KIF11 Recombinant Monoclonal Antibody

Code CSB-RA956956A0HU
Size US$210
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  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, U-251 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysat
    All lanes: Eg5 Antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 120 kDa
    Observed band size: 130 kDa
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Product Details

Uniprot No.
Target Names
KIF11
Alternative Names
Kinesin-like protein KIF11 (Kinesin-like protein 1) (Kinesin-like spindle protein HKSP) (Kinesin-related motor protein Eg5) (Thyroid receptor-interacting protein 5) (TR-interacting protein 5) (TRIP-5), KIF11, EG5 KNSL1 TRIP5
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human Eg5
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
1G11
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

After immunizing animals with a synthesized peptide derived from human KIF11, B cells were isolated and fused with myeloma cells to generate hybridomas for KIF11 antibody production. The KIF11 antibody gene was then sequenced and cloned into a vector. The KIF11 monoclonal antibody gene-containing vector was transfected into cells for cultivation, and the resulting KIF11 recombinant monoclonal antibody was isolated and purified using affinity chromatography from the cell culture supernatant. The purified antibody has been specifically tested and shown to react with only human KIF11 samples in both ELISA and WB applications.

The KIF11 protein, also known as Eg5, is a motor protein involved in the organization of the mitotic spindle and cell division. Specifically, KIF11 plays a critical role in the formation and function of the spindle apparatus during mitosis. It is responsible for the separation of duplicated chromosomes, by moving the microtubules apart, allowing for proper distribution of genetic material to daughter cells during cell division. KIF11 is also involved in the maintenance of the spindle checkpoint, which ensures that each daughter cell receives an accurate and complete set of chromosomes. It is also implicated in other cellular processes such as axon formation and maintenance, intracellular transport, and neuronal development.

