during mitosis. It is responsible for the separation of duplicated chromosomes, by moving the microtubules apart, allowing for proper distribution of genetic material to daughter cells during cell division. KIF11 is also involved in the maintenance of the spindle checkpoint, which ensures that each daughter cell receives an accurate and complete set of chromosomes. It is also implicated in other cellular processes such as axon formation and maintenance, intracellular transport, and neuronal development.
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