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Target Background

Function
Motor protein required for establishing a bipolar spindle during mitosis. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.
Gene References into Functions
  1. PP2A controls mitotic exit through EG5 dephosphorylation. PMID: 28487562
  2. These data demonstrate the functional interplay between PTEN and EG5 in controlling mitotic spindle structure and chromosome behaviour during mitosis. PMID: 27492783
  3. the RNF20/40 complex, a major ubiquitin ligase catalysing histone H2B monoubiquitination, interacts with the motor protein Eg5 during mitosis and participates in spindle assembly. PMID: 27557628
  4. Stability of protein-ligand complexes were analyzed using molecular dynamic simulations. Our studies suggest that pyridazine analogs have good MDCK, permeability properties and high binding affinity to the human Eg5. PMID: 29041840
  5. These results illustrate how a posttranslational modification of a kinesin can be used to fine tune motor behavior to meet specific physiological needs. PMID: 29432173
  6. Immunohistochemical analysis demonstrated that KIF11 was expressed in 64 of 99 (64.6%) oral cancer tissues but not in healthy oral epithelia. Strong KIF11 expression was significantly associated with poor prognosis among oral cancer patients (P=0.034), and multivariate analysis confirmed its independent prognostic value. PMID: 29115586
  7. Results identified 7 novel KIF11 mutations during the screening of 142 patients with familial exudative vitreoretinopathy (FEVR), and further confirmed that KIF11 is causative of FEVR in an autosomal dominant manner. PMID: 27212378
  8. results demonstrate that Eg5 is phosphorylated in cultured cells by Src family kinases (SFKs) at three sites in the motor head: Y125, Y211, and Y231. Mutation of these sites diminishes motor activity in vitro, and replacement of endogenous Eg5 with phosphomimetic Y211 in LLC-Pk1 cells results in monopolar spindles, consistent with loss of Eg5 activity. PMID: 28646493
  9. Results show that KIF11 mRNA expression was upregulated in prostate cancer tissues and associated with aggressive characteristics. PMID: 27842896
  10. Eg5 is overexpressed in the livers of the hepatocellular carcinoma patients and is predictive of poor prognosis. PMID: 28684886
  11. The data suggested that Eg5 may be identified as a novel prognostic biomarker and targeting Eg5 seems to be a novel strategy for LSCC treatment. PMID: 27020495
  12. Progressive retinal degeneration in KIF11-related retinopathy indicates a role for KIF11 not only in ocular development but also in maintaining retinal morphology and function. The remarkable variability of the ocular phenotype suggests four different types of retinopathy which may overlap. PMID: 28785766
  13. Among the detected mutations, LRP5 accounted for the largest proportion with a mean mutation rate of 16.1% (5/31, 16.1%), followed by NDP (3/31, 9.7%), FZD4 (2/31, 6.5%), TSPAN12 (1/31, 3.2%), and KIF11 (1/31, 3.2%). All the novel changes were predicted to be pathogenic by a series of bioinformatics analyses. PMID: 28494495
  14. Data show that the kinesin 5 (Eg5) binding time has less sensitivity on MCF7 as compared to bovine brain microtubules. PMID: 27590585
  15. mixing of Eg5 and MTs produced a range of spatiotemporal dynamics PMID: 27463139
  16. that Pten, through the Dlg1-binding ability of its PDZ-binding domain , accumulates phosphorylated Eg5 at duplicated centrosomes to establish symmetrical bipolar spindles that properly segregate chromosomes PMID: 27240320
  17. KIF11 is an important regulator of activity in oesophageal and colorectal tumor stem cells. PMID: 28011472
  18. Levels of phosphorylated Erk1/2 were lower in KIF11 siRNA-transfected cells than with negative-control siRNA-transfected cells. These results indicate that KIF11 is involved in intestinal mucin phenotype gastric cancer. PMID: 27459100
  19. Patients with KIF11 mutations show a specific ocular phenotype with variable expressivity and intrafamilial variability. Macular atrophy and dysfunction have not been consistently documented before. PMID: 25996076
  20. This stuidy demonstrated that overexpression of Eg5 associates with high-grade astrocytic neoplasm. PMID: 26456023
  21. Data show that kinesin spindle protein Eg5 inhibitor LY2523355 has broad target-mediated anticancer activity in vitro and in vivo. PMID: 26304237
  22. Data show there was no significant change in Eg5 protein inhibitor treatment. PMID: 26658059
  23. the levels and distribution of TPX2 are likely to be determinants of when and where kinesin-5 acts in neurons. PMID: 26257190
  24. Targeting KIF11 also hit the other arm of the "go or grow" cell fate decision by reducing glioma cell invasion. PMID: 26355032
  25. Identification of mutations in 8.3% patients suggests KIF11 mutations as a common cause of familial exudative vitreoretinopathy (FEVR). PMID: 26472404
  26. This report, therefore, further expands the clinical and molecular spectrum of KIF11-associated microcephaly. PMID: 25115524
  27. Data show that kinesin spindle protein (KSP) inhibition sensitized chronic myeloid leukemia (CML) cells to imatinib-induced apoptosis. PMID: 25146433
  28. All inherited cases, and 50% of sporadic cases of MCLMR are due to germline KIF11 mutations. It is possible that mosaic KIF11 mutations cause the remainder of sporadic case PMID: 25934493
  29. Dimeric, but not monomeric, Eg5 was differentially inhibited by full-length and truncated TPX2, demonstrating that dimerization or residues in the neck region are important for the interaction of TPX2 with Eg5. PMID: 26018074
  30. This identified a heterozygous 2-base pair deletion (Thr65 del 2 base pair AT) in exon 2 that, to our knowledge, has not been previously reported. PMID: 25764055
  31. nuclear Eg5-expression may be a predictive biomarker for docetaxel response in metastatic castrate-resistant prostate cancer (PCa) patients and a prognostic biomarker for hormone-naive PCa patients. PMID: 25277178
  32. Study combines results from extensive molecular dynamics simulations with insights from bioinformatics analysis to reveal details about the internal dynamics of the kinesin-5 motor domain associated with allosteric regulation and inhibition PMID: 25418105
  33. Cryoelectron microscopy reconstructions indicate that allosteric inhibitors of human kinesin-5 bind to a motor conformation that occurs in the course of normal function. PMID: 23135273
  34. Eg5, Kif15, and dynein work together to build a bipolar spindle and reveal an important role for antagonistic motors in chromosome segregation. PMID: 25127142
  35. Cases of familial exudative vitreoretinopathy should be carefully inspected for the presence of microcephaly as a marker for KIF11-related disease to enhance the accuracy of the prognosis and genetic counseling. PMID: 25124931
  36. EG5 is essential for robust maintenance of spindle bipolarity in some human cell types. PMID: 24807901
  37. Microcephaly with or without chorioretinopathy, lymphoedema, or mental retardation (MCLMR): review of phenotype associated with KIF11 mutations. PMID: 24281367
  38. this study analyzes enastron and dimethylenastron interactions with ADP-bound Eg5 crystal structure, and find that dimethylenastron binds Eg5 motor domain with higher affinity. PMID: 24732354
  39. Identify Tat as a viral regulator of Eg5 and provide novel insights into the mechanisms of action of Tat in mediating the reduction of CD4-positive T-lymphocytes. PMID: 24488929
  40. alpha3. We propose that these features facilitate the production of sustained opposing force by Eg5, which underlies its role in supporting formation of a bipolar spindle in mitosis. PMID: 24145034
  41. The study presents ATP hydrolysis mechanisms in the kinesin-5 protein Eg5 by using combined quantum mechanics/molecular mechanics metadynamics simulations. PMID: 23751065
  42. Data show that five genes CKAP5, KPNB1, RAN, TPX2 and KIF11 were shown to be essential for tumor cell survival in both head and neck squamous cell carcinoma (HNSCC)and non-small cell lung cancer (NSCLC), but most particularly in HNSCC. PMID: 23444224
  43. Eg5 knockdown inhibits PAUF secretion in HeLa cells. PMID: 23857769
  44. Results indicate that ispinesib-induced structural changes in loop 5 (L5) from Eg5 lead to subsequent changes in the conformation of the switch II loop and helix and in the neck linker. PMID: 23658017
  45. In conclusion, this epistatic interaction showed a high degree of consistency when stratifying by sex, the epsilon4 allele of apolipoprotein E genotype, and geographic region. PMID: 23036584
  46. This finding suggested that Eg5 served as a prognostic factor in patients with renal cell carcinoma PMID: 23371254
  47. Premature separation of centrosomes decreases the requirement for the major mitotic kinesin Eg5 for spindle assembly. PMID: 23643362
  48. Eg5 directly interacts with NuMA in the vicinity of the spindle poles, although the interaction does not occur in interphase cells. PMID: 23368718
  49. Dynein most likely antagonizes Eg5 indirectly by exerting force at different spindle locations rather than through a simple push-pull mechanism at the spindle equator. PMID: 22832270
  50. The 2.6 A resolution structure has been determined for the ternary complex of Eg5 motor domain in complex with magnesium (Mg)2 + adenosine diphosphate (ADP) and ispinesib. PMID: 22993085

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Involvement in disease
Microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR)
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton, spindle pole.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, BimC subfamily
Database Links

HGNC: 6388

OMIM: 148760

KEGG: hsa:3832

STRING: 9606.ENSP00000260731

UniGene: Hs.8878

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